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TWiM regularly receives listener email with corrections, comments, suggestions for show topics, requests for clarification, and additional information. A selection of these is archived on this page.
I’m reeling from this episode!! The symbiosis, the web of life, the energies, chemicals, organisms of
evolutionary progression shaping our world naturally and by human intervention with unknown consequences, then you all tie it to the brain microbiome alterations affecting behavior! Yes!!!
A recent quote from a researcher says soon we’ll call our immune system the bacterial interaction system!
Yes! The microbes rule our world and us. God is bug. Oh the heresy!
This is what we see with inflammation therapy…bugs rule even if you can’t culture them with Koch’s postulates.
I have a Talmudic question:
If most alphaproteobacteria are phototrophic, and the brain doesn’t get sunshine, does vitamin D endocrine system dysregulation have an effect on their population in the brain?
Vitamin D hormone transcribes the AMPs to balance our bugs. https://chronicillnessrecovery.org/images/stories/How_Vitamin_D_Metabolism_Affects_Autoimmune_and_Inflammatory_Diseases2.pdf
Why we use olmesartan to correct vitamin D dysregulation.
Angiotensin and Systems Thinking: Wrapping Your Mind Around the Big Picture
[the following email was sent to TWiP but I think TWiM listeners will like it]
Vince and Dickson,
Here is a follow up on your biofuels question. To make fuels from crops succeed we need a biotech breakthrough that I really thought someone would have done already. Basically we need a bug that eats cellulose and converts it back to sugar so you can ferment it. Personally, I think there is a Nobel Prize in it for the group that creates an e. coli strain that converts cellulose to sugars. Once you have that, then you can feed the farm animals the corn and run your tractor on the corn stalks! Or you could use hay or grass clips or wood chips or waste paper, whatever is available cheaply. Until we get that bug, ethanol from corn will just be a niche technology.
People will keep pushing to use corn crops or other high sugar crops to make fuel, but the economics are not good and the lost opportunity costs are too high. Look how the modest current efforts in the USA have pushed up food prices and still required government subsidies to be competitive. No doubt there is a listener out there with lots of arguments for how great ethanol from corn is but I don't see anything like the margin needed to make it a viable market changing crude oil substitute.
I will show my age and tell you that as a senior chemical engineering design project in 1980 at Purdue, we looked at how to convert crop waste materials (like corn stalks) into fuel and it wasn't pretty. The only real way to break down the cellulose was to grind it up and treat it with hot fuming sulfuric acid in big reactors. Fuming sulfuric is 98% concentrated acid that is saturated with sulfur trioxide gas, brute force chemistry for sure! As I remember, you could get pretty good conversion of the cellulose, but the ugly part was separating the good stuff from all the waste acid and the non reactive lignin. Once you got all the acid out of the good stuff, then you still had to ferment the sugars. It is not surprising that you don't see anybody running this process to make fuel! We need a biotech solution to break down the cellulose without all the mess. I think folks were looking at the microbes in termites' stomachs as a place to start., but I have not heard of any progress on this in several years.
I will add that biofuels are not the only option for our fuel supply. For the past 100 years, we have had repeated dramatic reports that we are about to run out of oil and yet it never seems to happen! I remember a particularly detailed one in Scientific American about 10-15 years ago with beautiful graphs and everything. Each time the trumpets of doom sound, some smart engineer or geologist comes up with a new way to extract more oil. I don't see any reason why this trend will suddenly stop this time, we still have lots of tar sands, deep oil, and shale oil that have not been touched. Please note that I am not expressing a political view on the social correctness of these options just the technical aspects. Even more impressive are the reported quantities of frozen methane hydrate clathrates on the ocean floor that would likely be fairly easy to extract. Some estimates are that there is more than 10 times the amount of energy stored there than in all the oil we have ever used. Obviously none of these fossil fuels address the CO2 generation concerns that many people have.
Wind, solar, hydro and even nuclear power all have their places and I hope their niches keep growing as the technology improves, but nothing comes close to competing with chemical energy as a cheap, portable, high density source of energy. One just needs to look at biology to see the truth of this; plants fix the suns energy into chemical forms that then cascade through the food chain ever evolving into more complex forms. How cool is that!
Thanks for helping me stretch my brain each week! Thus ends "This Week in Chemical Engineering"!
EH&S Manager, LSG
How about phage fossils?
Now that we know that phages have an apparently symbiotic relationship with mammals, is it too farfetched to imagine that some phages were once incorporated into - and expressed - in metazoa?
One line of research might be a search for such fossil sequences in the genomic databases that already exist. Perhaps someone is already doing it?
The reference to salicylates in my last email was prompted by Dr. Schacter's comment that methyl salicylate reminded him of acetylsalicylic acid, and to show how the acetyl group was important in the clinical effects of the latter.
Thanks for such thought-provoking and paradigm-shifting discussions!
I was intrigued by the comments on the antimicrobial effects of Cu, having used silver sutures in contaminated cases in the 60s. It struck me that an easy and cheap test would be to copper plate [which uses very little ] the door .faucet and toilet handles in an ICU and see if the Hospital acquired infections changed. This is fairly low tech requiring a screwdriver, a auto battery and some CuSO4 [root killer.] or could be done by many firms who do it commercially. Thanks for the really good podcast
Hi, I am a big fan of your great education/entertaining programs. I would like to suggest Vincent to invite experts in antimicrobial resistant bacteria in his newly launched TWiM program. This problem is very serious and thus the WHO themed their day with the antibiotic resistance issue. Likewise the CDC chose a week on September last year to address the devastating health related issue. Inviting Experts and pioneers in this field will highlight and explain queries.
I recall hearing about a bacterial contamination of cantaloupe years ago, which is when I started to wash them in soapy water before slicing.
Of course, I try to follow the rules. We actually cut meat products on the counter between the stove and sink, and the rest on the kitchen island to prevent cross contamination. Often I will prep for cooking by cutting the veggies on a dishwasher proof cutting board on the island, put them in bowls and set aside. Then move the cutting board to the counter, cut the meat and either put it in the bowl or the pan. Then put the cutting board in the dishwasher, set the knife to be hand washed next o the sink. Then cooking is quicker because I have everything ready, using a food safe mise a place.
The other day I was watching an old cooking show with Julia Child. I just about gagged when I saw the other chef she was working with put an herb stuffing between the breast and skin, and then reach over to pick up salt from a bowl with his fingers without washing his hands or using a spoon!
I have a TWiM idea from watching cooking shows, especially one that showed all of the varieties of salt: pink salt, which gets its color from an extremophile bacteria (a tidbit I want to share at the local upscale kitchen store when I get a chance!). Oh, cool, just checking the halophile wiki, I learned they are essential for some fermented foods like soy sauce, sauerkraut, anchovies, etc: http://en.wikipedia.org/wiki/Halophile
First: Thanks for making a career of TWI-Podcasts! A great service and an entertaining and educational format.
In TWIM #1 you reviewed the antibacterial properties of copper and it’s potential for control of MRSA compared to other methods being pursued. While the science of antibiotic mechanisms is fascinating, we may be blinded by the technology instead of understanding the logic of the problem. The references below are a few of the many showing (not surprisingly) that the people who spend their days around hospitals are the reservoirs for MRSA. Anywhere from 6% to 21% of healthcare workers (in this case anyone working in a hospital) have been shown to carry MRSA in their nares. This is higher than the rate in incoming patients. Remove the reservoirs and control is much easier!
While we are great at technical solutions, we are close to powerless at social, particularly politically social solutions. It would seem that a major reduction could be achieved through regular testing of healthcare workers (<$10 & <24hrs) to identify carriers, their removal from patient care, treatment with mupirocin (I believe topically) and return to patient care. Hospitals object to the cost of testing although as carriage rates decrease so would costs. Unions object to the potential unpaid removal from work during treatment as well as the treatment cost.
Doctors generally refuse to be tested, even for hepatitis or HIV. Despite the enormous monetary cost of MRSA to hospitals and to our overall healthcare system, plus the unnecessary deaths, our society insists that “magic bullet” pharmaceuticals and antibacterial hardware are the only way to solve this problem. Pogo had it right about “the enemy”.
Thanks again for the education and entertainment. Vincent and your TWIV, TWIP, TWIM teams are an exceptionally bright spot in scientific education!
Prevalence and Characteristics of Staphylococcus aureus Colonization among Healthcare Professionals in an Urban Teaching Hospital infection control and hospital epidemiology june 2010, vol. 31, no. 6
Prevalence of Staphylococcus aureus Nasal Colonization in Emergency Department Personnel Annals of Emergency Medicine, Volume 52, No. 5: November 2008, p.525 – 531
Incidence and Prevalence of Methicillin-Resistant Staphylococcus aureus Nasal Carriage Among Healthcare Workers in Geriatric Departments: Relevance to Preventive Measures INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY Vol. 24 No. 6, p.456-458
How Often Do Asymptomatic Healthcare Workers Cause Methicillin-Resistant Staphylococcus aureus Outbreaks? A Systematic Evaluation infection control and hospital epidemiology october 2006, vol. 27, no. 10
Successful Control of Widespread Methicillin-Resistant Staphylococcus aureus Colonization and Infection in a Large Teaching Hospital in The Netherlands infection control and hospital epidemiology august 2007, vol. 28, no. 8
This is the greatest TWiM ever. (#58)
I've always been interested in evolutionary complexity and for the last seven years or so I've been unable to think of evolution in terms of traditional survival of the fittest on an individual level--at first it just seemed too simple. But through these years of rumination on the subject often provoked by a science story in the news (or on a science-ey show (radio-lab, I'm looking at you--I love you but I'm looking at you)) that approaches evolution through the perspective of survivability of the individual as opposed to survivability of a complex ecosystem of interactive life-ey things and then failing to see obvious conclusions because of this narrow view, I'm nearly unable to conceive of the idea of an 'individual' organism having any meaningful impact on (even short term) evolution.
This episode of TWiM starts with Elio giving a very masterful explanation of the paper: Underground Mycelial Networks Carry Warning Signals to Plants. His gentle and thorough description makes me long for an opportunity to attend a lecture class with him. He is truly a masterful lecturer with managed digressions that are interesting and make it clear that he is processing the information further as he speaks making the reality of hearing a brain (or brain-biome) at the top of its performance and with such great depth of knowledge really tangible and exciting.
Of course the content of that paper is also remarkable and Vincent and Michael do spectacular jobs of interjecting and referring to relevant information as well as questioning things where it's due. The dynamic in this episode is really great.
Then to top it off the second paper about the Brain Microbiome is as amazing as the first paper. During the discussion of this paper one can really feel the excitement and disbelief from the trio as they consider the implications of how this can change our understanding of the brain. I'd like to add that I wasn't at all surprised by the fact that our brain has it's own microbiome (I actually was a little surprised that it wasn't a foregone conclusion).
This episode was truly enlightening and highly entertaining. I was so riveted to the entire thing--I felt like that's what witnessing the moon-landing for the first time must have been like--it's real discovery and really cool.
So thanks TWiM guys and keep up the work. I don't care if you don't produce an episode every week, just get them out occasionally because this is important stuff that people need to know.
Finally: I'd just like to throw this out there: I'm at the point now where when I seriously think of how things evolve, all pieces of a system have to be taken into an account. It's easiest for me to grok if I consider a physical space and all of the life and non-life inside it and then also to consider a significant time-period that must include thousands if not millions of generations of changes and co-dependencies that have developed and curated. I know life has evolved to evolve (I can give a number of examples of that) and I know life has evolved to evolve to evolve (I even have a couple of that) the next level isn't easy to see but I guarantee it's there and it probably recurrses an innumerable amount of times in ways that are both simple and complex and we just have yet to understand.
I'll leave you with this: Despite the appearance of randomness guiding the evolution of the beaks on Darwin's finches I promise that the beaks of those finches have evolved to have high-rates of randomization and likely even environmental influences that either magnify or reduce the amount of randomness that's displayed--after all, evolving a trait that helps them evolve will increase survivability and that's the meaning of life.
Thanks for the great TWiM!
Hi Vincent et al
I have been listening to TWiM for several weeks after moving to a new job that requires me to undertake a one-hour drive to work. I enjoy the show very much: in fact, I enjoy it so much that I am thinking of setting up a similar podcast this side of the Atlantic.
But this is not the reason I am writing.
I listened to your discussion of the brain microbiome this week. And, even though I note the question mark in your title after the term "brain microbiome", I think you were not skeptical enough.
OK, I admit that the paper presents an internally consistent story. But they don't report trying to grow the bacteria. And, rather than one organism, they would have us believe there is a kitchen sink's worth of microbes in these brains. Remember when we look at most sterile samples, we assume that a mixed growth equals contamination! And alpha-proteobacteria are common contaminants of water supplies, even in the International Space Station! http://www.ncbi.nlm.nih.gov/pubmed/16364606
But more generally, as Carl Sagan put it, extraordinary claims require extraordinary evidence! And IMHO the evidence presented here is not compelling enough to overturn over a century of microbiology and histopathology. If these bacteria are really there, would we not have seen them already? If we look at precedents, we could be looking at the next Helicobacter pylori and these authors are going to be Nobel laureates. But I think a more plausible precedent is XMRV or arsenic life, both of which turned out to be nonsense. Go take a look at Ioniddis' paper "Why most published research findings are false" http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0020124
Only time and attempts at independent replication will tell what is going on here. But I am prepared to wager that in ten years time this paper will have been discredited or forgotten.
Keep up the good work!
Professor Mark Pallen
Professor of Microbial Genomics and
Head of the Division of Microbiology and Infection
Warwick Medical School
University of Warwick
TWiM #58: The brain microbiome
The brain microbiome would have been outright heresy in the latter 1960s when I had medical microbiology in med school. Today's facts are stranger than the wildest imaginable fiction of a bygone era.
Both papers go to show the enormously complex interconnectedness of all things.
The idea of signals transmitted through a network of mycorrhiza suggests a slow intelligence with signals acting over hours or days instead of milliseconds as is the case with neurones. An awareness associated with such an intelligence would have little commonality with what is familiar to us. Even though chemical inputs equivalent to smell and taste, mechanical inputs equivalent to hearing and touch, and light inputs equivalent to vision could affect such a system, they would be so different that it may be impossible to form a concept of such awareness.
It is to be noted that in Eastern traditions, awareness is consciousness with content; appropriately organised complex configurations of matter and energy will manifest awareness just as non-magnetic pieces of iron in a magnetic field will manifest magnetism.
Methyl salicylate is present in large quantities in oil of wintergreen: even a teaspoon if ingested can cause severe salicylate toxicity. Aspirin is acetylsalicylic acid. It is less irritating and less toxic than methyl salicylate. The intrinsic stickiness of blood platelets depends on platelet cyclooxygenase, which is inactivated by acetylation by acetylsalicylic acid. A single tablet of aspirin will inactivate the platelet cyclooxygenase in all the platelets. It also affects the other blood cells, but unlike platelets, they regenerate their cyclooxygenase. Platelets have to be replaced by newly formed platelets. Since platelets have a lifespan of seven days, a tablet of aspirin is good for two to three days.
Hi Vincent et al!
I was listening to the TWIM episode #55: In the copper room, about bringing down hospital infections.
You mentioned two things as being the main problem. The first one was high occupancy rate.
Quote from the episode:
"The second and this is probably the most important reason, is that you can't tell that things are dirty - from a microbial perspective. And we don't routinely survey the area to ask what is the microbial load"
I am thinking, what if the bacterias could somehow be made visible. It would be real cool if material could be engineered to change color to show if there is a high level of bacterias and other pathogens, to alert that a surface needs to be cleaned. It doesn't necessarily need to be visible to the naked eye, but just if some special lights got shined on it. I have no idea if it will be possible, I just liked the thought.
As you say, the main problem about hospital infections, is that the problem is invisible. And if things are invisible to our eyes, it is harder to shine mental light on it, because we don't directly get reminded about it on a daily basis. Hereby my wish for "bug luminol" is passed on.
I really enjoyed the discussion of PULs on the most recent episode of TWIM. I had a minor correction with respect to the discussion of the connection between diet and IBD. During the discussion, one of you talked about a progression from ulcerative colitis to Crohn’s disease. In fact – these diseases, although categorized as inflammatory bowel diseases, have fairly different (albeit poorly understood) etiologies. UC is generally restricted to the colon while CD can be localized anywhere between the mouth and anus. Also, IBD patients generally have one or the other – but not both. They don’t really progress from one to the other in any individual patient.
Keep up the great discussions of the wonderful world of bacteria and human health.
Joseph McPhee Ph.D.
Michael G. DeGroote Post-Doctoral Fellow
McMaster University, Department of Biochemistry and Biomedical Sciences
Most interesting talks on copper in disease transmission reduction environments. How about silver?
I possess several pairs of army socks (literally), a percentage of the fibers (3%? 12%?) woven into which are microcoated (or embedded, or something) with silver. The purpose is suppression of undesirable (potentially mission-defeating) microbes.
I believe they are effective. I believe there is some literature on the efficacy of silver, and rationale for its selection over copper. I think I've seen some ventures at explaining the mechanism, but I don't have the stuff in my head and can't go looking. Worth observing that they seem to enable wearing socks for more than one day without getting stinky.
Interesting aside: I went looking for army socks after hearing an ER doc describe managing the pain of 36 hours on his feet by wearing wool army socks. His belief was that the wool provided a sufficient secondary cushion to dampen the impact of walking all day on concrete floors (which have no give, relative to asphalt or whatever). My feet have plagued me with easily acquired aching, so I went looking. The socks, combined with placebo action, seemed to provide noteworthy reduction of discomfort. As to heat objections, which seem culturally common among us, he reported year-round use without problem. I found them entirely comfortable, probably downright insulating, when worn in Fresno summers under boots, and certainly no worse than conventional or lightweight socks, so I ratify his year-round prescription, since they're pleasantly warm in winter too. (pause for breath) Not itchy either, in this formulation, to the contrary of old complaints about wool.
Anyway, recently I went to the military/police gear store for more socks. I was disappointed not to find the familiar thick wool, but did decide to try the new blends. Some were thinner wool blends, and others blend cotton with synthetics such as lycra, I guess. I sprung for some, and was so pleased with the (quite inexpensive, relative to Walmart work socks) performance of the silver blended ones that I went back for more. This model is near knee-high, so has the benefits (for those to whom it matters) of compression as well, potentially reducing edema and whatnot.
Anyway, whatever. Much enjoy the show(s), encountered on internet radio Science360 (which you might mention sometime). Hope you find the bit on silver vs. copper interesting. Maybe it suggests a further look along the table of elements.
Michael Schmidt's enthusiasm and passion for all science is always wonderful to listen to. Hearing him discuss his own work was even more of a treat. Congrats to him and his collaborators on their great work telling a fantastic and relevant story!
Dear TwiM podcasters,
Thanks for another great episode! Each TwiM/P/V episode you do brings me so much inspiration and I wait impatiently for another one to come. Usually, though after each episode I have more questions than answers, which I think is great because that what science is based on, like Feynman said: science is expanding frontier of ignorance the more we know the more there is to answer.
Regarding the last episode, I always find these phage-bacteria arms races very fascinating; they are a perfect example of how evolution can work in short period of time.
On last episode it was mentioned that CRISPR/Cas system takes up DNA randomly irrespectively of whether that would come from a "helpful" or a "bad" phage. If the process is random then would it also take up DNA that was acquired through transformation or transduction?
Can Cas somehow distinguish if it's a phage DNA or not?
Is it maybe specific for some kind of base modification like hydroxymethylcytosine that are found often in phage DNA, or does DNA sequence has to be of certain size, or maybe the DNA has to be either linear or circular (but if circular then why the phage induced resistance islands do not get accidentally incorporated into the CRISPR locus)?
Can one do a BLAST search to find if the spacer sequences in CRISPR locus are exclusively from phages or sequences from other sources are also present? I know that it would probably be of no use to incorporate random sequences into the system but then I presume that there has to be a mechanisms for distinguishing where the DNA comes from?
Andy Camilli writes:
As far as we know, the capture system is random. The phage CRISPR/Cas even occasionally captures a piece of its own genome, which is a big mistake, because it will then degrade its own DNA! What we find in these instances, is that points mutations have arisen, either in the spacer or in the target in the phage genome, that abrogate hybridization of the crRNA and thus degradation is prevented. Presumably, these mutations are selected for rather quickly.
Re: ASM Discussion - I wonder how much journal's biases against publishing negative results contributes to misconduct.
Hi TWiM team
I hope you were able to see the Google tribute to Julius Richard Petri:
Not sure about the streaking technique shown in the doodle though.
This is old on uTube, but new to me. Can I suggest it as a listener's pick. Surely a similar dance be created from all the fields your podcasts represent!
I heard about the dance from class 129 by Dr. Gerald Cizadlo. His engaging manner is somewhat like yours and just this year he began offering an online version of this class, Bio 3020.
Dr Schmidt, on TWIM 35, after the 2012 ASM conference, you mentioned use of the Quartzy networking cards at many poster sessions. Did you see the cards used this year, were they more prevalent (3,300 used last year), and were poster sessions as plentiful as last year? Have the cards stimulated any competitors?
PS: TWIM 56 at the ASM was awesome, just awesome! It's like listening in on a discussion between Einstein, Edison and Feynman.
Dear TWiM team
I see that the Oregon Senate approved a bill establishing brewer's yeast, Saccharomyces cerevisiae as Oregon's official state microbe.
I can understand the popularity of brewers yeast, what other microbes do you think could be chosen as official state microbes?
I think food and drink related microbes would be favoured by most people so various species of Lactobacillus may be popular.
On that subject I see that there is a paper on microbiological profiles of ŞALGAM, the Turkish lactic acid fermented turnip and black carrot drink:
It is a popular drink in Turkey but rather an acquired taste for those unfamiliar with it.
My Dad sent me this article after I sent him a link to your episode on biospeleology.
It's about biofilm sheets in underwater caves under the Australian desert that glitter in the torchlight due to calcite crystals that are formed in the biofilm.
Here's a link to a Maker site that discusses how to do a 3D print of your brain.
Dear Vincent and his fellow TWIMsters,
Hello, my name is Daniel, I am a first year graduate student at Michigan State. I would like to thank you all for making a great podcast. I recently caught up to the most current episodes by having my own TWIM marathon during my 6 hour drive home to Wisconsin over the holiday break.
I'm not sure where to begin explaining how much I value this podcast. As someone who cares for outreach, education, research and in general having a good time with science, you folks are first rate. This series has been accessible all the way through for grandparents to grad students. When things maybe get a little to verbose you have done well to bring it back down. But you also make it just intriguing enough to hold the attention of the grad student in me. I have even been known to actually follow along in the articles. :) And of course I have had a great time learning and exercising my mind while listening in. You guys went even further to impress me with the discussion of Jo Handelsman's research last time, it gave me lots to think about for the future. Please keep up the great show!
I also wanted to share a paper with you all that I thought about after listening to TWIM #47: Resistance On the Surface. Michael said something that made me remember it during his discussion of the paper in reguard to conjugation. He said, "It's having sex wicked fast!", and you guys went on to exclaim about how fast conjugation was happening.
In this Science paper by Babic et al. in 2008 they are using a very beautifully designed experiment to visualize DNA transfer by conjugation, and it agrees... wicked fast! I suggest the supplemental videos. The experiment involves a recipient strain that is Dam- or defective for DNA methylation by Dam methylase, and which has a SecA-YFP fusion . (Note by MGS--- it is not SecA...it is SeqA ) SecA being (correct me if I'm wrong) the protein that keeps replication from occurring when hemi-methylated DNA is present in the cell. Effectively keeping multiple rounds of replication from occurring at once since newly replicated DNA has not yet had the time to be methylated on the new strand.
The result of this recipient cell is no DNA methylation, or SecA-YFP binding and diffuse fluorescence. The donor cell, however, has Dam methylase and will donate methylated DNA during conjugation. When this happens, the donor DNA will become hemi-methylated upon replication allowing SecA-YFP to bind and show a localized fluorescence to that DNA. This means we can watch it happen at a single cell level! They do show a rather surprisingly fast transfer of DNA, as well as make other neat conclusions in the paper which include the use of a red fluorescent gene on the donor DNA to measure expression of conjugated DNA, but the e-mail is long enough already. It's not a bad read, and I hope my explanation was TWIMable enough.
Thank you for providing a wonderful supplement to my microbiology education, and Happy New Year,
Here is the link to the article.
I am listening to eps 49 and heard the mention of biodynamic vineyards. Biodynamic farming is similar to organic farming, with the addition of such things as burying dung in the horn of a bull and sprinkling magical powders around the farm. Take a look, it's quite entertaining total nonsense.
Clark writes: [this email was read on TWiM #56 but not published]
I'm slowly getting caught up with TWIM after Christmas and just listened to episode 43. I came away very confused and I hope you can clarify something for me.
I understood that the major problem with using phages for disease was due to FDA regulation. However when speaking about acne you suggested that there were ways for the FDA to approve them as a type of drug treatment. If this is the case, then why on earth aren't they being used to treat anti-biotic resistant TB or other such diseases?
If you could do a show on the interplay between the FDA, government regulation and commercial use of phages to control disease I'd be quite excited. I confess I get confused when hearing about the problem of antibiotic resistance on the one hand and phages on the other.
(also, I love your podcast, and in the future when I write a longer letter I'll explain more why I love the podcast)
Long time listener, 2nd time emailer. ID doc. Mostly i treat HIV patients and hospital acquired infections. You've talked a lot about copper as an antimicrobial and that ancient civilizations might have used it to reduce war wound infections. I'm not aware of it's use in modern wound care. However silver based products are used a lot in wound care and on catheters, etc. I would be interested in understanding the antimicrobial properties of silver, and in why copper is not used topically if it is so effective at killing bacteria. Thanks.
Thought Michael Schmidt might like this reference. It is to a Canadian podcast called Quirks and Quarks.
The reference is: http://www.cbc.ca/quirks/episode/2013/01/19/january-19-2013/index.html#3
In this episode, they discuss a paper by Jayne Danska talking about diabetes in nonobese mice. The outcome of diabetes can be changed by changing the microbiome in young mice. It reminded me of Michael' s thinking on how the microbiome might come into play in the future.
I found it very interesting after hearing the TWIM episode.
The paper appeared in Science.
Hello to the TWIM team and guest(s) I have written to TWIV but this is my first letter to TWIM. I was recently watching a documentary about
how beer was the driving force behind beginning agriculture and numerous other massive human accomplishments and the documentary
mentioned skeletons from 500 BCE containing tetracycline. My first reaction to this was where is my computer I have to find this article.
Well I did find that the orgiginal discovery in the late 1980's was meant with extreme skeptacism (not surprisingly) but then I also found
an article from 2010 with more data to back up the claim. Now, I am not one to interpret Mass Spec data very well but would love to hear
what the TWIM team thinks of this. Here is a link I found to the article in the American Journal of Physical Anthropology http://onlinelibrary.wiley.com/doi/10.1002/ajpa.21340/full
Thanks to all for the wonderful podcast,
Alaric the Great
One would expect that Italians would be familiar with Alaric the Great.
Alaric I (Gothic: Alareiks; 370 – 410) was the King of the Visigoths from 395–410. Alaric is most famous for his sack of Rome in 410, which marked a decisive event in the decline of the Roman Empire.
Great HAI work! I'm not through it yet. I want to question, though, whether fomites are really important in influenza transmission. I don't think so.
Wink Weinberg (ID)
[flu can transmit by fomites in guinea pigs: http://jid.oxfordjournals.org/content/199/6/858.full ]
I'm a second year microbiology undergraduate and I very much enjoy all of your podcasts; they are a great pleasure to listen and I wait for the new ones every week.
Since the last episode was all about copper I would thought it would be appropriate to point out this paper published in Nature in 1984- "Why whip egg whites in copper bowls?".
Who would have thought that a paper about copper bowls and cooking could've been published in Nature :)
I also think that this paper could be shortly discussed one one of the podcast: traditional mutational study but seems quite perspective- what do you think?
"Identification of Salmonella Pathogenicity Island-2 Type III Secretion System Effectors Involved in Intramacrophage Replication of S. enterica Serovar Typhimurium: Implications for Rational Vaccine Design"
Thanks again for your podcasts and keep them coming!
I formerly developed a oxygen radical (not ozone) surgical instrument sterilizer so have dealt with the difficulties of disinfection and HSAs. Successfully sold to Stryker Instruments.
You mention the problem of economically obtaining copper hardware due to the pervasive use of plastic. If you think about our common household plumbing fixtures, it is clear that plastic can be plated with many metals and shipped around the world by air.
Plastic plumbing fixtures are typically plated with electroless copper followed by nickel then chromium. Air shipment is not an issue so your former plating vendor simply did not have the technique/knowledge to give you a sturdy uncontaminated injection molded part. I was involved on the development of many of these processes in the 70s. Rhom & Haas is still a major supplier of plating solutions and methods.
Similarly, any metal part can be easily plated in whole or in part with copper or copper alloys by the OEM. A quick swipe with ammonia will remove finger oils which could allow bacterial film growth and will expose fresh copper surface.
Despite all this antimicrobial furor, I look forward to the "omic" studies identifying how many HSAs are from the environment vs. the patient. Microbiome studies seem to be showing that microbial diversity balance rather than eradication is often the key mechanism of maintaining health.
Thanks to all you TWIV, TWIP & TWIMmers for creating and maintaining one of the most educational, accessible and plain old FUN scientific forums ever!
I was amazed to see note 13 in the caption for this article out of Apr/May 2013 AARP magazine about use of copper alloys on frequently touched surfaces. Copy of that page attached as a PDF.
I love your show, especially the recent episode about the use of copper in hospitals.
I am a nursing student at University of Nevada, Las Vegas and I am always cleaning patient bed rails and call lights. Patient hands are not the only thing that comes in contact with bed rails. The rail is also frequently used by nurses to temporarily hang pieces of tape while inserting an IV or changing a dressing. The tape, with all its germs attached, is removed from the bed rail and taped onto the patient's skin in close proximity to an open wound.
A future study might include the pathogens transmitted by stethescopes. People are pretty good about washing their hands, but I never see anyone cleaning their stethescope. It is my very unscientific, empiric observation that the stethescope is one of the biggest fomites in any hospital. I have seen copper writing pens for sale, but I am not aware of anyone who makes a copper coated stethescope.
Another possible fomite is employee badges, they come in contact with our patients when we lean over them (I stuff mine in my pocket so it does not contact the patient.)
Hello TWIM friends!
Jennie here - a long time fan of TWIV, then TWIP and of course happily learning from your great TWIM podcasts.
Thanks to Michael Schmidt's fascinating discussions - including those regarding copper and microbes - which have really got me thinking. (Copper's natural anti-microbial activity can lower nosocomial infections by decreasing pathogens on hospital surfaces. To plagiarize Mr. Schmidt's own site at the Department of Microbiology and Immunology at the Medical University of South Carolina: "The 4th leading cause of death in the United States, behind heart disease, cancer and stroke, is Hospital Acquired Infections (HAI) where approximately five percent of the patients admitted to US hospitals will acquire an infection. Very little is known of what fraction of these infections result from a microbial contribution obtained from objects present in the built environment."
It appears that copper does have a contribution to make - and I can imagine that there will be some formidable costs in changing surfaces from plastic & alloy & steel to copper.
To decrease costs of switching to copper surfaces for commonly touched fomites (microbe carrying objects) like IV poles and steel hospital infant bassinet units - you're talking to an OB nurse here - I began to think about a 6th or 7th grade experiment we did in school when I was a youngster with copper plating.
With that in mind - I did a quick search for a video on copper plating on Youtube and found this video - which just gives you a peek at how easy it can be to copper plate existing metal surfaces. I'm wondering if this could potentially be a cost saving application for some institutions. Will it be necessary for institutions to re-purchase when perhaps they could resurface?
Of course, I hope that you realize how very much your team has done and is doing to increase our understanding of the tiny denizens without and within us. What an adventure!
Special thanks to you Vincent for sparking greater excitement and transparency in science. Thanks to Jo Handelsman - soil microbes are so vital and so unknown - thanks for the recent apple orchard soil discussion and for her deeply appreciated advocacy for women - Yay! Of course Elio Schecter and Stanley Maloy - fantastic!
Yours with warmest regards
Jennie BSN RN
“In the world through which I travel, I am endlessly creating myself.”Frantz Fanon
Saw this media release from the Australian Institute of Marine Science about researchers isolating a combination of probiotic bacteria to assist in the prevention of Vibrio infections of spiny lobsters in aquaculture and thought of TWiM.
Keep up the good work,
12 December 2012
Winning combination of bacteria found to combat deadly marine pathogen
Research conducted at the Australian Institute of Marine Science (AIMS) has delivered promising results in combating Vibrio owensii - a bacterium that is responsible for mass mortalities of cultured ornate spiny lobster larvae.
The high commercial value of the ornate spiny lobster (Panulirus ornatus) means it has the potential to be an important product of the Australian aquaculture industry. However, nutritional deficits and bacterial disease during the long larval phase of the species makes captive rearing difficult.
Scientists from AIMS and the University of New England (UNE) have been able to isolate a large number of bacterial cultures – or probiotic candidates – from wild lobster larvae and their natural prey items, and from the lobster aquaculture system at AIMS in Townsville. After successive tests, they found that a combination of two probiotic bacteria, referred to as PP05 and PP107, provided the most effective protection against the pathogen Vibrio owensii, enhancing survival of the larvae by as much as 80 per cent.
AIMS Research Scientist, Dr Lone Høj, who led the project, said “Our work has uncovered a winning combination of “good” bacteria that appear to dramatically improve larval survival. In a further study we looked at how and why these two bacteria were so effective when working together against Vibrio owensii.”
UNE PhD student Evan Goulden said “This research highlights the value of identifying biocontrol agents that are able to intercept the infection cycle of a serious aquaculture pathogen, as such the study represents a milestone in proving the value of using probiotic mixes to prevent microbial diseases.”
“Disease management is critical in food production systems and this is particularly true for seafood produced in aquaculture systems. The development of alternatives to the antibiotics currently used in such systems is becoming a national priority in countries around the world” says AIMS Principal Research Scientist, Dr Mike Hall.
‘Identification of an Antagonistic Probiotic Combination Protecting Ornate Spiny Lobster (Panulirus ornatus) Larvae against Vibrio owensii Infection’ is published in PLOS One: http://dx.plos.org/10.1371/journal.pone.0039667
‘Probiont niche specialization contributes to additive protection against Vibrio owensii in spiny lobster larvae’ is published in Environmental Microbiology Reports:http://onlinelibrary.wiley.com/doi/10.1111/1758-2229.12007/abstract
The authors are Evan Goulden (AIMS/UNE), Mike Hall (AIMS), Lily Pereg (UNE), Brett Baillie (AIMS), and Lone Høj (AIMS).
Dear TWiM team,
Since the topic of patent law came up on episode 48 I wanted to add my two cents. Six months ago, I switched to a career in patent law after 12 years as a bench Virologist. I have found this job to be very challenging and rewarding, and I'm currently experiencing a rather steep learning curve. However, the reason I'm writing is because I did want to mention that one does not have to be a lawyer, or even plan to go to law school to practice patent law. Many firms are willing to hire PhDs with no prior experience in patent law as science advisors or patent agents and train them on the job. The reason they're willing to do that, is because of their extensive background in science, which is imperative in this particular legal field. In our firm, all of our six science advisors have PhDs, and three of our five attorneys do as well. Although learning the law is pretty difficult, in my opinion, it is much easier to learn the law on the job than it would be to learn the science, and I have tremendous respect for the two attorneys in our firm who do telephone directory ireland reverse phone lookup not have PhDs, because they seem to be so well versed in the science as well. I recently asked one of our partners (who has a PhD) how much of what he learned in law school he has actually applied to this particular job, and he said “zero”! On the other hand, attorneys do make a lot more money, and there are certain things that they can do that a patent agent can't do, but my point is that a PhD is more than enough to have a rewarding career in patent law. Personally, I have absolutely no desire to go to law school!
Thanks again for continuing to provide so many different stimulating and thought-provoking topics.
Post-Sandy seems an appropriate time for a TWIM devoted to mold since the storm generated many opportunities to deal with it? I'm also battling it in my ventilation ducts to the extent that we replaced all the supply lines beneath the house and some of the returns in the attic, and installed an electrostatic filter upstream from a HEPA-type filter, plus a UV light by the heating/cooling coils. Over the years I've inspected the ductwork for integrity and cleanliness and just didn't think we had conditions that allowed mold growth until we found 7 of 23 supply lines each of which that looked inside like they were spray-painted with black primer over a good many feet. Meanwhile outside we've been unable to prevent black mildew from growing in playing-card patches on treated wood coated with mildewicide-infused stain and exposed to sunlight about eight hours a day.
I bought five mold collection kits containing petri dishes and growth medium just before detecting the seven register lines. At first they seemed a good idea to apply now and perhaps in the spring or summer, but then I found this site with considerable mold information that seems reputable. My interpretation of what the site says is that conditions and materials contributing to mold must be removed to fix problem. While removing bad ductwork helps I don't think it corrects the condition problem(s). The site says sprays and chemicals don't work! And sampling with my kits followed by lab work to identify mold types won't tell if harmful mold is at levels requiring action. In addition, unless you use laboratory grade filtration, it won't reduce the presence of mold to livable levels, and UV radiation is only good directly under the light, not particulates flying by. Electrostatic filters apparently only remove a small amount of mold-related material as do good filters, but not all and probably not enough for sensitive people, plus they work best if the ventilation fan runs continuously. Much harmful mold material is too heavy to get sucked into any ventilation system, anyway, and is only removed by vacuuming often, mopping and washing fabrics. Finally, only an expensive, trained environmental specialist can do a good evaluation, prescribe corrective action and determine if that action has been effective. This site shows we have just one such specialist in my state,Virginia, about 300 miles away.
Do you folks agree our best approach would be to napalm the house and replace it with a stainless steel cube, or wear environmental protection suits, or move to who-knows-where....? Of course the guys doing all the ventilation work used no respiration protection, but then they were all in their 20's and 30's versus our 70's.
So what's going on with mold nowadays, anyway? Is there more of it? I've no problem understanding why you need to remove wet and moldy plasterboard and carpeting, but have seen home shows where mildew-stained woodwork behind the wallboard is sprayed with something which they implied would fix the problem. You're the only really reliable source of complete and competent knowledge on the topic, so many of your listeners should appreciate your comments.
When do we get to hear the results of Michael's experiments and interventions with copper and microbes?. The suspense is killing me, or did I miss it? Please continue your marvelous podcasts.
Vincent, TWIV, TWIP amd TWIM are wonderful for a retired person like myself. I worked in IT, but started out with a biology undergrad that instilled my interest in biology many years ago. I always look forward to the next podcast. I have discovered that dinner conversation about TWIP is not as well received as a discussion about my latest project from the course I am taking at the College of Craft and Design. You will enjoy this
Hello, I am a big fan of all your podcasts. Copper is used as an anti- parasite agent in marine aquariums. It is toxic to all invertebrates and is deadly to them so it is used in fish only quarantines. This is perhaps why there is a concern about copper in the marine environments as it will not only kill microbes but snails, crabs, squid ect.
Hey guys, love your show so far. I have a few thoughts on the last episode concerning B. anthracis, particularly the lack of variance in anthracis. I found it very interesting to learn that spore-forming species have characteristically more genetic variance than non-spore formers. Does the lack of genetic variance have something to do with the fact that anthracis forms endospores, and not spores in a fungal sense? Fungal spores are easily released into the air for dissemination, while anthracis endospores seem fit to persist in the soil and dead bodies of infected animals. Do fungal spores show more genetic variation in a strategy to survive in a more diverse environment? Maybe Bacillus anthracis spores are less inclined to disseminate throughout the environment because the cattle and sheep they typically infect die within a few days and are generally confined to the field of the farmer who owns them.
Thanks, keep up the great work!
Hello Professors and Expert,
I am hoping for an upcoming episode on resistant bacteria or upcoming antibiotic drugs in the pipeline?
Your fellow cytogenetics friend,
Northwestern Memorial Hospital
I love this new podcast, and I especially enjoyed this episode. This is a nice example of both the strength and weaknesses of scientific forensic evidence, I think--you can get useful information out of it (the anthrax strains in Ivins' lab are very likely the source of the anthrax used in the attacks), but not the critical questions you care about (was it Ivins who made the spores and mailed them, or was it someone else; if it was Ivins, did he have any help?) I suppose we will never really know for sure, since Ivins is dead and the FBI's investigation is over.
Assuming Ivins was the attacker, this raises an interesting point with respect to the threat of bioterrorism: Ivins was a US government biological weapons researcher, working on defending against a potential attack. He had expertise, equipment, training, supplies, and access to information at a level that it's hard to imagine any other terrorist having. And yet, his total body count Online Pokies was quite small, as these things go. Some nut with a gun in a crowded mall might manage to kill about that many people! The attacks were quite effective at spreading terror, especially among people at the top of government and media, but they weren't actually all that deadly.
My question is, does this suggest that bioterrorism is overblown as a threat? Or is it just that this attack wasn't targeted at killing large numbers of people, or that we got lucky and things might have ended up far worse? My understanding is that anthrax isn't really much of a risk to spread after it has been used, and once you know people have been exposed you can give them antibiotics that will save them, so the attacks were probably self-limiting. Maybe the situation would have been different, had the attacker had access to a different agent?
This link, http://www.thermus.org/e_index.htm , is a good source of information about Japanese activities, has links to seminars, abstracts, software information that's being used, project background, 3D visualizations and progress reports, all in English. Might be of interest to listeners who wish to see what is going on in East.
There is an effort underway to ban Triclosan and I would like to hear TWIM discuss the risk / benefit to using anti-bacterial agents, particularly their widespread use in consumer products. Any info on how useful Triclosan is whether we'd miss it if was banned would be appreciated.
Kerry (Loyal laymen listener toTWIV, TWIP and now TWIM)
I just wanted to comment on your short discussion of nomenclature for Salmonella. Panama is not a serovar of enteriditis. Salmonella is currently recognized as having two distinct species, Salmonella enterica and Salmonella bongori. Salmonella enterica has 6 subspecies, of which subspecies enterica is the most clinically relevant. Within Salmonella enterica subspecies enterica, there are many serovars, of which Panama is a representative. Enteriditis is also a serovar of Salmonella enterica subsp. enterica. Therefore the proper name for this outbreak strain would be Salmonella enterica subspecies enterica serovar Panama. Whenever Salmonella Panama is used, or Salmonella Enteriditis, it implies that the serovar is a member of the enterica subspecies enterica family.
Vincent and everyone else on TWiM, I have throughly enjoyed these podcasts, they are perfect entertainment while I am stuck spinning down large culture volumes. I especially enjoy the format, focusing on just a few papers/topics really allows for in depth discussion.
Please continue your coverage on all the human microbiome work, it is a fascinating topic. As a undergrad at Cornell I was lucky enough to take the first offering of a course devoted solely to our microbiota, especially its positive impact. The course sold me on the importance of this field and its potential to change the way we look at some of the most important diseases that afflict us today.
Also, I am looking forward to your coverage of bacteriophages and phage related therapy, especially the future of its use in medicine.