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The Limitations of the Luria-Bertani Medium

Hiroshi Nakaido, PBD Faculty Scientist, Structural Biology Department, and Professor of Biochemistry and Molecular Biology, UC Berkeley, has authored a guest post on the Small Things Considered Blog regarding the limitations of LB medium.

"LB broth contains, per ml, 10 mg tryptone (... Read More

TWiM 73 Letters

Mark writes:


Hello Team TWiM,


I’ve followed with interest your coverage of Michael’s research into use of copper to fight hospital infection. Of all the interesting papers covered in 2013, I think the one most actionable is episode 55, The Copper Room. His res... Read More

The Exxon Valdez and Bioremediation

As we hear more and more news about the environmental disaster currently underway in the Gulf, there has been much talk about how microbes can be utilized to biodegrade the oil. In this 7 minute video posted on YouTube we see how scientists successfully implemented a bioremediation plan during t... Read More

TWiV 357: Mistletoe and the Tree of Life

Hosts: Vincent RacanielloDickson DespommierAlan Dove Read More

SketchyMicro teaches Klebsiella, Serratia and Enterobacter in a really memorable way!

SketchyMicro is a unique and effective way to learn high-yield medical microbiology for the USMLE Step 1.

They take all of the microorganisms, infectious diseases, and random facts that you need to memorize for USMLE Step 1, and weave them into easy-to-remember sketches.

They narrate as th... Read More

A short-short science fiction story about microbes...

From my microbiology-associated blog, where I discuss teaching and research in microbiology at a small liberal arts institution. Read More

A new rhabdovirus from a patient with hemorrhagic fever

Hemorrhagic fevers are among the most graphic viral diseases, inspiring movies, novels, and a general fear of infection. They are characterized by an abrupt onset and a striking clinical course involving bleeding from the nose and mouth, vomiting with blood, and bloody diarrhea. The most famous ... Read More

TWiM #119: Power of one

Hosts: Vincent RacanielloMichael Schmidt, and Read More

BacterioFiles Micro Edition 155 - Cells Save Superannuated Sequences

This episode: Bacteria are able to incorporate DNA from the environment into their genomes, even if it's thousands of years old!


(9.6 MB, 10.5 minutes)


Show notes: 
Read More

Everyday Roles

Some people think it would be great if scientists could wipe out all the microbial bugs! Should we do it, and why or why not?


We get a lot of requests to track down the nasty bugs that are making people sick. It's true that some microbes cause health problems such as strep throat, chi... Read More

TWiP 59 letters

Allan writes:


You have the best podcast on the web, hands down. I use your TWiP episodes in both my undergraduate and graduate classes.
Thanks.


I've recently seen a family in Hawaii with recurrent pathogenic Blastocystis hominis and Dientamoeba fragilis a... Read More

TWiV 305: Rhymes with shinola

Vincent, Alan, and Kathy continue their coverage of the Ebola virus outbreak in West Africa, with a discussion of case fatality ratio, reproductive index, a conspiracy theory, and spread of the virus to the United States.


Hosts:  Read More

The Basics: How Alkaline Lysis Works

Alkaline lysis was first described by Birnboim and Doly in 1979 (Nucleic Acids Res. 7, 1513-1523) and has, with a few modifications, been the preferred method for plasmid DNA extraction from bacteria ever since. The easiest way to describe how alkaline lysis works is to go through the procedure ... Read More

TWiV 358: Virology and proteomics with Ileana Cristea

Host: Vincent Racaniello


Guest: Ileana Cristea


Vincent meets up with Ileana at Princeton University to talk abou... Read More

TWiV 236: Flu gets the VIP treatment

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Hosts: Vincent Racaniello, Alan Dove, and Read More

BacterioFiles 214 - Cable-Chlorophyte Coop Conducts Current

This episode: Cable bacteria and algae set up electric grid in sediments!


(6 MB, 6.5 minutes)


Show notes: 
Journal Paper


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Microbial Genomics and Infectious Diseases

Would you like to read a concise and well-written review about how genomics has influenced our understanding of infectious diseases? Click "source" above for a satisfying account by David Relman, one of the leading contributors to this field.

After summarizing the dizzying accelerating pace a... Read More

Video: Cloning HeLa cells with Professor Philip I. Marcus

Video of my conversation from TWiV 197 with Professor Philip I. Marcus on his development of the single cell cloning technique in the 1950s, using HeLa cells. Read More

BacterioFiles Micro Edition 90 - Prokaryote Parasites Deal Double Dose Death

This episode: Discovery of a bacterium infected by two distinct organisms at the same time!



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Can You Protect Your Tummy From Traveler's Diarrhea?

It goes by many names: Delhi belly. Montezuma's revenge. The Aztec two-step. But doctors use one not-so-glamorous term: traveler's diarrhea. Read More

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