Alkaline lysis was first described by Birnboim and Doly in 1979 (Nucleic Acids Res. 7, 1513-1523) and has, with a few modifications, been the preferred method for plasmid DNA extraction from bacteria ever since. The easiest way to describe how alkaline lysis works is to go through the procedure ... Read More
Dear Vincent and Dick Just a quick message to say thank you for this informative and fun podcast series, I have enjoyed all three episodes and hope to listen to many more. Jusy the right thing for a former leishmaniac, now teache
Dickson reads his story about sparganosis caused by the diphyllobothroid tapeworm Spirometra mansonoides.
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Phenol extraction is a commonly used method for removing proteins from a DNA sample, e.g. to remove proteins from cell lysate during genomic DNA preparation. It’s commonly used, but not commonly understood.
This article describes the basic protocol and explains solvents in detail before an ex... Read More
One of the very first things you need to do when getting set up for quantitative PCR (qPCR) is to determine the efficiency of the assay because knowing the assay efficiency is critical to accurate data interpretation. And you have to do this every time you design and purchase a new primer pair.
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In a previous article, we discussed the basic characteristics of biofilm samples and factors that influence sample prep and handling. Today we want to share with you some very important tips for isolation of DNA or RNA from biofilm samples. After working with numerous different biofilms and bio... Read More
All mammalian genomes contain genes encoding Apobec proteins. Several members of this protein family (the name stands for apolipoprotein B mRNA editing complex) are induced by interferon and are intrinsic antiretroviral proteins. Apobec proteins inhibit the replication of XMRV, a new human retro... Read More
Theoretical physicist and futurist Michio Kaku looks at the revolution in genetics and biotechnology, which promises unprecedented health and longevity but also raises fears of a future where we can genetically engineer people. The documwentary asks will we, as transhumanists expect, evolve into... Read More
Jay Keasling, CEO of the Joint BioEnergy Institute, delivers the opening keynote on March 24, 2010 at the 5th Annual DOE JGI User Meeting. Read More
On 9 April 2010, the European Commission published the results of a Eurobarometer on antimicrobial resistance (AMR) which demonstrate the need for further progress on the issue in the European Union (EU) . The report highlights public attitudes towards the use of antibiotics which are of conc... Read More
Safe, cheap and effective adjunct therapies preventing the development of, or reducing the mortality from, severe malaria could have considerable and rapid public health impact. Oral activated charcoal (oAC) is a safe and well tolerated treatment for acute poisoning, more recently shown to have ... Read More
A new data resource for measuring the accuracy of metagenomic binning methods, created by in vitro-simulation of a metagenomic community, can be used to complement previous in silico benchmark studies. In constructing a synthetic community and sequencing its metagenome, researchers from the Univ... Read More
Hi guys really like the show even though some of it (not much) goes over my head. I also listen to twip and hope there will be a matching number of episodes to rival twiv. My name is Michael and I was talking to my dad the other day and he mentioned that he t... Read More
On episode #78 of the podcast This Week in Virology, Vincent, Alan, Dickson, and Rich talk about treating arthritis with a tanapox virus protein, Darwinian evolution of prions in cell culture, and t... Read More
Elio Schaechter of Small Things Considered defines the term "ontology" and why its destined to become part of every biologist’s vocabulary. Read More
A video from the DOE JGI '09 User Meeting on March 27, 2009 featuring Craig Venter's keynote talk "Reading and Writing the Genetic Code."
GEMINA is a web-based system to identify infectious pathogens and their representative genomic sequences through selection of associated epidemiology metadata. Gemina supports the development of DNA signature-based assays for the detection of pathogens or sets of pathogen through the Insignia Si... Read More
A new paper publish in PLoS One concludes that programs that optimize adherence to highly-active antiretroviral therapy (HAART) through direct observation in pregnancy have the potential to diminish mother-to-child HIV transmission in a highly cost-effective manner. Read More
It has always been assumed that plant viruses cannot infect animals, and vice versa, but plant viruses are known to be abundant in human faeces.
Now Didier Raoult at the University of the Mediterranean in Marseille, France, and his team think a pepper virus is making people sick, too.
They... Read More