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TWiP 8: Frog legs and parasite tales



Dickson reads his story about sparganosis caused by the diphyllobothroid tapeworm Spirometra mansonoides.


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MTS48 - Keith Klugman - Pneumonia: The Hidden Giant



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The Basics: How Phenol Extraction Works

Phenol extraction is a commonly used method for removing proteins from a DNA sample, e.g. to remove proteins from cell lysate during genomic DNA preparation. It’s commonly used, but not commonly understood.

This article describes the basic protocol and explains solvents in detail before an ex... Read More

Important Considerations for Determining qPCR Efficiency

One of the very first things you need to do when getting set up for quantitative PCR (qPCR) is to determine the efficiency of the assay because knowing the assay efficiency is critical to accurate data interpretation. And you have to do this every time you design and purchase a new primer pair.
... Read More

DO’s and DON’Ts for Isolation of DNA and RNA from Biofilm

In a previous article, we discussed the basic characteristics of biofilm samples and factors that influence sample prep and handling. Today we want to share with you some very important tips for isolation of DNA or RNA from biofilm samples. After working with numerous different biofilms and bio... Read More

Inhibition of XMRV by a weapon of mass deamination

All mammalian genomes contain genes encoding Apobec proteins. Several members of this protein family (the name stands for apolipoprotein B mRNA editing complex) are induced by interferon and are intrinsic antiretroviral proteins. Apobec proteins inhibit the replication of XMRV, a new human retro... Read More

Biotech Revolution (BBC Four)

Theoretical physicist and futurist Michio Kaku looks at the revolution in genetics and biotechnology, which promises unprecedented health and longevity but also raises fears of a future where we can genetically engineer people. The documwentary asks will we, as transhumanists expect, evolve into... Read More

Jay Keasling -Engineering Microbes to Produce Fuels

Jay Keasling, CEO of the Joint BioEnergy Institute, delivers the opening keynote on March 24, 2010 at the 5th Annual DOE JGI User Meeting. Read More

Eurobarometer on antimicrobial resistance highlights areas for action

On 9 April 2010, the European Commission published the results of a Eurobarometer on antimicrobial resistance (AMR) which demonstrate the need for further progress on the issue in the European Union (EU) [1]. The report highlights public attitudes towards the use of antibiotics which are of conc... Read More

Oral Activated Charcoal Prevents Experimental Cerebral Malaria in Mice and in a Randomized Controlled Clinical Trial in Man Did Not Interfere with the Pharmacokinetics of Parenteral Artesunate

Safe, cheap and effective adjunct therapies preventing the development of, or reducing the mortality from, severe malaria could have considerable and rapid public health impact. Oral activated charcoal (oAC) is a safe and well tolerated treatment for acute poisoning, more recently shown to have ... Read More

Metagenomic Sequencing of an In Vitro-Simulated Microbial Community

A new data resource for measuring the accuracy of metagenomic binning methods, created by in vitro-simulation of a metagenomic community, can be used to complement previous in silico benchmark studies. In constructing a synthetic community and sequencing its metagenome, researchers from the Univ... Read More

TWiV 78 letters

Michael writes:


Hi guys really like the show even though some of it (not much) goes over my head. I also listen to twip and hope there will be a matching number of episodes to rival twiv.  My name is Michael and I was talking to my dad the other day and he mentioned that he t... Read More

TWiV 78: Darwin gets weird



On episode #78 of the podcast This Week in Virology, Vincent, Alan, Dickson, and Rich talk about treating arthritis with a tanapox virus protein, Darwinian evolution of prions in cell culture, and the connect... Read More

Of Terms in Biology: Gene Ontology

Elio Schaechter of Small Things Considered defines the term "ontology" and why its destined to become part of every biologist’s vocabulary. Read More

Digitizing Biology with J. Craig Venter

A video from the DOE JGI '09 User Meeting on March 27, 2009 featuring Craig Venter's keynote talk "Reading and Writing the Genetic Code."

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GEMINA - a web-based system of Genomic Metadata for Infectious Agents

GEMINA is a web-based system to identify infectious pathogens and their representative genomic sequences through selection of associated epidemiology metadata. Gemina supports the development of DNA signature-based assays for the detection of pathogens or sets of pathogen through the Insignia Si... Read More

HAART has potential to diminish mother-to-child HIV transmission in cost-effective manner

A new paper publish in PLoS One concludes that programs that optimize adherence to highly-active antiretroviral therapy (HAART) through direct observation in pregnancy have the potential to diminish mother-to-child HIV transmission in a highly cost-effective manner. Read More

Could a plant virus have found a way to infect humans?

It has always been assumed that plant viruses cannot infect animals, and vice versa, but plant viruses are known to be abundant in human faeces.

Now Didier Raoult at the University of the Mediterranean in Marseille, France, and his team think a pepper virus is making people sick, too.

They... Read More

Paleovirology—Modern Consequences of Ancient Viruses

Here's an interesting essay published in PLoS Biology by Michael Emerman and Harmit S. Malik on paleovirology, a topic recently discussed by Welkin Johnson, on the Small Things Considered blog.


<... Read More

Bacterial Cells Engineered to Blink in Synch

Fluorescence-tagged Escherichia coli cells can be made to "blink" in unison by means of a constructed network of genes and proteins that coordinates oscillations within the growing cell population, according to Jeff Hasty and colleagues from the University of California, San Diego (UCSD) in La J... Read More

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