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Click-Chemistry Labeling of Oligonucleotides and DNA - Lumiprobe - Protocol


Click chemistry is a versatile reaction that can be used for the synthesis of a variety of conjugates. Virtually any biomolecules can be involved, and labeling with small molecules, such as fluorescent dyes, biotin, and other groups can be readily achieved.

Click chemistry reaction takes pl... Read More

TWiP 81 letters


Allan writes:


Dear Vincent & Dickson… and Daniel,


I always enjoy listening to TWIP here in Kona, Hawaii.


Our weather today is 79ºF and clear but we have just experienced a record 25-year overnight low of 54ºF,
(FREEZING, as few of us hav... Read More

My First Radio Interview About Teaching, Research, and Microbial Supremacy...

The great folks at "The People Behind the Science" interviewed me over Skype in late July, and the interview has just been released as a podcast. I discuss my path through science, my thoughts on teaching, research at undergraduate institution, and how to motivate and inspire students to strive... Read More

Where Biology and Art Work Together in the Classroom

In this blog entry, I describe how working with the artist Katie McKissick ("Beatrice the Biologist") helped improve my freshman biology course. The intersection of biology and art benefits both! Read More

Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate

Wine grapes present a unique biogeography model, wherein microbial biodiversity patterns across viticultural zones not only answer questions of dispersal and community maintenance, they are also an inherent component of the quality, consumer acceptance, and economic appreciation of a culturally ... Read More

ASM GM 2014 - The Potential Role of Gut Microbes in Autism

Most gut bacteria are beneficial, aiding food digestion, producing vitamins, and protecting against harmful bacteria. If left unchecked, however, harmful bacteria can excrete... Read More

Should you consider attending ASM 2015 this spring?

The general program for the 2015 American Society of Microbiology meeting in New Orleans is out, and registration is now open. The sessions look incredible, and I've listed them below as I imagine they might resonate with many of the readers of this blog. The bolded sessions look particularly in... Read More

Virology question of the week

On the science show This Week in Virology we receive many questions and comments, which are read every week. I also get many questions here on virology blog, which I tend to answer by email. However I think that everyone could benefit from these questions, so I’ve decided to post one here each w... Read More

Viroids, infectious agents that encode no proteins

Genomes of non-defective viruses range in size from 2,400,000 bp of dsDNA (Pandoravirus salinus) to 1,759 bp of ssDNA (porcine circovirus). Are even smaller viral genomes possible? The subviral agents called viroids provide an answer to this question.

Viroids, the smallest known pathogens, ar... Read More

C. difficile Vaccine Proves Safe, 100 Percent Effective In Animal Models

An experimental vaccine protected 100 percent of animal models against the highly infectious and virulent bacterium, Clostridium difficile, which causes an intestinal disease that kills approximately 30,000 Americans annually. The research is published ahead of print in Infection and Immunity.
... Read More

The Black Sheep of Microbe Family

Pleasing view-Microvista. Read More

ASM GM 2014 - Bacteria in Urine Could Cause Overactive Bladder

Contrary to popular belief, urine is not sterile and the bacteria in it may be associated with overactive bladder (OAB) in some women. Presenters will discuss their research ... Read More

Viral genomes in 700 year old caribou scat

Recovering viral genomes from ancient specimens can provide information about viral evolution, but not many old nucleic acids have been identified. A study of 700 year old caribou feces reveals that viruses can be protected for long periods of time – under the right conditions. Read More

Satellites - the viral kind

Satellites are subviral agents that differ from viroids because they depend on the presence of a helper virus for their propagation. Satellite viruses are particles that contain nucleic acid genomes encoding a structural protein that encapsidates the satellite genome. Satellite RNAs do not encod... Read More

Supercomputer Modelling of a Complete Human Viral Pathogen: Poliovirus (Polio virus)

Follow the reconstruction and simulation of poliovirus using the BlueGene/Q supercomputer at the Victorian Life Sciences Computation Initiative. The poliovirus model is being used as a basis for understanding antiviral drugs, virus infection and helps us to learn how to model related viruses suc... Read More

apple of my eye

I love to share my work,the simplicity in Happiness.I am sharing it here because i know my fellow microbiologist also admire this visual treats in their labs. http://adeebblog.blogspot.in/ Read More

How influenza virus infection might lead to gastrointestinal symptoms

Human influenza viruses replicate almost exclusively in the respiratory tract, yet infected individuals may also have gastrointestinal symptoms such as vomiting and diarrhea. In mice, intestinal injury occurs in the absence of viral replication, and is a consequence of viral depletion of the gut... Read More

TWiV 321: aTRIP and a pause

Paul Duprex joins the TWiV team to discuss the current moratorium on viral research to alter transmission, range and resistance, infectivity and immunity, and pathogenesis.


Hosts: Vincent Racaniel... Read More

ASM GM 2014 - The Effect of Pancreatic Cancer on the Oral Microbiome

In the United States, approximately 40,000 people die every year due to pancreatic adenocarcinoma, making it the fourth leading cause of cancer related death. Patients diagno... Read More

Zaire ebolavirus in West Africa

Dr. Tom Solomon is Director of the Institute for Infection and Global Health at the University of Liverpool. In this video he speaks with Vincent Racaniello about the 2014 outbreak of Zaire ebolavirus in West Africa. Dr. Solomon discusses why the epidemic has spread, how it might be curtailed, t... Read More
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