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Illustrating Some Simple Microbiological Concepts: Transformation, Antibiotic Resistance, and GFP.

In this blog post, I describe a simple "colony transformation" experiment I have done with my freshman students, illustrating transformation, antibiotic resistance, and GFP. The visual aspects help drive home the points effectivel. Read More

Henrietta Lacks (HeLa) genome sequence published then withdrawn

Earlier this month the European Molecular Biology Laboratory (EMBL) published the DNA sequence of the genome of HeLa cells, the cell line that is widely used for research in virology, cell biology, and many other areas. This cell line was produced from a tumor taken from Henrietta Lacks in 1951.... Read More

1 in 25 patients gets infection in hospital

When antibiotics first started being used in the 1940's they were considered a "miracle drug". It seemed that bacterial infections would no longer be a problem for the world. However, recently, one gene is making it seem as though the end of antibiotics is at hand. This gene is New Delhi metallo... Read More

TWiV 57

dooleyperiod_7-8_ab Read More

Important Considerations for Determining qPCR Efficiency

One of the very first things you need to do when getting set up for quantitative PCR (qPCR) is to determine the efficiency of the assay because knowing the assay efficiency is critical to accurate data interpretation. And you have to do this every time you design and purchase a new primer pair.
... Read More

Precious Metals

Moselio Schaechter of the Small Things Considered blog reviews the results of a recent paper "Microbial metalloproteomes are largely uncharacterized" from the Department of Biochemistry and Molecular Biology at the University of Georgia, Athens, and ponders its implications.

Snippet:

"Now... Read More

How Small Can You Get

parvovirus_B19 Read More

Deans write to Obama about CIA vaccine scheme in Pakistan

Deans of public health schools in the United States have sent a letter to President Obama, in which they criticize the use of a vaccination campaign by the Central Intelligence Agency in Pakistan to hunt for Osama bin Laden. I wonder if he will reply. Read More

TWiM #6 transcript

Here is a transcript of TWiM episode #6, "Antibacterial therapy with bacteriophage: Fact or fiction?". Thanks to Steve Stokowski for transcription.


The transcript is also available as a pdf file - click here to download Read More

TWiV 284: By the pricking of my thumbs, something wicked this way comes

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Hosts: Vincent RacanielloDickson Despommier Read More

El podcast del microbio Nº 258. Buscando a la Muerte Negra. (Searching for the Black Death)



























El podcast del Microbio Nº258 deals with the sequencing of the Yersinia pestis strain that causes the Black Death. El podcast de... Read More

El podcast del microbio Nº250. Cómo hacer un virus letal en cinco pasos (How to make a lethal virus in five steps)



























"El podcast del microbio" Nº 250 summarize the article published in November by New Scientist about the controversial work on mu... Read More

TWiM #49 transcript

Here is a transcript of TWiM episode #49, "Grape-like clusters". Thanks to Frank Shinneman for transcription.


The transcript is also available as a pdf file - click... Read More

Virus-induced fever might change bacteria from commensal to pathogen

Neisseria meningitidis may cause septicemia (bacteria in the blood) and meningitis (infection of the membrane surrounding the brain), but the bacterium colonizes the nasopharynx in 10-20% of the human population without causing disease. Although understanding how the bacterium changes from a com... Read More

TWiV 60

Eric asked for “advice on how to bridge the gap between clinical medicine, public health, and virology research.” I asked Scott Hammer MD for his thoughts on this question. Here is Dr. Hammer’s response:


Hi Vincent:


Thanks for forwarding this comment. In response, I’d inform y... Read More

Influenza Information (H1N1)

A one-page primer on the H1N1 flu and what you can do to protect yourself and your family. Read More

WHO will switch to type 2 inactivated poliovirus vaccine

The World Health Organization’s campaign to eradicate poliomyelitis made impressive inroads in 2012: only 212 cases were reported, compared with 620 the previous year; moreover, India remained polio-free. The dark side of this story is that as wild polio is eliminated, vaccine-associated poliomy... Read More

El podcast del Microbio Nº149. Physical Virology (Virología Física)



























El Podcast del Microbio" Nº 149 is based in the Nature Physics article by W Roos y G.J.L Wuite. El Podcast del Microbio Nº ... Read More

Poliovirus on Time

Poliovirus has made the cover of Time magazine. The Time cover image for the 14 January 2013 issue is a model of poliovirus bound to a soluble form of its cellular receptor, CD155. I was part of the team that solved the structure of this complex in 2000, together with the laboratories of Jim Hog... Read More

TWiV 292: Medimmune goes viral

Vincent visits Medimmune and speaks with Wade, Matt, Nicole, and Ken about why they work in industry and their daily roles in a biotechnology company.


Hosts: Vincent Racaniello


Guests: Wa... Read More

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