Alkaline lysis was first described by Birnboim and Doly in 1979 (Nucleic Acids Res. 7, 1513-1523) and has, with a few modifications, been the preferred method for plasmid DNA extraction from bacteria ever since. The easiest way to describe how alkaline lysis works is to go through the procedure ... Read More
A collection of funny images about microbiology. Although the text is in Spanish language, it can be easily understood. Read More
I really enjoyed your podcast about reverse transcription. I was wondering what makes retroviruses like HIV impossible for our bodies fight off. Are there any retroviruses that can be eliminated from the body?
I was browsing on the "Chronic ... Read More
This episode: Microbial predators in soil cause microbial prey to build fortifications!
(8.8 MB, 9.5 minutes)
Dear Dr. Schaechter,
First off, I want to thank you for the effort you and your colleagues have invested in Small Things Considered and the podcast TWIM. Back when I had a longer commute to work, I listened more religiously than now, wh... Read More
Dear Vincent and his fellow TWIMsters,
This episode: Programming bacteria to sense and keep genomic records of environmental inputs!
(15.9 MB, 17.4 minutes)
Hi Vincent and Dickson,
I enjoy TWIP, and often recommend it to my students. I'm a parasitologist, primarily a Leishmaniac, but I have learnt a lot from TWIP. I find it both more educational and entertaining than Car Talk.
The disc... Read More
This episode: Gut bacteria in Mojave desert woodrats help them detoxify and eat toxic creosote bushes!
(10 MB, 10.8 minutes)
Thank you for all you do, guys.
Steve writes... Read More
After the eradication of smallpox in 1980, the World Health Organization called for destruction of known remaining stocks of the virus. The United States and Russia, which hold the known stocks of smallpox virus, have not destroyed their stocks. During TWiV #124, I was surprised to learn that th... Read More
Hemorrhagic fevers are among the most graphic viral diseases, inspiring movies, novels, and a general fear of infection. They are characterized by an abrupt onset and a striking clinical course involving bleeding from the nose and mouth, vomiting with blood, and bloody diarrhea. The most famous ... Read More
Interfering with communication among bacteria can prevent them from mounting a unified and perhaps deadly assault on their host organism, research by Howard Hughes Medical Institute (HHMI) investigators shows. The finding suggests a different kind of medicine that could be less likely than tradi... Read More
The TWiV team reviews the discovery of old vials of smallpox virus at NIH, anthrax and influenza mishaps at CDC, the baby who was not cured of HIV, Cambridge Working Group, and sacking of NSABB members.
Hosts: Read More
This episode: Non-pathogenic Clostridium difficile strains can protect hamsters against their disease-causing bacterial siblings!
(7 MB, 7.5 minutes)
When hamsters were colonized with toxin-free strains of C. difficile, they were better able to resist infection b... Read More