Is bacterial DNA contamination in your whole genome amplification kit a problem? For microbiologists it sure is. WGA is a technique where the complete genomic content of a sample is amplified non-specifically and at a single temperature (isothermically). The presence of any contaminating DNA in ... Read More
A profile of Robert Blanchette, professor of plant pathology at the University of Minnesota, and his interesting work on fungi and the degradation archaeological wood. Read More
Hosts: Vincent Racaniello, Read More
Multiplicity of infection (MOI) is a frequently used term in virology which refers to the number of virions that are added per cell during infection. If one million virions are added to one million cells, the MOI is one. If ten million virions are added, the MOI is ten. Add 100,000 virions, and ... Read More
Human Lyme disease is caused by a number of related Borrelia burgdorferi sensu lato species. We report here the complete genome sequence of Borrelia sp. isolate SV1 from Finland. Read More
Hosts: Vincent Racaniello, Read More
Some additional info that I gathered on viruses on the verge of elimination (may or may not be new to you):
It appears that the next virus on the list of FAO to eradicate is PPR virus ( Read More
Derek Smith, Professor of Infectious Disease Informatics, University of Cambridge, U.K., has developed a method for visualizing antigenic evolution by creating two-dimensional maps in a process called antigenic cartography. These maps are made with data that provide information on the antigen... Read More
This episode: A study of the bacteria-hunting Bdellovibrio life cycle!
New York Times article by David Tuller, a journalism professor at Berkeley, on chronic fatigue syndrome and the retrovirus XMRV. The main focus of the article are four papers published in the journal Retrovirology at the end of 2010 which pointed to contamination as a potential issue for those a... Read More
A strong argument that the novel human retrovirus XMRV is not a laboratory contaminant is the the finding that viral DNA is integrated in chromosomal DNA of prostate tumors. Why does this result constitute such strong proof of viral infection?
Establishment of an integrated copy of the viral ... Read More
Moselio Schaechter of the Small Things Considered blog reviews the results of a recent paper "Microbial metalloproteomes are largely uncharacterized" from the Department of Biochemistry and Molecular Biology at the University of Georgia, Athens, and ponders its implications.
"Now... Read More
This episode: Bacteria help ants keep parasites out of their fungal gardens!
Vincent, Alan, and Rich revisit ten compelling virology stories of 2010.
Hosts: Read More
I am at present suffering from [what] I have been told will be a virus.
Symptoms:- a dry continuous hacking cough. Unable to get much sleep. No cough medicine seems to soothe it. An uncontrolled loss of urine when coughing. Otherwise no flu symptoms.... Read More
The protein components of the plaques and tangles seen in the Alzheimer's disease brain offer a clue to the origins of the disease: It turns out that they are heavily enriched in human proteins used by herpes simplex during its life cycle: Many immune system related proteins are also found in th... Read More
On my recent trip to record TWiV #111 at Florida Gulf Coast University, I visited Rich Condit in Gainesville. There he told me a story about how the bacteriophage T7 polymerase/promoter system was developed. It’s an interesting tale that demonstrates how important scientific advances often have ... Read More
Hi Vince, Dick, Alan and Rich,
My name is Trevor Stewart, a PhD student at the University of Toronto (in my final year!!) in Physics. I've been listening to TWIV (and TWIP) since last Xmas when my twin brother (who is a graduate student studying mass-s... Read More