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Foldscope: Origami-Based Paper Microscope

PLOS ONE authors Cybulski, Clements and Prakash describe an ultra-low-cost origami-based approach for large-scale manufacturing of microscopes, specifically demonstrating brightfield, darkfield, and fluorescence microscopes. Merging principles of optical design with origami enables high-volume f... Read More

MMP #1: Polio Virus Vesicles with Nihal Altan-Bonnet

Host: Jeff Fox Read More

Sushi protects mosquitoes from lethal virus infections

As far as I know, mosquitoes do not eat sushi. But mosquito cells have proteins with sushi repeat domains, and these proteins protect the brain from lethal virus infections. Read More

Overexpression of Enterococcus faecalis elr operon protects from phagocytosis

This study investigates how the overexpression of a specific ORF affects the virulence of E. faecalis. Read More

Implications of finding poliovirus in sewers of Brazil and Israel

Wild poliovirus has been detected in the sewers of Brazil and Israel. Fortunately, no cases of poliomyelitis have been reported in either country. Why is poliovirus present in these countries and what are the implications for the eradication effort?

Wild type poliovirus (e.g. not vaccine-deri... Read More

Viroids, infectious agents that encode no proteins

Genomes of non-defective viruses range in size from 2,400,000 bp of dsDNA (Pandoravirus salinus) to 1,759 bp of ssDNA (porcine circovirus). Are even smaller viral genomes possible? The subviral agents called viroids provide an answer to this question.

Viroids, the smallest known pathogens, ar... Read More

Surprise in Lab

Unknown fungi isolated from soil sample.I would like to have inputs from the Mycologist in the forum about this fungal colony,its identification,name,species and genus? Read More

HeLa RNA is everywhere

The first immortal human cell line ever produced, HeLa, originated from a cervical adenocarcinoma taken from Henrietta Lacks. The cell line grew so well that it was used in many laboratories and soon was found to contaminate other cell lines. Now HeLa RNA has made its way into human sequence dat... Read More

Teaching Freshmen about Transformation, Drug Resistance, and Gene Regulation!

In this blog post, I describe how I use the commonly available pGLO plasmid to teach freshman students basic concepts about transformation, receptor recognition, drug resistance mechanisms, and gene regulation. Oh, and fluorescence via GFP! Read More

Happy Birthday to a Microbial Hero™---Moselio Schaechter!

Until we have "action figures" of scientists, I do what I can to recognize scientists that have had a large and lasting influence on my academic career. In this blog post, I praise Dr. Moselio Schaechter for his clear and insightful approaches to microbiology. His enthusiasm, breadth of interes... Read More

A Bornavirus / host interactome

A referenced interactome of host genes and proteins affected by the Bornavirus. KEGG pathways of the host arm of the interactome are also linked from the interactome page. If you have any data to add please use the email link on the page and I will update the various pathways. Read More

Breathing New Life into DNA Microarrays

DNA microarrays are widely used in biological research and were once thought to be a very promising gene discovery tool. However, since the advent of next generation DNA sequencing, the popularity of DNA microarrays has steadily declined. Compared to the sequencing, microarray behavior is unce... Read More

Attenuated influenza vaccine enhances bacterial colonization of mice

Infection with influenza virus is known to increase susceptibility to bacterial infections of the respiratory tract. In a mouse model of influenza, increased bacterial colonization was also observed after administration of an infectious, attenuated influenza virus vaccine. Primary influenza viru... Read More

Let It (Microbially) Snow...

I made this video in October of 2015, with my Biology 350 Microbiology students at the University of Puget Sound. A drop of Pseudomonas syringae expressing ice nucleation protein hits supercooled pure water, and fun results. This is the way that Snomax works to commercially generate snow, inci... Read More

Happy Luxmas 2015!

In this blogpost, I celebrate the microbiological season with some microbial merriment: ice nucleation bacteria, drawing with reporter gene bacteria, and painting with bioluminescent microbes. Every day is Luxmas to me! Read More

Click-Chemistry Labeling of Oligonucleotides and DNA - Lumiprobe - Protocol


Click chemistry is a versatile reaction that can be used for the synthesis of a variety of conjugates. Virtually any biomolecules can be involved, and labeling with small molecules, such as fluorescent dyes, biotin, and other groups can be readily achieved.

Click chemistry reaction takes pl... Read More

Technological advances reveal finer details about chronic infection

How do chronic infections change over time? This is the broad question addressed in recent research published in the Journal of Virology. In their study, a team of scientists headed by Fabio Luciani investigated a hepatitis B virus infection over a course of 15 years.

Hepatitis B virus (HBV) ... Read More

Freshman Biology Creative Projects

I am a big believer that different pedagogical approaches can "reach" different students. In most of my classes, I give students an optional assignment: come up with a creative project that explores some aspect of class. This takes several steps. First, I make the students come up with an ide... Read More

Animals (and Plants) in a Microbial World

In this blog post (and fourth "Mu-Tube" video), I explore the idea that all animals and plants have evolved as part of a microbial world, and thus microbes are a part of us. I do this by having my undergraduate students explore two recently publications by Dr. Margaret McFall-Ngai,and recording... Read More

Study reveals intestinal bacteria succession during recovery from cholera in Bangladesh

A new study delineates a sequential pattern of changes in the intestinal microbial population of patients recovering from cholera in Bangladesh, findings that may point to ways of speeding recovery from the dangerous diarrheal disease. The report also finds what appear to be consistent differenc... Read More
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