DNA microarrays are widely used in biological research and were once thought to be a very promising gene discovery tool. However, since the advent of next generation DNA sequencing, the popularity of DNA microarrays has steadily declined. Compared to the sequencing, microarray behavior is unce... Read More
Reticuloendotheliosis viruses (REVs) are retroviruses that cause a rare disease of gamebirds and waterfowl that includes anemia, immunosuppression, neoplasia, runting, and abnormal feathering. Since the first isolation of REV from a turkey in 1957, REVs were believed to be strictly avian viruses... Read More
Emergence of antibiotic resistance and extended spectrum β- lactamase (ESBL) among uropathogens in the pediatric unit of hospitals created serious health care concern. This study deals with antimicrobial susceptibility and ESBL analysis of uropathogenic Escherichia coli isolate... Read More
In this blog entry, I discuss the perennial problem for educators: helping students find study strategies that help them reach their educational goals. I have found that student-generated study material is most helpful...and is sometimes quite artistic! Read More
Infection with influenza virus is known to increase susceptibility to bacterial infections of the respiratory tract. In a mouse model of influenza, increased bacterial colonization was also observed after administration of an infectious, attenuated influenza virus vaccine. Primary influenza viru... Read More
A new virus called Pithovirus sibericum has been isolated from 30,000 year old Siberian permafrost. It is the oldest DNA virus of eukaryotes ever isolated, showing that viruses can retain infectivity in nature for very long periods of time. Read More
In this blog post, I discuss how students begin to find their "path" to a career that they will love in science. I aIso write about the late, great Richard Feynman. Read More
In this blog post, I describe how I use the commonly available pGLO plasmid to teach freshman students basic concepts about transformation, receptor recognition, drug resistance mechanisms, and gene regulation. Oh, and fluorescence via GFP! Read More
A recent study by scientists at the Ludwig Center for Cancer Immunotherapy offers a new, multifaceted therapy for destroying tumors. A team of researchers led by Dmitriy Zamarin combined checkpoint blockade, a technique aimed at enhancing antitumor immune responses, with oncolytic viral therapy,... Read More
Protocols and free how to advice from Lumiprobe on how to use Fluorescent dyes, Click Chemistry reagents, Life Science
-Click-chemistry labeling of oligonucleotides and DNA.
-NHS ester labeling of amino biomolecules.
-SYBR Green I staining of DNA in gels.
-qPCR with SYBR Gree... Read More
In this post from my blog, I discuss the recent interest in snarky-funny academic hashtags on Twitter, such as #overlyhonestsyllabi. Just like students, educators need to blow off some steam (especially as classes approach!). But it is also important to remember our educational goals, and matc... Read More
Experiments with the most dangerous human viruses, such as Ebola virus and Lassa virus, are carried out in biosafety level 4 (BSL-4) laboratories. Since visiting the Northeast Infectious Diseases Laboratory BSL-4 and releasing the documentary video Threading the NEIDL, I was given the opportunit... Read More
In this blog post, I describe some "rules for academics" as well as my own "rules for research." I hope that readers find them useful and perhaps a bit humorous. Read More
You don’t want to miss asm2014. We’ve compiled a list of five things not to miss at the 114th American Society for Microbiology General Session.
Click the "source" link or copy/paste this URL into your browser: http://blog.puritanmedproducts.com/bid/383376/asm2014-Five-Things-Not-to-Miss-at-... Read More
The journal "RNA Biology" just published a memorial issue dedicated to the late, great Carl Woese. The entire issue is open access. I have a small contribution, but the entire issue is filled with great science, wonderful memories, and a fine celebration of a scientist who changed the way we l... Read More
It is a well recognized uncommon cause of skin and nail infection of the hands and feet . Unlike Dermatophytosis these mould infections are not contagious. There are few reports of invasive infection caused by N. dimidiatum; most infections occurred in immunocompromised hosts. Arthrospores ar... Read More
Segmented genomes abound in the RNA virus world. They are found in virus particles from different families, and can be double stranded (Reoviridae) or single stranded of (+) (Closteroviridae) or (-) (Orthomyxoviridae) polarity. Our recent discussion of the advantages of a segmented viral genome,... Read More
On this Mothers' Day, I reflect on the role my late mother had on my own decision to become a scientist. We all owe are mothers a great deal, and not just for the mitochondria! So hurray for mothers everywhere! Read More
Middle Eastern respiratory syndrome coronavirus (MERS-CoV), first identified in the fall of 2012 in a Saudi Arabian patient, has since infected over 160 individuals, causing 71 deaths. Identifying the source of infection is important for efforts to prevent further infections. Recently two studie... Read More