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How to use Fluorescent dyes or Click Chemistry in research

Protocols and free how to advice from Lumiprobe on how to use Fluorescent dyes, Click Chemistry reagents, Life Science
-Click-chemistry labeling of oligonucleotides and DNA.
-NHS ester labeling of amino biomolecules.
-SYBR Green I staining of DNA in gels.
-qPCR with SYBR Gree... Read More

Report on Viruses Looks Beyond Disease

Recently, the American Academy of Microbiology released a new report, Viruses Throughout Life & Time: Friends, Foes, Change Agents that delves into the origin of viruses, the overlooked biological and microbial ecological role of viruses, and how these live forms have contributed to evolution. T... Read More

ASM GM 2014 - Microbes and Cancer

Until recently cancers were seen as lifestyle and genetic diseases, brought on by exposure to carcinogens or a mutated gene. Recent studies are linking microbes to many diffe... Read More

Pet rats as a source of hantavirus in England and Wales

Researchers report the detection of a strain of Seoul hantavirus (SEOV) in pet rats in England and Wales. The discovery followed an investigation of a case of haemorrhagic fever with renal syndrome in Wales. Hantavirus RNA was detected via real-time reverse transcription-polymerase chain reactio... Read More

Foldscope: Origami-Based Paper Microscope

PLOS ONE authors Cybulski, Clements and Prakash describe an ultra-low-cost origami-based approach for large-scale manufacturing of microscopes, specifically demonstrating brightfield, darkfield, and fluorescence microscopes. Merging principles of optical design with origami enables high-volume f... Read More

Mothers' Day, Marcus Aurelius, and Microbiology.

On this Mothers' Day, I reflect on the role my late mother had on my own decision to become a scientist. We all owe are mothers a great deal, and not just for the mitochondria! So hurray for mothers everywhere! Read More

Where Biology and Art Work Together in the Classroom

In this blog entry, I describe how working with the artist Katie McKissick ("Beatrice the Biologist") helped improve my freshman biology course. The intersection of biology and art benefits both! Read More

El podcast del microbio Nº 378. Llave Falsa (False Key)



























El podcast del microbio Nº 378 summarize an article published in Nature communications by Ribeiro-Viana R et al. on the use of Virus-like glycodendrinanoparticles to block viral infection. El podcast del microbio Nº 3... Read More

Microbial biogeography of wine grapes is conditioned by cultivar, vintage, and climate

Wine grapes present a unique biogeography model, wherein microbial biodiversity patterns across viticultural zones not only answer questions of dispersal and community maintenance, they are also an inherent component of the quality, consumer acceptance, and economic appreciation of a culturally ... Read More

ASM GM 2014 - Windshield Wiper Fluid: A Source of Legionnaires?

A form of bacteria responsible for respiratory illness, including the deadly pneumonia known as Legionnaire's disease, may be able to grow in windshield washer fluid and was isolated from nearly 75% of school buses tested in one district in Arizona. The participant will discuss findings from ... Read More

Click-Chemistry Labeling of Oligonucleotides and DNA - Lumiprobe - Protocol


Click chemistry is a versatile reaction that can be used for the synthesis of a variety of conjugates. Virtually any biomolecules can be involved, and labeling with small molecules, such as fluorescent dyes, biotin, and other groups can be readily achieved.

Click chemistry reaction takes pl... Read More

Influenza A viruses in bats

It is well known that aquatic birds are a major reservoir of influenza A viruses, and that pandemic human influenza virus strains of the past century derive viral genes from this pool. The recent discovery of two new influenza A viruses in bats suggests that this species may constitute another r... Read More

Virology question of the week

On the science show This Week in Virology we receive many questions and comments, which are read every week. I also get many questions here on virology blog, which I tend to answer by email. However I think that everyone could benefit from these questions, so I’ve decided to post one here each w... Read More

Remembering a Microbial Hero™: The Late, Great Abigail Salyers.

Here I remember a person who a great deal of influence on my views about microbiology in the classroom, and the laboratory: the late Abigail Salyers. RIP, Abigail. Read More

Probiotics vs Antibiotics? You're asking the wrong question.

'The question of whether someone should be taking a probiotic or an antibiotic is one I hear fairly frequently. The answer, in short, is that this isn't a question of either / or! At this stage in time, with this level of research on probiotics, it is not right to suggest one stops taking their... Read More

For US$16,818.50 would you want a synthetic human virus?

In the media aftermath of the H5N1 transmission debate we've been hearing an awful lot about the possibilities of bringing synthetic biology to the field of virology. In fact, one of the best analyses of this situation is Carl Zimmer's piece in the New York Times. In it, Carl explores the capabi... Read More

ASM GM 2014 - Fossilized Feces Help Anthropologists Understand Pre-Columbian Cultures

By evaluating the bacteria and fungi found in fossilized feces, microbiologists are providing evidence to help support archeologists' hypotheses regarding cultures living in the Caribbean over 1,500 years ago. Researchers discuss how the analysis of 1,500-year-old coprolites from archeologica... Read More

ASM GM 2014 - ASM ¡en vivo!

ASM2014 tiene "sabor Latino". Por primera vez podrás participar de ASM ¡en vivo! Tendremos una sección solo en Español donde las anfitrionas, Greetchen y Catalina (Mundo de l... Read More

Changing influenza virus neuraminidase into a receptor binding protein

The hemagglutinin (HA) and neuraminidase (NA) glycoproteins of the influenza virus particle serve distinct functions during infection. The HA binds sialic acid-containing cellular receptors and mediates fusion of the viral and cell membranes, while the NA removes sialic acids from glycoproteins.... Read More

ASM GM 2014 - The Potential Role of Gut Microbes in Autism

Most gut bacteria are beneficial, aiding food digestion, producing vitamins, and protecting against harmful bacteria. If left unchecked, however, harmful bacteria can excrete... Read More

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