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Implications of finding poliovirus in sewers of Brazil and Israel

Wild poliovirus has been detected in the sewers of Brazil and Israel. Fortunately, no cases of poliomyelitis have been reported in either country. Why is poliovirus present in these countries and what are the implications for the eradication effort?

Wild type poliovirus (e.g. not vaccine-deri... Read More

Origin of segmented RNA virus genomes

Segmented genomes abound in the RNA virus world. They are found in virus particles from different families, and can be double stranded (Reoviridae) or single stranded of (+) (Closteroviridae) or (-) (Orthomyxoviridae) polarity. Our recent discussion of the advantages of a segmented viral genome,... Read More

Overexpression of Enterococcus faecalis elr operon protects from phagocytosis

This study investigates how the overexpression of a specific ORF affects the virulence of E. faecalis. Read More

Stability of MERS Under Different Environmental Conditions (research)

The stability of Middle East respiratory syndrome coronavirus (MERS-CoV) was determined at 20°C – 40% relative humidity (RH); 30°C – 30% RH and 30°C – 80% RH. MERS-CoV was more stable at low temperature/low humidity conditions and could still be recovered after 48 hours. During aerosolisation of... Read More

Attenuated influenza vaccine enhances bacterial colonization of mice

Infection with influenza virus is known to increase susceptibility to bacterial infections of the respiratory tract. In a mouse model of influenza, increased bacterial colonization was also observed after administration of an infectious, attenuated influenza virus vaccine. Primary influenza viru... Read More

The science-themed art of Deb Sklut

During my visit to Berkeley, CA to record TWiV #228, I met Deb Sklut, an artist who is inspired by the power of science. I recorded a brief conversation with Deb which you can view below. Her work can be found at SqueakySqueegeeArt.etsy.com. Read More

Foldscope: Origami-Based Paper Microscope

PLOS ONE authors Cybulski, Clements and Prakash describe an ultra-low-cost origami-based approach for large-scale manufacturing of microscopes, specifically demonstrating brightfield, darkfield, and fluorescence microscopes. Merging principles of optical design with origami enables high-volume f... Read More

Study reveals intestinal bacteria succession during recovery from cholera in Bangladesh

A new study delineates a sequential pattern of changes in the intestinal microbial population of patients recovering from cholera in Bangladesh, findings that may point to ways of speeding recovery from the dangerous diarrheal disease. The report also finds what appear to be consistent differenc... Read More

Isolation of a Novel Antibiotic Resistance Plasmid DNA from Hospital Isolates of Pseudomonas aeruginosa

Emergence and dissemination of antibiotic resistance plasmids are major concern for hospital care system and
increases the cost and decreases effectiveness of available antibiotics used in treatment of hospitalized patients.
In this study two Pseudomonas aeruginosa, two Escherichia coli, and a... Read More

Avian influenza H7N7 virus outbreak: Lessons for H7N9

An outbreak of high-pathogenicity avian influenza H7N7 virus that took place on 255 poultry farms in the Netherlands during 2003 has been used to provide clues about the current avian influenza H7N9 viruses in China. During the Dutch outbreak 453 humans showed symptoms of illness and 89 were con... Read More

Teaching Freshmen about Transformation, Drug Resistance, and Gene Regulation!

In this blog post, I describe how I use the commonly available pGLO plasmid to teach freshman students basic concepts about transformation, receptor recognition, drug resistance mechanisms, and gene regulation. Oh, and fluorescence via GFP! Read More

Satellites - the viral kind

Satellites are subviral agents that differ from viroids because they depend on the presence of a helper virus for their propagation. Satellite viruses are particles that contain nucleic acid genomes encoding a structural protein that encapsidates the satellite genome. Satellite RNAs do not encod... Read More

Measles in the brain: Fusion gone awry

The entry of enveloped viruses into cells begins when the membrane that surrounds these virus particles fuse with a cell membrane. The process of virus-cell fusion must be tightly regulated, to make sure it happens in the right cells. The fusion activity of measles viruses isolated from the brai... Read More

Viral gifts

If you are looking for the perfect present for that infectious person in your life, there are many microbe-themed possibilities. Here are some suggestions, from t-shirts, to watercolors, glass sculptures, and paper to wrap it in. Read More

MERS-CoV genome found in dromedary camels

Middle Eastern respiratory syndrome coronavirus (MERS-CoV), first identified in the fall of 2012 in a Saudi Arabian patient, has since infected over 160 individuals, causing 71 deaths. Identifying the source of infection is important for efforts to prevent further infections. Recently two studie... Read More

ASM GM 2014 - The Effect of Pancreatic Cancer on the Oral Microbiome

In the United States, approximately 40,000 people die every year due to pancreatic adenocarcinoma, making it the fourth leading cause of cancer related death. Patients diagno... Read More

HeLa RNA is everywhere

The first immortal human cell line ever produced, HeLa, originated from a cervical adenocarcinoma taken from Henrietta Lacks. The cell line grew so well that it was used in many laboratories and soon was found to contaminate other cell lines. Now HeLa RNA has made its way into human sequence dat... Read More

Viroids, infectious agents that encode no proteins

Genomes of non-defective viruses range in size from 2,400,000 bp of dsDNA (Pandoravirus salinus) to 1,759 bp of ssDNA (porcine circovirus). Are even smaller viral genomes possible? The subviral agents called viroids provide an answer to this question.

Viroids, the smallest known pathogens, ar... Read More

Freshman Biology Creative Projects

I am a big believer that different pedagogical approaches can "reach" different students. In most of my classes, I give students an optional assignment: come up with a creative project that explores some aspect of class. This takes several steps. First, I make the students come up with an ide... Read More

Sushi protects mosquitoes from lethal virus infections

As far as I know, mosquitoes do not eat sushi. But mosquito cells have proteins with sushi repeat domains, and these proteins protect the brain from lethal virus infections. Read More
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