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Cyanine dyes - Non-sulfonated and Sulfonated cyanines Lumiprobe explains

Sulfonated and non-sulfonated cyanines exhibit very similar fluorescent properties. Non-sulfonated cyanines must be dissolved in organic co-solvent (DMF or DMSO) prior to use, and added to a solution of target molecule in aqueous buffers. Recommended volume of co-solvent should be 10% for Cy3, C... Read More

Curious about the Human Microbiome?

The American Academy of Microbiology has released its newest report on the human microbiome. Based on the deliberations of some of the leading experts, the report answers common questions people have about this new field of science. While there is still much to be learned, this report presents t... Read More

TWiP 66 letters

 


Andre writes:


Dear Vincent,


To my great delight, I just discovered your podcasts twiv, twim and twip.


The first twip I heard, about Strongyloides stercoralis, although informative and interesting, seemed to have several inaccuracies. I w... Read More

India has been free of polio for three years

Three years ago today, on 13 January 2011, the last case of poliomyelitis was reported in India. This achievement represents a remarkable turnaround for a country where control of the disease had for years been extremely difficult. As recently as 2009 there were 741 confirmed cases of polio caus... Read More

TWiV 281: The Salk legacy with Peter L. Salk

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Host: Vincent Racaniello


Guest: Peter L. Salk


Vincent meets up with Peter L. Salk to talk about development ... Read More

TWiV 291: Ft. Collins abuzz with virologists

Vincent, Rich, and Kathy and their guests Clodagh and Ron recorded this episode at the 33rd annual meeting of the American Society for Virology at Colorado State University in Ft. Collins, Colorado.


Hosts: ... Read More

El Podcast del Microbio Nº 361. Resistencia a los antibióticos, un problema de narices. (Antibiotic resistance, just under your nose)



























El podcast del microbio Nº 361 summarize the article writen by Marks LR, Reddinger RM and Hakansson AP and published in mBio about the high levels of genetic recombination in Streptococcus pneumoniae ... Read More

A virology course for all

The spring semester has begun at Columbia University, which means that it is time to teach my virology course. The fourth annual installment of my virology course, Biology W3310, has begun. This course, which I taught for the first time in 2009, is intended for advanced undergraduates and conven... Read More

TWiV 265: This year in virology

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Hosts: Vincent Racaniello, Alan Dove Read More

TWiV special: MERS-coronavirus in dromedary camels

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Host: Vincent Racaniello


Guests: Read More

HIV gets the zinc finger

Because all animal viruses initiate infection by binding to a receptor on the cell surface, this step has long been considered a prime target for antiviral therapy. Unfortunately, drugs that block virus attachment to cells have never shown much promise. Another approach, which is to ablate the r... Read More

El podcast del microbio Nº 255: Hongos contra el picudo rojo (Fungi against the red palm weevil )



























El podcast del Microbio Nº255 explains the use of the fungus Beauveria bassiana as bioinsecticide for the control of the red pa... Read More

Henrietta Lacks (HeLa) genome sequence published then withdrawn

Earlier this month the European Molecular Biology Laboratory (EMBL) published the DNA sequence of the genome of HeLa cells, the cell line that is widely used for research in virology, cell biology, and many other areas. This cell line was produced from a tumor taken from Henrietta Lacks in 1951.... Read More

Illustrating Some Simple Microbiological Concepts: Transformation, Antibiotic Resistance, and GFP.

In this blog post, I describe a simple "colony transformation" experiment I have done with my freshman students, illustrating transformation, antibiotic resistance, and GFP. The visual aspects help drive home the points effectivel. Read More

1 in 25 patients gets infection in hospital

When antibiotics first started being used in the 1940's they were considered a "miracle drug". It seemed that bacterial infections would no longer be a problem for the world. However, recently, one gene is making it seem as though the end of antibiotics is at hand. This gene is New Delhi metallo... Read More

Deans write to Obama about CIA vaccine scheme in Pakistan

Deans of public health schools in the United States have sent a letter to President Obama, in which they criticize the use of a vaccination campaign by the Central Intelligence Agency in Pakistan to hunt for Osama bin Laden. I wonder if he will reply. Read More

TWiV 284: By the pricking of my thumbs, something wicked this way comes

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Hosts: Vincent RacanielloDickson Despommier Read More

TWiM #49 transcript

Here is a transcript of TWiM episode #49, "Grape-like clusters". Thanks to Frank Shinneman for transcription.


The transcript is also available as a pdf file - click... Read More

El podcast del microbio Nº250. Cómo hacer un virus letal en cinco pasos (How to make a lethal virus in five steps)



























"El podcast del microbio" Nº 250 summarize the article published in November by New Scientist about the controversial work on mu... Read More

El podcast del microbio Nº 258. Buscando a la Muerte Negra. (Searching for the Black Death)



























El podcast del Microbio Nº258 deals with the sequencing of the Yersinia pestis strain that causes the Black Death. El podcast de... Read More

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