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Breathing New Life into DNA Microarrays

DNA microarrays are widely used in biological research and were once thought to be a very promising gene discovery tool. However, since the advent of next generation DNA sequencing, the popularity of DNA microarrays has steadily declined. Compared to the sequencing, microarray behavior is unce... Read More

TWiP 82 letters


Allan writes:


Dear Vincent, Dickson and Daniel,


I like your idea of a TWIP coffee mug prize (or maybe a mug discount).


In this second case study you presented, the present symptoms are pretty vague, but his history is interesting. Also since with ... Read More

TWiV 323: A skid loader full of viromes

Hosts: Vincent RacanielloDickson DespommierAlan Dove, Read More

VIBRIO CHOLERAE O1 ISOLATEDFROM HUMAN STOOL

THIS IS VIBRIO CHOLERAE O1 OGAWA ISOLATED FROM HUMAN STOOL SWAB PLATED ON BILE SALT AGAR Read More

ASM GM 2014 - Windshield Wiper Fluid: A Source of Legionnaires?

A form of bacteria responsible for respiratory illness, including the deadly pneumonia known as Legionnaire's disease, may be able to grow in windshield washer fluid and was isolated from nearly 75% of school buses tested in one district in Arizona. The participant will discuss findings from ... Read More

ASM GM 2014 - Microbes and Cancer

Until recently cancers were seen as lifestyle and genetic diseases, brought on by exposure to carcinogens or a mutated gene. Recent studies are linking microbes to many diffe... Read More

BacterioFiles 203 - P. putida Promotes Plant Pollutant Purification

This episode: Bacteria living in plants could help plants clean up cancer-causing pollutants!


(6.9 MB, 7.5 minutes)


Show notes: 
News item<... Read More

Therapeutic teamwork: Coupling oncolytic viruses with immunotherapy to destroy tumor cells

A recent study by scientists at the Ludwig Center for Cancer Immunotherapy offers a new, multifaceted therapy for destroying tumors. A team of researchers led by Dmitriy Zamarin combined checkpoint blockade, a technique aimed at enhancing antitumor immune responses, with oncolytic viral therapy,... Read More

Pithovirus: Bigger than Pandoravirus with a smaller genome

A new virus called Pithovirus sibericum has been isolated from 30,000 year old Siberian permafrost. It is the oldest DNA virus of eukaryotes ever isolated, showing that viruses can retain infectivity in nature for very long periods of time. Read More

Origin of segmented RNA virus genomes

Segmented genomes abound in the RNA virus world. They are found in virus particles from different families, and can be double stranded (Reoviridae) or single stranded of (+) (Closteroviridae) or (-) (Orthomyxoviridae) polarity. Our recent discussion of the advantages of a segmented viral genome,... Read More

Merry Luxmas and Happy Microbial Holidays to All!

Here is my annual blogpost using #MicrobialSupremacy to wish all readers a very, very happy holiday season. I do this GFP and prodiosin, as well as luciferase! Enjoy a tiny bit of microbial art, relevant to the season? Read More

TWiM 97 Letters


Dennis writes:
hi Doc,


A paper just published in nature:


http://www.nature.com/nature/journal/vaop/ncurrent/pdf/nature14098.pdf


reports an effective antibiot... Read More

Implications of finding poliovirus in sewers of Brazil and Israel

Wild poliovirus has been detected in the sewers of Brazil and Israel. Fortunately, no cases of poliomyelitis have been reported in either country. Why is poliovirus present in these countries and what are the implications for the eradication effort?

Wild type poliovirus (e.g. not vaccine-deri... Read More

TWiP 73 letters

Robin writes:


Liquids


Liquids are capable of forming a gas-liquid interface: maintenance of the surface requires adequate pressure in the gas. Adequate in this instance means a gas pressure greater than the vaporisation pressure in the liquid. The gas pressure... Read More

Nobel Laureates and Ebola virus quarantine

After the governors of New York and New Jersey decided that health workers who have returned from the Ebola virus outbreak in West Africa should be subject to a 21-day quarantine, two Nobel laureates entered the fray. Bruce Beutler feels that the quarantine is the right thing to do, while Peter ... Read More

TWiV 324: Viruses in the miR may appear more numerous

Hosts: Vincent RacanielloDickson DespommierAlan Dove Read More

Attenuated influenza vaccine enhances bacterial colonization of mice

Infection with influenza virus is known to increase susceptibility to bacterial infections of the respiratory tract. In a mouse model of influenza, increased bacterial colonization was also observed after administration of an infectious, attenuated influenza virus vaccine. Primary influenza viru... Read More

Probiotics vs Antibiotics? You're asking the wrong question.

'The question of whether someone should be taking a probiotic or an antibiotic is one I hear fairly frequently. The answer, in short, is that this isn't a question of either / or! At this stage in time, with this level of research on probiotics, it is not right to suggest one stops taking their... Read More

Teaching Freshmen about Transformation, Drug Resistance, and Gene Regulation!

In this blog post, I describe how I use the commonly available pGLO plasmid to teach freshman students basic concepts about transformation, receptor recognition, drug resistance mechanisms, and gene regulation. Oh, and fluorescence via GFP! Read More

Foldscope: Origami-Based Paper Microscope

PLOS ONE authors Cybulski, Clements and Prakash describe an ultra-low-cost origami-based approach for large-scale manufacturing of microscopes, specifically demonstrating brightfield, darkfield, and fluorescence microscopes. Merging principles of optical design with origami enables high-volume f... Read More
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