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Click-Chemistry Labeling of Oligonucleotides and DNA - Lumiprobe - Protocol


Click chemistry is a versatile reaction that can be used for the synthesis of a variety of conjugates. Virtually any biomolecules can be involved, and labeling with small molecules, such as fluorescent dyes, biotin, and other groups can be readily achieved.

Click chemistry reaction takes pl... Read More

Influenza A viruses in bats

It is well known that aquatic birds are a major reservoir of influenza A viruses, and that pandemic human influenza virus strains of the past century derive viral genes from this pool. The recent discovery of two new influenza A viruses in bats suggests that this species may constitute another r... Read More

ASM GM 2014 - Windshield Wiper Fluid: A Source of Legionnaires?

A form of bacteria responsible for respiratory illness, including the deadly pneumonia known as Legionnaire's disease, may be able to grow in windshield washer fluid and was isolated from nearly 75% of school buses tested in one district in Arizona. The participant will discuss findings from ... Read More

Remembering a Microbial Hero™: The Late, Great Abigail Salyers.

Here I remember a person who a great deal of influence on my views about microbiology in the classroom, and the laboratory: the late Abigail Salyers. RIP, Abigail. Read More

Virology question of the week

On the science show This Week in Virology we receive many questions and comments, which are read every week. I also get many questions here on virology blog, which I tend to answer by email. However I think that everyone could benefit from these questions, so I’ve decided to post one here each w... Read More

Probiotics vs Antibiotics? You're asking the wrong question.

'The question of whether someone should be taking a probiotic or an antibiotic is one I hear fairly frequently. The answer, in short, is that this isn't a question of either / or! At this stage in time, with this level of research on probiotics, it is not right to suggest one stops taking their... Read More

Changing influenza virus neuraminidase into a receptor binding protein

The hemagglutinin (HA) and neuraminidase (NA) glycoproteins of the influenza virus particle serve distinct functions during infection. The HA binds sialic acid-containing cellular receptors and mediates fusion of the viral and cell membranes, while the NA removes sialic acids from glycoproteins.... Read More

Watch This Week in Virology Episode 300 Live from ASM HQ Aug 26 7 pm ET

This Week in Virology, the podcast about viruses, celebrates its 300th episode on Tuesday, August 26, 2014 with a live recording at the Washington, DC headquarters of the American Society for Microbiology. This special episode w... Read More

ASM GM 2014 - Fossilized Feces Help Anthropologists Understand Pre-Columbian Cultures

By evaluating the bacteria and fungi found in fossilized feces, microbiologists are providing evidence to help support archeologists' hypotheses regarding cultures living in the Caribbean over 1,500 years ago. Researchers discuss how the analysis of 1,500-year-old coprolites from archeologica... Read More

ASM GM 2014 - ASM ¡en vivo!

ASM2014 tiene "sabor Latino". Por primera vez podrás participar de ASM ¡en vivo! Tendremos una sección solo en Español donde las anfitrionas, Greetchen y Catalina (Mundo de l... Read More

World Polio Day

As a virologist who has worked on poliovirus since 1979, I would be remiss if I did not note that today, 24 October, is World Polio Day. World Polio Day was established by Rotary International over a decade ago to commemorate the birth of Jonas Salk, who led the first team to develop a vaccine a... Read More

ASM GM 2014 - The Potential Role of Gut Microbes in Autism

Most gut bacteria are beneficial, aiding food digestion, producing vitamins, and protecting against harmful bacteria. If left unchecked, however, harmful bacteria can excrete... Read More

C. difficile Vaccine Proves Safe, 100 Percent Effective In Animal Models

An experimental vaccine protected 100 percent of animal models against the highly infectious and virulent bacterium, Clostridium difficile, which causes an intestinal disease that kills approximately 30,000 Americans annually. The research is published ahead of print in Infection and Immunity.
... Read More

BacterioFiles 179 - Functionless Phages Feel Fatiguing

 This episode: Defective phages in bacterial genomes can still have burdensome effects! Why do the bacteria keep them around?


(10.4 MB, 11.3 minutes)


Show notes: 
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How a microscopic team alters the course of carbon in the Atlantic ocean - Finalist in Ocean 180 Video Challenge

The Amazon river is the largest river in the world. It drains the entire Amazon rainforest, sending leftover nutrients, detritus, and minerals from the South American jungle out into the tropical Atlantic ocean. This runoff forms a freshwater plume, hundreds of miles across, that profoundly affe... Read More

TWiV 298: MV-NIS de myelo

The TWiV gang answers follow-up questions about the Ebola virus outbreak in West Africa, then discuss treatment of  disseminated multiple myeloma with oncolytic measles virus.


Hosts: Vincent Racaniello... Read More

Innocence by Viral Tagging - Finalist in Ocean 180 Video Challenge

Viruses are the most abundant biological entities on the planet with approximately 1030 in the world’s oceans at any time. As such, they play a central role in global nutrient cycling. Despite their ecological importance, little is known about how viruses interact with their hosts due to the dif... Read More

ASM GM 2014 - Bacteria in Urine Could Cause Overactive Bladder

Contrary to popular belief, urine is not sterile and the bacteria in it may be associated with overactive bladder (OAB) in some women. Presenters will discuss their research ... Read More

Reduced glycopeptide and lipopeptide susceptibility in Staphylococcus aureus and the “seesaw effect”: Taking advantage of the back door left open?

Methicillin-resistant S. aureus (MRSA) constitutes approximately 50% of clinical S. aureus isolates and is most commonly the result of production of a mutated pencillin-binding protein, PBP2a, which is able to carry out essential cell wall synthesis functions while maintaining a low-affinity for... Read More

Molecular mechanisms of resistance

The abilities of bacterial organisms to utilize the various strategies to resist antimicrobial compounds are all genetically encoded.

Intrinsic resistance is that type of resistance which is naturally coded and expressed by all (or almost all) strains of that particular bacterial species. An... Read More

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