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Letters

TWiP 44 Letters

Todd writes:

Hello Dixon and Vincent!

Your research and real life experience is slowly getting summarized by researchers so that it can be understood by reporters (aka those who
have a disproportionately loud voice in society):

http://www.nytimes.com/2012/08/26/opinion/sunday/immune-disorders-and-autism.html?_r=1&src=me&ref=general

The article is attempting to tie lack of MPV (microbes, parasites, viruses) to inflammatory diseases, which might be a stretch. I don't know if there is a dearth or an abundance of research for either side of this proposed relationship. But it's promising on the surface and hopefully the political forces at work destroying science in our country won't do the same for this research.

An excerpt from the concluding paragraphs:

"Since time immemorial, a very specific community of organisms - microbes, parasites, some viruses - has aggregated to form the human superorganism. Mounds of evidence suggest that our immune system anticipates these inputs and that, when they go missing, the organism comes unhinged."

Have a fantastic week fellas!

Josh writes:

Hello Parasite hosts!

I have written twip before to express my appreciation for the show. I still appreciate it very much and it still helps me get through my days auditing.

I ran across this article on reddit.com/r/science, http://lifestyle.iafrica.com/wellness/813618.html , which makes this discovery sound like a guaranteed cure for all forms of Malaria with just one oral dose. Thinking that was a rather large claim I did some digging and found the original story, http://www.science.uct.ac.za/news/?id=8220&t=dn , this article is a little more reserved, though not much, with its language. I am interested in what both of you think of this? Is this the wonder drug they make it seem like?

Thanks,

Josh
Phoenix AZ
Auditor

Ricardo writes:

South African researcher find single dose cure for malaria: http://www.treehugger.com/health/south-african-researchers-find-single-dose-cure-for-malaria.html

Dirk writes:

Dear parasitology lovers,

Just came across your blog through recommendation of a colleague and heard your conversation on the worm in the eye.

The name seems to be the local meaning of "worm" as quoted on page 643 in the book "A HISTORY OF HUMANHELMINTHOLOGY by DAVID I. GROVE (link: http://www.scribd.com/doc/57580301/Book) from the publication #49: GUYOT. Ophthalmie produite par des vers dans les yeux à la côte d'Angole. Abstracted in J N Arrachart's Mémoires, Dissertations et Observations de Chirurgie, Paris, pp228-233, 1805. (Originally presented to the Academy of Surgery in Paris in 1778).

And if I understand correctly you claimed that Cobbold was a German. Although being a German myself Cobbold was British, a son of a Suffolk clergyman (Principles and Practice of Clinical Parasitology, Gillespie /Pearson, 2001, page 3.

Best regards,

Dirk

Lisa writes:

Dear Vincent and Dickson,

I am a veterinarian with a PhD in virology, and I am in my second year of anatomic pathology residency at the New England Primate Research Center. I just recently discovered the TWiM, TWiV and TWiP series of podcasts and they are great! I am training for my first marathon and my long runs were becoming quite tedious with the same old songs on my iPod every day. Now, I download another episode of TWIP every time I head out for a long run and I always have something interesting to listen to. I enjoy TWiM and TWiV, but TWiP is especially great while I am studying for my pathology boards. Although TWiP is based mainly around human diseases, most of these parasites are, of course, also quite relevant to veterinary medicine. Your amusing anecdotes and interesting stories help to cement the life cycles of these fascinating creatures in my head and your entertaining banter makes me feel like I am running with company every time. Thanks for helping the miles fly by and keep up
the good work!

Sincerely,
Lisa

Rufus writes:

Topic suggestion -- I noted recently a news article regarding a treatment method for malaria that addressed the parasite's ability to cause an infected red blood cell to lodge in a capillary and thus avoid being filtered by the spleen. I don't recall you discussing this aspect of the infection. I don't know how effective the treatment is, but the fact that the parasite does this is way cool.

See for example: http://www.ncbi.nlm.nih.gov/pubmed/6348780

Best regards,

Rufus.

Software engineer (for a SEM manufacturer)

TWiP 43 Letters

Robin writes:

http://www.websters-online-dictionary.org/definitions/Trematoda

Trematodes are commonly referred to as flukes. This term can be traced back to the Saxon name for flounder, and refers to the flattened, rhomboidal shape of the worms.

Robin writes:

re men stealing meat from lions

Check out this video on YouTube:

http://www.youtube.com/watch?v=TBpu4DAvwI8&feature=youtube_gdata_player

Liesbeth writes:

Dear Vincent and Dickson,

Let me start thanking you for encouraging me to carry a healthier life. Since taking the bus to the lab each day meant a 20 mins ride, and your podcasts are usually longer, I decided to start waking up earlier and walk every day instead, that way I can hear the whole episode! I feel somehow infected by a parasite podcast that modifies my behaviour in order to be listened, reaching its objective when I arrive at the lab commenting on the last episode heard.

After your discussion on the origins of the term Loa loa, I did some internet research and came up with an article published in 1991 by John D. Ruby and John E. Hall. Since it's really short, I copied it here for you to read:

"It has recently been called to our attention that the word 'loa', used for centuries, first by Africans and later by parasitologists, to refer to the 'eye worm', also appears in the terminology of voodoo or Vodun where it refers to a large pantheon of deities that may possess one's soul or being. The word 'loa' may be derived from the Yoruba word 'l'awo' meaning 'mystery', but according to Bourguignon its origin remains uncertain. To the devotees of vodun, the noon hour, when the sun casts no shadow, is a perilous time. A man without a shadow is a man without a soul and therefore vulnerable to possession by such spirits as 'loa'. To ward of these spirits, believers wear amulets and cast spells. African vodun evolved in Benin, formerly Dahomey, and was brought to Haiti with slavery during the seventeenth and eighteenth centuries. Since both adults and microfilariae of the 'eye worm' Loa are diurnal, with maximum activity occurring at noon (when the West African would be most susceptible to spirit possession), and since West African vodun and the 'eye worm' share a common geographic origin, we have reached the tentative conclusion that one is probably the etymological source for the other. Whether the helminthological Loa predated the anthropological one remains a matter of conjecture"

Knowing something about the origin of the name and the way the parasite was discovered really helps to remember things better!

Looking forward to your next infectious TWIP,
Liesbeth.-

Todd writes:

Found the following reference for the origin of ‘Loa'. Still don't know what it means...

http://books.google.com/books?id=_ktCAAAAcAAJ&ots=q90yrK_QLl&dq=cobbold&lr&pg=PA388#v=onepage&q=loa&f=false

Love the show!

Todd
Laboratory Technician III
Georgia Perimeter College

Peter writes:

Just to get you started <grin>

http://scientiarules.wordpress.com/tag/origin-of-human-tapeworm/

Alice writes:

Drs. Racaniello and Despommier:

Did you see this about Toxoplasma gondii?

http://www.voanews.com/content/feline__cat_litter_bin_parasite_women_suicide/1363046.html

Best,
Alice

Spencer writes:

Dear Vincent and Dickson,

Here is a news piece very relevant for TWIP: The first congenital case of Chagas disease in the US. With a great video from Dr. Jim McKerrow.

Here's the link:

http://www.everydayhealth.com/healthy-living/0706/first-congenital-chagas-case-in-us-reported.aspx?xid=aol_eh-news_17_20120701_&aolcat=HLT&icid=maing-grid7%7Cmain5%7Cdl9%7Csec3_lnk3%26pLid%3D176458

Thanks and keep up the great episodes!

Spencer MD PhD

TWiP 42 Letters

Jesse writes:

Dear Doctor's Racaniello and Despommier,

Huzzah! I have tracked down Dick's missing book on tropical medicine, or at least another copy of said book. While listening to TWIP #40, my ears perked up when Dick said he had lent a book to a student only to have it never return. A quick Google search found the following book: "Tropical Medicine and Parasitology: Classic Investigations" by Mott and Kean of Columbia University circa 1974, which I believe is the book in question. I managed to find a copy online for a steal (less than $10, including shipping!) and immediately ordered it. In 4-14 business days, I should receive a copy, which I will gladly send along to Dr. Despommier as a token of my gratitude for everything that he has done for me (more than you would think).

As a pharmacist working the night shift at a popular chain store, I quickly grew tired of listening to internet radio at night and needed some further stimulation. Your podcast was one of the first that I found and I quickly listened to every episode (multiple times even). This podcast, along with a few other life changing experiences, has inspired me to completely change the direction of my life. I now have solid plans in place to go back to medical school (once my debts are knocked down a bit) with hopes of becoming an infectious disease specialist. My dream is to join Paul Farmer's organization (Partner's in Health) and practice "tropical medicine" while helping those most in need.

Thank you both for all that you do and for taking the time to read my email. If you reply with a mailing address for yourselves at Columbia University, I will gladly send along the missing book once it arrives. As always, looking forward to your next TWIP!

Sincerely,

Jesse

P.S. Are you missing any books Dr. Racaniello? Your work on Polio is greatly appreciated, as my grandfather suffer's from a mild case of Polio myelitis -- so it is obviously a topic that is near and dear to me.

Keely writes:

Hello Dr. Racaniello.

To introduce myself, my name is Keely, and I'm currently a second-year graduate student at UCLA. I work on parasitic worms under Elissa Hallem, and I TA for the undergraduate parasitology class taught by Patricia Johnson.

First off, thanks for all the podcasts. I really enjoy them, and they keep me reading new and interesting papers that are a bit outside of what is usually on my radar. Also, though I was a microbiology major with a strong interest in parasites, my undergraduate institution lacked a full parasitology course, so I learned most of my parasitology from my own reading. I really appreciated the review that TWiP gave me before I started working on parasites for my graduate program.

Anyhow, I was listening to an older episode that I missed somehow, and you mentioned at some point having listeners on the show as a guest. If you ever decided to try and do this, I would love to toss my name in the hat.

Thanks again!

Michael writes:

I am 63 years old. I became ill while serving in Vietnam at the age of 20. For 43 years I have been going to one physician after another physician. Army, VA and private physicians have never been able to identify or diagnose my illness correctly, because every treatment or medicine they gave me, oral and topical, never worked.

For years I argued that I felt that it was parasitic, but the VA eventually convinced me that I had Agent Orange related illnesses and that my idea of parasites was not only futile, but hypochondriasis (a diagnosis, which they actually gave me).

Well, I am still hopelessly suffering. Boils are breaking out all over my body, particularly on my scalp and arms, and I truly feel that I am in late stage severe and chronic Parasitosis, but the VA has me diagnosed with Delusional Parasitosis. Last year in November, I had three very large lesions/boils that grew so large that they burst and left large continuously draining holes (blood and infection) in my arms and scalp. I became interested in the fluid samples and began collecting them in small plastic jars with tight lids. I took these samples to the VA and asked them if they would have them tested/cultured or examined under a microscope to see if anything could be determined or diagnosed. They refused and said that their blood tests for parasites was all that they needed and it had come back negative and there was nothing else that they could or would do. So to date, after years of complaining and arguing, all that they give me in medicines is anything medicines for para
sites.

After that I decided to buy a USB 200x Microscope that could take photos of what I focused on underneath the lens, which I found on Amazon marked down from $250 to $80, and examine these fluid collections on my own.

Doctors Racaniello and Despommier, both of you may be surprised. Thus far (for five months now), I have 200x photographed over 3000 pictures of the fluids draining out of these non-healing, large, deep, open wounds. In January I took nearly 1000 (what I had at the time) of these photos to the VA and they wouldn't even look at them, notwithstanding that they also did not give me the necessary wound either. If what I am looking at in the photos is correct I am acutely infected with millions of nematode microfilariasis worms and flukes.

Lastly, is it possible for me to send you a few of these photos (I can send them all on a snailmail CD or post them to Photo Bucket) for you to look at and advise me on whether or not they are in fact one or more of the over 10,000 nematodes that find humans as a desirable host? Thank you.

MICHAEL

Raphael writes:

Good day, doctors!

I did not hesitate to write this right after hearing an email you responded on TWiP 39.

I would like to thank cytotechnologist Rebecca for the comment and Dr. Dickson for additional info on how tricho is vertically transmitted from mothers to female newborns and how it could be lifelong. Not so many years ago, I had a rare opportunity to have a urinalysis result that came out which was trichomoniasis positive. The female patient who was in her early twenties denied that she had no history of sexual contact but knowing very well that tricho is a sexually-transmitted infection, I immediately did not believe her. Nevertheless, I treated her with an antiprotozoal drug.

Now that I know how tricho is transmitted, I owe that patient my apology.

Tracy writes:

Dear Dick and Vincent (if I may be so bold)

I listened to the podcast on Leishmania this week as I prepped for my Leishmania lecture in Medical Microbiology. I was struck by Dick's comment that we didn't know what the amastigotes ate while in the the phagolysosome. I ran across an annual review in Microbiology article that seems to address the metabolic needs and potential nutrients sources for the amastigotes while in the phagolysosome.

Here is the link to the paper: (Metabolic Pathways Required for the Intracellular Survival of Leishmania)

http://www.annualreviews.org/doi/full/10.1146/annurev-micro-090110-102913

I enjoy your podcasts and my students find them an easy way to approach very complex material.

Keep up the good work!

Laura writes:

Hiya

Firstly i would like ot say that I followed your podcasts whilst i undertook my undergraduate degree and found them useful for revision. I am now starting a PhD at Nottingham University in England this year and would like to thank you for providing such great podcasts.

I would like to ask, as mosquitoes carry a number of parasites/viruses that can infect humans, is it possible for one mosquito to infect a person with more than one species of parasite/virus, for example could a person get malaria and leishmania at the same time from the one mosquito?

Jon writes:

Ok, this sounds rather unpleasant - but may explain a lot about the world :)

The Brain: Hidden Epidemic: 
Tapeworms Living Inside People's Brains

http://discovermagazine.com/2012/jun/03-hidden-epidemic-tapeworms-in-the-brain

Suzanne writes:

Maybe Dickson knows or maybe someone else has mentioned it, but if you wash immediately with soap and cold water poison ivy oil washes off more easily. Wash all your clothes in cold water, too. Most people want to wash with warm water (I've done it and boy did the rash spread) but cool or cold water works best. I hope his has cleared up by the time you get this!

http://www.poison-ivy.org/html/faq.htm

Jim writes:

Dickson mentioned this 2 vol set on the last TWIP. This link from the World Catalog may have specifics as well as where Vol 1 can be bought, and cheaply too, it appears. Volume 2 is in the World Cat database, but not linked to any library or store -- odd. The key thing is that the title may be "Tropical Medicine and Parasitology." I found a few Vol 1's, but no Vol 2. Might a Vol 2 be sold as a Vol 1? Here is a link that at least shows a Vol 1 & 2 set has at one point been listed on Amazon, so a periodic search may eventually be productive. I like looking at old books and will keep an eye out in this area. Maybe a mention on a TWIP would alert listeners to also watch for the set. Not an expensive item.

Ref deer flies: I have used these tred-not patches on my hat for several years and they are quite good. Just ordered another big batch. Sound like something Dickson can use, if he is not doing so already. Only negative aspect is that they are only good for one day. The last of my current batch were purchased last year and worked just fine.

Beautiful shows, all!

Regards,

Jim
Smithfield, VA

Stephen writes:

This is actually for TWIP, FYI

(BTY: It's not easy to send to TWIP from the Nature app, because it assumes an email address.)

Bovine TB disguised by liver fluke

Nature News,Published online: 22 May 2012; | doi:10.1038/nature.2012.10685

http://dx.doi.org/10.1038/nature.2012.10685

Meika writes:

Hi,
I have been one of your silent fans for about a year now. And I absolutely LOVE all three of your podcasts. I am a scientist at heart and by training, but a horse trainer for my living. I recall a past podcast about a dressage barn that had an outbreak of toxoplasmosis, and I am trying to search for it, but Toxo is a very popular subject for you guys and the list is long. While I am willing to listen to all episodes again to try to find it, can you give me a hint as to which episode it was and shorten my search?

Your show has been an inspiration to me as well as a fun link to my past life as a scientist. Listening to TWIM has prompted me to go on a several days long spree of reading reprints of my grandmother's research. The earliest I can find is 1933 and she is the lead author in The Journal of Bacteriology on carotenoids and Vitamin A in bacteria. She also was interested in nutrition and color inheritance in Serratia and published her research for four decades. I cant help but think that she was the rare woman bacteriologist who led by example for so many women back then. Thanks for keeping me interested and helping me know my grandmother better.

Meika (pronounced: mica, like the mineral.)


Mike writes:

Doctors, I have recently discovered podcasts and the very first thing i searched for was one on parasites. I have been fascinated with parasites since grade school and will be starting my 3rd year of veterinary school in the fall so i was very excited to see a podcast devoted entirely to parasites. granted a bulk of my parasite knowledge is animal based i still very much enjoy TWIP. I started your first episode and have just finished the episode on hookworms. a few things i wanted to bring up, from the perspective of a budding veterinary parasitologist. First: A. caninum is capable of infecting young pups through the bitches milk and i was wondering if any human hookworms have been documented to do the same, at least for animals, hookworms can also be contracted from eating the 3rd larval stage (generally pica) or from eating a variety of other animals that have eaten the 3rd larval stage and act as a transport host, for example, the larva eaten by a mouse, enter a hibernation state until a cat or a dog comes along and eats the mouse. as the tissues are digested away the larva wakes up, receives environmental cues and will proceed to develop to an adult, this is a particularly common way for cats to contract A. tubaeforme given their predatory nature. Has anything similar ever been documented with a human species of hookworm?

Second: as to the question of prevalence of hookworms in dogs in America, yes they are definitely endemic. they are a problem across the nation especially shelters, as are whipworms, ascarids, coccidia.

Third:in one of the episodes on tapeworms Dick was comparing the pork tapeworm to the beef tapeworm and mentioned that horses and cattle are different from pigs and asked Vince why. Dicks answer was that cattle and horses are both ruminants while a pig is an omnivore, i don't know if this was an accident but horses are not ruminants, they do not have the complex foregut fermentation system of cattle, sheep, goats, deer and other similar animals, horse are hindgut fermenters and do a bulk of their digestion in their colon and cecum (appendix) but both are herbivores.

and last, to Vince, you had peppered Dick with questions on what animal species had what nematode parasites, to broaden your list, ascarids are found in horses, pigs, cats, dogs, cattle, raccoons, wild canids and felids and mostly any other mammal. hookworms are just as ubiquitous in the animal kingdom. that's all for now but i am sure i'll write again, keep up the great work gents. I understand that this is a long email and will not be offended if you don't read it on the air but i would greatly appreciate answers to my questions if possible

Mike writes:

Doctors, again i write to you because a few things had slipped my mind while writing my last email. Dick, when you were describing the live cycle of ascaris, you said there was an operculum on ascarid eggs. I have seen many ascarid eggs of several species and have never seen an operculum, did you miss speak or have i just not been looking carefully enough? or is it specific to A. lumbricoides? it has always been my understanding that the operculum is rather rare in nematode species save members of the orders Oxyurida and Enoplida. speaking of Enoplida, one of the many parasites we discussed in parasitology course that is part of our curiculum was Dioctophyma renale, the giant red kidney worm, a particularly cool video of a dog in canada that was unfortunate enough to contract a few of these has been posted on youtube: http://www.youtube.com/watch?v=5ob6S_EqR1c Has there ever been a case in people that you know of?

Lastly, I just listened to the Strongyloides podcast, do pigs, like people often pass the larva in their stool but in the veterinary world they are the exception, most of the time in other domestic species the eggs are what are passed does this ever happen with people? have you ever seen a Strogyloides egg? This particular parasite can be debilitating and even fatal to piglets but with the modern management techniques in the American swine industry the problem has ceased almost entirely.

thanks again for humoring me gents,

Daniel writes:

Hello again professors. Sorry to keep bugging you but I thought you might be interested in a website I happened upon the other day.
It's a crowd sourced pseudo-video game designed by UCLA students in which the players have to identify RBCs that are infected with malaria. Here's a bit of text from the site and the link.

The Idea

Analysis and related diagnosis of medical images, regardless of the source and imaging modality, are tasks that require a great deal of expertise, demanding significant training of medical practitioners prior to being able to accurately interpret and diagnose such images. This is particularly true in analysis of microscopic data, creating challenges in resource-limited settings and developing countries, where properly trained health-care professionals are difficult to find.
Results

We have shown that by utilizing the innate visual recognition and learning capabilities of human crowds it is possible to conduct reliable microscopic analysis of biomedical samples and make diagnostics decisions based on crowd-sourcing of microscopic data through intelligently designed and entertaining games that are interfaced with artificial learning and processing back-ends. We demonstrated that in the case of binary diagnostics decisions (e.g., infected vs. uninfected), using crowd-sourced games it is possible to approach the accuracy of medical experts in making such diagnoses.
Specifically, using non-professional gamers we report diagnosis of malaria infected red-blood-cells with an accuracy that is within 1.25% of the diagnostic decisions made by a trained professional.

http://biogames.ee.ucla.edu/

Wish you both the best and let me apologize again for bombarding your inbox.

-Dan

Nick writes:

Hi Vince and Dick,

I'm writing hoping that you haven't already been flooded with emails regarding heartworm treatment and resistance, and if you have, maybe I can still be able to contribute something. I'm a dual degree student (DVM/PhD) at Cornell University and am studying the mosquito vector ecology of heartworm. As your listener mentioned, there have been some reports of persistent microfilaremia in dogs on preventive medication. Some of the issue has been shown to be due to lack of owner compliance to administering the drug, but, after controlling for that, there have still been dogs identified with persistent microfilaremia despite preventive treatment (and often after the adults have been killed or removed), which currently is the only standard method to clear the microfilariae.

This loss of efficacy is specifically of the microfilariae to clearance, not to melarsomine (Immiticide), which is the adulticide. Ivermectin formulations used as preventive/microfilaricidal, which you eventually got to during the podcast anyway! There have been some studies looking into the effect of doxycycline on clearing the Wolbachia in Dirofilaria immitis to reduce their ability to reproduce and possibly reduce microfilarial and larval fitness for transmission, which I thought you'd find interesting.

I'll be sure to chime in on veterinary topics or, more likely, vector biology topics that I know something about. Also, I was surprised to hear that Dick came to speak at Cornell but I had missed it! I'll have to be more attentive next time,

Keep up the good work!

--Nick

Alberto writes:

Dear Parasite Hosts

I thought Dickson would be interested in this story from ABC's ( Australian Broadcasting Corporation) Science Show which features an interview with A/Prof Daryl Davies of USC, LA in which he discusses the potential use of ivermectin for the treatment of alcoholism.

http://www.abc.net.au/radionational/programs/scienceshow/drug-shows-promise-as-treatment-for-alcohol-abuse/4006394

I found this recent article in Neuropharmacology from the Davies lab which describes the experimental use of ivermectin in a mouse model of alcohol dependence.

http://www.sciencedirect.com/science/article/pii/S0028390812001128

I quote from the abstract:
".. in the present study we investigated the effects of this agent on several models of alcohol self-administration in male and female C57BL/6 mice. Overall, IVM (1.25-10 mg/kg, intraperitoneal) significantly reduced 24-h alcohol consumption and intermittent limited access (4-h) binge drinking, and operant alcohol self-administration (1-h)."

The mechanism of action appears to be via antagonism of a purinergic receptor in the brain. In the interview, A/Prof Davies explains that in parasitic organisms, the drug acts by stimulating an excitatory glutamate chloride channel.

Keep up the great work guys.

Cheers

Ethan writes:

I found this in the mail today from my water department and had to forward it to you guys. Looks as though my city's water is clean of any cryptosporidium parasites, but inhabitants of other middle Tennessee areas perhaps are not so lucky!

Enjoy,

Ethan

Peter writes:

Watching 'Origin of Us' a BBC2 documentary on Human evolution. In Episode 2, 'Guts', the host Dr. Alice Roberts mentions an interesting hypothesis relating to tape-worms.

It appears that the lion tape worm and human tape worm are very closely related. A DNA study is used to determine the time of worm
speciation. It is assumed that around this time Humans began to steal or eat the same prey as lions. Eventually the worm evolves from Humans as the intermediate, host to becoming the definitive host. Aprox 800k to 1.7 million BCE.

She does not cite the work but, being a BBC doco, I have no reason to doubt the work is genuine and published somewhere.

Novel idea to use tape worms for plotting meat eating in Humans.

Stephen writes:

Might this help to learn how Cobbold named Loa loa.

I don't have access to the journal, and didn't find the full text on line. Pubget.com finds it at the nearest university library, but it's $31.

A bit steep just to satisfy curiosity.

http://www.ncbi.nlm.nih.gov/pubmed/15463486

Rohit writes:

Dear Dr Racaniello and Despommier,

I listened to the fascinating episode Worm in the Eye: TWIP 40 earlier this week. Coincidently, I saw this news story, about a doctor taking out a worm from the eye in Mumbai India today. http://www.bbc.co.uk/news/world-asia-india-18640495

I thought that it would be interesting to hear Dr Despommier's expert comments about this worm/case. I apologize if this worm has already been discussed and I have just not caught up with that particular episode yet. I am curious if the doctor's statement "The worm could have travelled deeper into the eye or gone to the brain through the optic nerves, which could have been fatal" is true. Being a virologist, I know that Polio and other viruses travel through nerves but can worms too travel through nerves?

Dr Despommier is a wonderful story-tellier. I find it amazing how much he can remember. Keep the awesome worm-stories coming along with some very intriguing questions from Dr Racaniello.

Thanks

Rohit

Rohit
Postdoctoral Fellow, Chandran Lab
Department of Microbiology and Immunology
Albert Einstein College of Medicine

Ethan writes:

Dickson mentioned how crazy it is that ticks can live up to 50 years in a dormant state without taking a blood meal, and it immediately reminded me of this:

http://m.phys.org/news/2012-03-survive-subjected-electron-microscopy.html

After subjected to conditions within a vacuum and being blasted by an electron beam, the tick keeps ticking.

Joann writes:

Hi Drs. Racaniello and Despommier,

Great podcast!

I'm a graduate student in epidemiology and am completing my practicum this summer working as a health inspector in a coastal town. One of the health agents I work with used to be a chef at one of the local restaurants. She described to me (warning this is gross!) how they used to use tweezers to pull out little (~1" worms) from cod and swordfish that would later be cooked in served. My questions for you: do you have any idea what these worms might be? And, is there any chance that ingesting them (or perhaps eggs/larvae, etc.) of these worms would make humans sick? Regardless, still pretty nasty.

Keep up the great podcasts! I have a long commute usually and have been twipping and re-twipping.

Best,
Joann

TWiP 41 Letters

Adam writes:

What up Doc's?

I'm writing to voice my complete disagreement with the sentiments of Sven Urban, in his letter on TWIP 38, that you as hosts are prone to engage in a ‘degree of banter which is distracting'.

I'm sure Dickson does not mind being antagonised, and like caffeine on an adenosine receptor the antagonist in this case is a vital educational aide serving to keep me awake.

I will concede the point that Dr. Racaniello is most often the instigator of these disruptions. For negative control please see the first episode of TWiV which Alan and Rich did on their own - this ran disappointingly efficiently, depriving me of company and edu-tainment on the latter half of a long commute to uni.

I will end with this observation - If you can't play as a child you can't be imaginative, and the last thing we need is scientists without imagination (I believe these are called accountants btw).

You have this listener's permission to be as disruptive as you like.

Keep up the good work.

Regards,
Adam,

Melbourne, Australia (26◦C Partly Cloudy)

PS: Consider this my vote against This Week in Old Guys Reading Aloud from Textbooks (TWiOGRAFT).

Liesbeth writes:

Hi Vince, hi Dick,

my name is Liesbeth and I'm doing my Masters in Epidemiology of Infectious Diseases at the Oswaldo Cruz Institute in Rio de Janeiro. I started to follow TWIV a couple weeks ago and now I'm absolutely infected with TWIP and trying to catch up with TWIM also. Thank you so much for this great initiative, for the effort and time dedicated... it's really appreciated and highly contagious! Keep up with the amazing podcasts!

All the best,
Liesbeth.-

Bill writes:

Dear TWIP Team

30-40 years ago, completely unaware of giardia, when hiking in the high Sierra we commonly drank from mountain streams without any ill effect. There's much greater awareness since then, but I wonder if the risk of infection has really increased. i.e. if drinking from fast flowing streams at high elevations is as safe now as then. What do you think?

Arthur writes:

Vincent Racaniello and Dick Despommier- you guys are GREAT!

I discovered TWiP one month ago and so far I'm addicted, I don't know how many guys in Kenya listen to TWiP, I suspect I'm the only one but I'm quickly spreading the gospel. I have special interest in the Leishmania podcast because I came across so many patients with Kala Azar especially during my internship year in Eldoret- Kenya, last year and I have seen firsthand the kind of clinical havoc the parasite can cause. A few questions;

Q.1 Why do patients with Kala Azar have pancytopaenia (most of the time-severe)?

Q.2 Apart from the BMA and the Splenic Aspirate, what are the rapid diagnostic methods that are available and how accurate are they?

The doctors working for MSF- (doctors without borders) in Pokot (an area near the border of Kenya and Uganda where Visceral Leishmaniasis is endemic) usually base there diagnosis on epidemiology and some rapid testing methods and start treatment- mainly sodium stybogluconate but some patients still die, so over to my next
question;

Q.3 How effective is Sodium Stybogluconate in the treatment of Kala Azar? Is Amphotericin B more effective despite its side effects?

Observations: In Kenya the most vulnerable age group are kids around the age of 6 years because they play on and around anthills where most sandflies are found.

Prof. Despommier and Prof. Racaniello, it would be really great if you give lectures in Kenya- the medical fraternity here will really appreciate it.

Dr. Arthur Mumelo - Medical Officer - rural Kenya.

Roxanne writes:

I am a new to the podcast. I gotta say I love the repartee. I find it entertaining and it sometimes adds suspense when the answers are put off for a bit. As a disclaimer: I am an avid CarTalk fan. Your names, Vincent and Dickson, even share an alliteration component like Click and Clack.

Roxanne, PhD (microbiologist turned forensic DNA analyst, public health hopeful)

Daniel writes:

In the early TWiP episodes Dr. Despommier spoke at length about neurocysticercosis. You mentioned that the neurocysticercoses remain dormant and the patient is asymptomatic (unless they obstruct the foramen magnum) until they begin to die and subsequently cease to suppress immune response. My question is, if cestodes that become neurocysticercoses have no aging genes what causes them to die? Is it because they are encysted and can't gather nutrients from their environment?

-Daniel

Ethan writes:

I had some questions regarding the difficulties in producing successful vaccinations for sleeping sickness. From my understanding, the biggest trouble is that as the immune system produces effective antibodies and the trypanosome populations decline in the host, gene expression for molecules making up the outer VSG coats in progeny switch to quiet genes thus changing the VSG molecules and rendering the host's current antibodies useless.

Why can't we vaccinate against all of these antigen variations? If that is impractical, why can't we vaccinate against every 10th antigen, for instance, so that once the trypanosomes turn on one of those genes it is then eliminated by the host's inoculated immune system? I'm also curious as to whether the trypanosomes always begin their life in their host with the same VSG or if it will vary its first expressed gene. If it always starts in the same place and uses a specific order, we could better select which ones to make vaccines for.

Since the trypanosomes switch these genes in a specific order, can we catalog this progression in a model and use that as a reference for the host's current and future antigens? If so, we could select the most relevant vaccine that would be effective against the impending wave of new antigens, playing the waiting game for our target gene to be switched on.

Sorry for the barrage of questions, this just had me thinking the last few days. It seems to me a vaccine for this should be easy - but obviously I'm wrong!

Christopher writes:

Hello gentlemen,

First off I want to thank you both for an excellent and entertaining podcast. I am a regular listener and I enjoy your show immensely!

I had a question regarding the "successful systems attract parasites" quote. Dr Despommier mentioned that this quote had won a poll in which readers were asked to vote for the pithiest statement on biological research over a specific time frame (or perhaps that best summarized 100 years of biological research??). As part of an upcoming faculty lecture series, I am preparing a talk on how- at least from an ecological perspective- that statement is perhaps better worded this way: "parasites play key roles in defining and structuring successful systems". Unfortunately I have been unable to find any reference to this poll online, and I was hoping Dr Despommier would be so kind as to remind me of the details (what publication / organization ran the poll, what were the "rules" of the contest).

Thanks for your time, and I look forward to more awesome podcasts in the future!

Cheers, Chris
____
Christopher Blanar, Ph.D.
Assistant Professor of Biology
Division of Math, Science, and Technology
Farquhar College of Arts and Sciences
Nova Southeastern University

Stephen writes:

Re: using sari cloth to filter water to reduce cholera, the agent being filtered is a copepod that carries Vibrio, not the Vibrio itself:

"In laboratory experiments employing electron microscopy, we found that inexpensive sari cloth, folded four to eight times, provides a filter of [about] 20 [microns] mesh size, small enough to remove all zooplankton, most phytoplankton, and all V. cholerae attached to plankton and particulates [greater than] 20 [microns]."

Links to download a pdf of the PNAS paper:

Reduction of cholera in Bangladeshi villages by simple filtration

www.pnas.org/content/100/3/1051.full.pdf

PS, I wrote to TWIV previously regarding hyperparasitoids. I really like the extended format and the fact that your shows feature people who know what they're talking about, or admit that they don't know.

Chris writes:

Hi Vincent and Dickson,

Love all your podcasts. I just wanted to write in to clarify a couple of things that were said on a couple of recent TWiP episodes.

On TWiP 37, you were discussing the use of water filtration in the eradication efforts of dracunculiasis and the subject of the work of Rita Colwell came up in which she used sari filtration to filter out Vibrio cholerae from natural water sources. Dickson mentioned that this might be possible due to V. cholerae's long flagellum. In fact, the reason this is possible is because of V. cholerae's affinity for chitin. The majority of the bacteria in nature tend to be attached to small crustacean zooplankton. It is this property that allows enough of the bacteria to be filtered out of the water to significantly reduce the incidence of cholera in areas using this practice. Thus, because of this, I think it would be unlikely that this type of filtration would have a significant effect on removing other enteric bacteria (infectious dose playing a role here too).

On TWiP 38, you were discussing Babesia, and Dickson said that the species was bigemina. However, B. bigemina is vectored by Boophilus ticks and is the cause of a cattle disease known as 'Texas cattle fever' or 'red-water fever'. In the United States, Babesia microtiis the species that most commonly infects humans and is vectored by Ixodes scapularis ("deer tick") in the U.S. Northeast and New England regions. Thus, it is B. microti that is the most important species infecting humans and has a deer tick - rodent life cycle. Since it infects red blood cells, it has also been an issue for the blood supply.

Just wanted to clarify those two statements not as criticism, but more to get the correct information out there. Keep up the great work with the podcasts.

Chris Whitehouse, Ph.D.
Microbiologist working for the U.S. Government in the Washington, D.C. Area

Allan writes:

I've enjoyed listening to your your TWIP podcast. You are a wonderful story teller and though I have worked in this field a bit as well, I never fail to learn something. I listen in the car on Sticher. My kids think I'm weird but they like it too.

In the episode today (1MAR12) on dracunculosis, you made the natural connection that we come across cyclops both in Malaria and the Guinea Worm programs, but in the podcast you mentioned a couple times that mosquito larvae eat the cyclops and I think you probably meant the converse.

We add cyclops to family water jars in Cambodia to eat the mosquito larvae.

Anyway keep up the great work. You strike a nice balance of making the field more approachable, while keeping it interesting enough even for the grouches.

Best regards,
Allan

Allan, DIH, MPH
make-it-count.com

http://www.gerrymarten.com/publicatons/pdfs/GM_Copepod-Production.pdf

TWiP 40 Letters

David writes:

Love the TWIPs! I'm a helminth user (25 hookworm for allergies), so your programs are fascinating!

Dr. Racaniello, after listening to TWIP 33 where you were discussing the history of your surname, I thought I'd give your listeners a way to remember your obviously Italian name. It's the rap song, "Black and Yellow"! lol http://m.youtube.com/watch?v=ITKR7OZyyOw

Peter writes:

Dear Professors,

please do NOT abandon your style ever!!

Even if there´s some privacy in there, be it fishing or simply the weather or whatever, it has to be in your podcasts because it makes them authentic! Even more, talking like you with so much commitment and humor increases the amount of learning. You know why!

I always tried to be a good teacher (for natural sciences at a german secondary school since more than 20 years) exactly in that way.

And scientific researches in recent years have approved that the brain of all ages learns better and more effectively with these attributes.

Here you are:

http://med.stanford.edu/ism/2012/january/reiss.html

And remember, just because informations cause an emotional response, and that means attention is rising:

http://spots.wustl.edu/SPOTS%20manual%20Final/SPOTS%20Manual%204%20Learning%20Strategies.pdf (page 7)

Please keep on with your excellent work, you are pioneers in science and "face-to-face" interaction,

greetings from Wiesbaden, Germany

Peter

Richard writes:

Dear Dickson & Vincent,

I recently delivered a lecture to nursing students at Kilimanjaro Christian Medical Centre in Tanzania, on a subject that Dickson is very well versed, Trichinella.

I am a medical entomologist & have no knowledge of Trichinella so quickly had to do some reading & piece together a basic lecture. In the process of preparing the lecture I was left with a couple of questions that I hope Dickson can answer.

1- In East Africa the species is Trichinella nelsoni. My understanding is that there have been very few human cases reported (200 ish) and they have all come from eating undercooked warthog or bushpig. In Tanzania pork meat from domestic pigs is commonly eaten (on a sidenote pork is called "kiti moto" in swahili which translates to "hot chair"- possibly because if the muslim population of Tanzania get caught secretly eating pork they will be in big trouble, or the "hot chair"). I was left wondering why T.nelsoni has not made the jump from wild pigs & warthogs into domestic pigs? I was asked this question in class & my explanation was that it could be due to geographic isolation as warthog/wildpigs are mainly found in "bush" and savannah areas where usually only Maasai are found. Maasai keep cattle, sheep, & goats but not pigs. Do you think this is a reasonable explanation? How would you have answered this question?

With rapid population growth in Tanzania do you think expanding human settlements could result in T.nelsoni making the jump into domestic pigs & resulting in more human cases?

2- In the excellent book, "Manson's Tropical Diseases" the T. nativa Arctic cycle shows that polar bears can become infected by eating raw walrus and seal meat. How do seals & walrus become infected with Trichinella? Don't they eat fish??

Looking forward to hearing your thoughts.

Best wishes

Rick

Ethan writes:

Hello Vincent & Dickson!

I'd like to thank you for the superb podcasts I've had the pleasure of enjoying the past few months. Not having any previous interest in parasitism or virology, I downloaded a few podcasts on a whim and was immediately hooked!

Working a night shift by myself in the biology building of a university calls for TWIx podcasts for entertainment. While listening to the TWIP episode on hookworm, I noticed a ton of slides left out in a lab room full of microscopes. What was on them? Parasites galore! I took the liberty of finding slides of hookworm in various stages of life cycles and got to follow along during the podcast and actually see with my own eyes real examples of what you guys were describing under the microscope - it was awesome!

I really enjoy being able to learn from TWIP being a non-scientist (although I'm going back to school for physics soon, so I'll be in the scientist club before too long!). I have to look things up to follow along in TWIV, and TWIM is often way over my head, but I try to listen to both anyway, they're always interesting.

I've since listened to all the TWIPs and would love to hear new ones added more often. I'm also looking forward to adding Dickson's new book West Nile Story right beside my recently read copy of Parasite Rex.

Sorry for the lengthiness of the email!

Thanks again,

Ethan

Rebecca writes:

Hello again!

Thanks for mentioning my email and talking about my obscure profession! Cytotechs screen pap smears in addition to fine needle aspirations from just about any body site. A normal day consists mainly of screening pap smears, but I get to look at some lung, thyroid, liver cells, etc. Legally, we have to screen all paps at an appropriately licensed lab with a Medical Director and Technical supervisor. Gone are the days of screening in your underwear at home - well, maybe some folks can still screen in their underwear. My workplace frowns on it. A lot of slides are still hand screened on an regular ol' light microscope, but some are analyzed by a computer first. All slides must be screened by a cytotech or pathologist - the computer just uses an proprietary algorithm to determine the areas of the slide with the highest probability of abnormal cells to guide our screening. We send all slides we diagnose as abnormal to a Pathologist. He or she has the final say. In addition to looking for cancerous or precancerous cells, we also look for non-cancerous infectious agents. HSV and CMV produce some gorgeous cells! Check google images for HSV or CMV cytology. As far as it being kind of boring to just look through the scope all day: you have do enjoy looking through the microscope or you will quickly go insane in this line of work. I personally enjoy the focused attention this work requires - it's like mediation for me. Bonus: I get to listen to awesome podcasts while helping people. Additionally, the microscope rarely back-sasses me, so I don't have to deal with any workplace conflict or stress:) Thanks again for the podcast!

Dan writes:

Hello gentlemen,

My name is Dan and I'm considering becoming an epidemiologist. I discovered your podcast and am slowly working my way through all of the old episodes, which I find extremely fascinating.

Forgive me if it's already been mentioned on your show as I am listening to the episodes in chronological order and haven't yet arrived at the most recent one, but I would like to recommend a book called "Parasites: Tales of Humanity's Most Unwelcome Guests" by Rosemary Drisdelle. I recently purchased this novel and it served as a wonderful primer for your show and as fantastic "thought food".

If you can recommend any books, journals, or other publications that would be helpful for a budding young student such as myself I'd be very appreciative. I know for certain that with my next paycheck I'll be buying Mr. Despommier's "Parasitic Diseases".

Thank you very much for publishing this podcast! I'm very happy that I've discovered it.

Best regards,
Dan

TWiP 39 Letters

Rebecca writes:

Thanks for the podcast! I wanted to add a quick note about diagnosing trich: As a cytotechnologist, I diagnose trich on pap smears daily. I imagine this is how a lot of ladies find out they are infected.

Peter writes:

Greetings Dickson and Vincent,

A cool overcast 14°C on the Eastern Mediterranean coast of Turkey as I write this.

Further to my question on a previous TWiP about whether parasites that used blood feeding vectors made the host more attractive to the vector I found this article on parasitic manipulation in vector-borne diseases
http://www.biology.emory.edu/research/deRoode/PDFs/lefevre%20and%20thomas%202008.pdf

and this 2005 study which indicates that malarial parasites do manipulate their mosquito vector's biting behaviour:
http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0030298

The results look positive even if the number of test subjects was very small.

It was a slow day at work so I had time to spend on the internet.
A couple of other interesting items I found:

A new genus and species of leech (Tyrannobdella rex) from Perú that feeds from the mucous membranes of animals and when feeding may remain in situ for several days.
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010057

Blood feeding by proxy - the East African jumping spider Evarcha culicivora feeds on vertebrate blood by preferentially preying on blood-fed Anopheles mosquitoes. Not a parasite, but still interesting.
http://www.ncbi.nlm.nih.gov/pubmed/22032682


I would also like to suggest a possible guest for TWiP - Dr James Logan of the London School of Hygiene and Tropical Medicine http://www.lshtm.ac.uk/aboutus/people/logan.james

Cam writes:
I'm an amateur at this, looking for some help with identification.

These are in human blood, drawn at night via pin prick to the finger, magnified about 300x. No additives or stains. Nocturnal periodicity, so not found by any more conventional method. Note the thin, translucent folds on the head shot, and "V" shaped head. I was thinking mansonella, but the egg and nocturnal periodicity are not right for mansonella. M. perstans has a ribbon-y quality at the tail, not mid-body.

Warm regards,
Camden

p.s. Also wondering what the object is at 3:00 on the tail shot.

Peter writes:

A POTENTIALLY lethal tick infection newly identified in Australia has mysteriously emerged on the NSW south coast.

Doctors have revealed the first reported Australian case of human babesiosis, a tick-borne infection that carries a 5 to 10 per cent
fatality rate, higher than the death rate from the most common tick bite infections.

The victim was a 56-year-old man from the south coast who died, it is thought, partly as a result of babesiosis. In a report published today in the Medical Journal of Australia , doctors say the infection probably contributed to his death from
multi-organ failure last April.

The report of the first babesiosis case in Australia thought to have been locally acquired had raised ''intriguing questions'' about how
the infection is spread in Australia, the lead author of the report, Sanjaya Senanayake, of the Australian National University, said.

http://www.smh.com.au/national/origin-of-lethal-tick-infection-a-mystery-20120318-1vdse.html


Todd writes:

I was reading news from my childhood home, Louisiana, and saw an article about a surge of heartworm infection due to a warmer and
wetter than normal winter (more mosquitoes roughly translates to more heartworm infection). The more concerning statement though: "...even more disturbing is that some dogs, who have been on a heartworm preventative medication, are still coming down with the potentially fatal disease."

Can you offer some details and insights into what the vets are seeing?

http://www.theadvertiser.com/article/20120327/NEWS01/203270332/Local-vets-stumped-by-heartworm-mystery?odyssey=mod|mostview

Peter writes:

Hi TWiP Team

I was talking to someone about animal diseases and they sent me these links to some plush parasites:
http://www.giantmicrobes.com/us/main/menageries/
http://www.giantmicrobes.com/us/main/critters/

Francois writes:

Hello Monsieur Despommier & signore Racaniello,

Before all, sorry for my English.

And It's now 24°C in southern France. First water restrictions of the year.

My research years were oriented toward food safety & ferments selection. A.k.a fight Listeria in food with other bacterias or cheese ecology.

During, this previous life, I've found some some pretty amazing cheese ferments.
And I'm quite sure the smell & taste of some the resulting raw milk cheeses would have killed mosquitoes & parasites alike.

I've recently listen the oldest TWIV & TWIP, with exceptional pleasure.

I would like to follow on Mr Despommier comment about the 1981 movie "Quest for fire" (TWIP 10), & his suggestion that parasites exchanges between modern & Neanderthal humans could have been deletarian for Neanderthal.

Now, with proof that those populations have reached a certain level of intimacy, at least in the case of the Denisovans. What kind of traces paleoparasitologists could find to conclude that this was indeed hapenning : traces on bones, eggs or cysts in caves soils.
Or at the, now available, genetic makers. Ex : drepanocytosis ?

There was also wooly mammoth, sabberthoot tigers & bears in the film.  Do people get parasites by eating elephants, bears or big cats meat, because it seems that the megafauna was a frequent part of the neandertal diet and vice versa ? I've also heard about tiger, bear or canid meat in today asia, & seen hippopotamus meat in Togo.

Other info, I was 7 in 1982, when I & most of my school mates saw the film. No one have been bothered by the most "X-rate" part, but we were freaked by the "eat people alive bits by bits" part. Today the film is frequently seen in school by children between 7 to 10 years old.

Best regards, & all my thanks for those amazing efforts.

PS : For Mr Despommier
La Tour vivante (The Living Tower)
http://www.agritecture.co/post/12878007672/the-living-tower-a-building-that-weaves-agriculture#.T3sIP458zIo

TWiP 38 Letters

Carlos writes:

Dear Vincent Racaniello and Dickson Despommier

I am an avid listener of TWIP since its start, have been following TWIV for at least two years and, surprise, also follow TWIM.

My field is Computer Science, but I crave for information in all areas I find interesting. To me, your Podcasts fill the role of giving me very pleasurable conversations on science, and help me better understand fields where nature dictates the validation of the research. In my field we often deal with the construction of artifacts that are validated by their mathematical properties, so the validation process is a bit more detached from nature.

What prompted me to write now is that I just listened to the start of TWIP #33 and your discussion of how google scholar indexes more publications and citations, than PubMed I believe, but with a more messy presentation format. You might find it useful to explore a new tool that allows authors to aggregate papers in a specific google scholar author page. After one does that, its easy to sort by publication year, impact etc.

Take a look at the following link to see how to set it up:

http://googlescholar.blogspot.com/2011/11/google-scholar-citations-open-to-all.html

The strong point is that the tool makes it very easy to identify (and separate) your own publications and co-authors. For more common author names its often a mess to separate one's articles from the other homonymous authors.

Keep doing the excellent job of promotting interest in your fields and science in general.

Regards,

Carlos

Universidade do Minho,
Portugal

Peter writes:

Just watching a clip on cellular metabolism. The background music was the Vangelis theme from Carl Sagan's Cosmos.
A very great sadness came over me.
He looked outward whilst you guys look inward, so to speak.
I feel sure he would have loved your shows and would eagerly have embraced podcasting.
You are doing the inner Cosmos.
Thank you.

Emerson writes:

Hi guys,
love the show.

You listed csf, blood, lymph, urine, aqueous humor, cochlear fluid, and semen.

I'd like to suggest that there are more than 7. Ignoring interstitial fluid and cytosol I'm fairly sure that bile is sterile before it leaves the gall bladder. Synovial fluid within the joints is typically sterile, the falopian tubes/ ovaries are bathed in sterile fluid. Tears are also sterile before they are cried I believe.

Take care!

Laura writes:

Drs. Vincent Racaniello and Dickenson Despommier,

I'm a Masters student doing research on Soil Microbes, and in my research have come across papers referencing Mollicutes (phytoplasmas, mycoplasmas, ureaplasmas, and spiroplasmas are examples). Are there any parasitic mollicutes that actively infect humans and cause detrimental symptoms?

Thank you,
Laura
Chatham University

Peter writes:

Taking the image challenge on the NEJM site i guessed wrong on a lung
sputum sample. Desperately trying to recall lung involvement parasite.

Ascaris? Bzzzzzzzt!

Of course not, dummy. Too quick on the mouse.

It was a bloody egg in the lung, not a juvenile organism, and I don't
think Ascaris does the disgusting stomach to lung thing?

Turned out to be paragonimus. -> Paragonimiasis

I don't recall that one in twip

Sounds interesting,

coming soon?
[pause]

Ignore last email

D'oh! I realize you guys did touch on paragonimus. TWiP 27: Trematodes
Must have some in my brain.

Anyway, here's the link.

http://www.nejm.org/image-challenge?ci=01052012

Brian writes:

Docs R & D,
At the end of your A. lumbricoides program you discussed problems related to Toxocara canis & T. cati infection in humans, namely VLM & OLM. Dick said it would be great if someone surveyed dog runs in NYC, or nearby, for Toxocara eggs. Sampling & counting eggs sounded relatively straightforward, piece of cake was mentioned a couple times. Such a survey would be an excellent topic for an enterprising high school student, with some help from a famous parasitologist or virologist, or someone they may know. Give it some thought.

I am reading Dick's book pick a while back, "The Fever" by Shah and find it fascinating. As an anthropologist, I am well acquainted with the sickle trait balanced polymorphism, but under-appreciated the impact malaria had on human history. The rapid spread of the parasite in the US hit home after reading how fast it infected areas of New England after the environment was altered by the building of mill ponds. It took only a few returning soldiers from the Civil War to infect whole towns. Incredible.

My wife took a parasitology class a few years ago and had to draw many of the specimens you have discussed. She thought Giardia trophozoites were "cute" with their little faces. After going though a Giardia infection, I'll tell you how cute they are.

As much as I love TWiP, I am happy with the longer break between episodes so I have time for the many other terrific podcasts like TWiV, TWiM, Science Friday Video (a favorite, if you haven't seen it), NYTs The Minimalist, Science Now, Nature, Archeology Channel, RadioLab, among others. So many podcasts, so little time.

And, I have to know how you selected the fun but creepy opening music and great hard-driving riffs at the close of the show. Good stuff.

Cheers,
brian

Trudy writes:

Listening to you right now on Science 360 Radio. You mentioned this post and I had to comment. As a former nurse epidemiologist who worked in County Public Health Practice, I am a strong proponent for vaccination. I worked a number of vaccine preventable disease outbreaks including rubeola (measles) which resulted in individuals becoming needlessly seriously ill. Very much enjoy TWIV and TWIP and TWIM....My mother was also a nurse who worked in public health before most vaccines were available. She saw the tragic cases of diseases which resulted in great morbidity and mortality. I could go on and on about the good vaccines do. Thank you Professor Racaniello. Kudos to you and all who assist in the productions of your wonderful podcasts. Dr. Despommier is a treat. I have so many interesting experiences with all kinds of reportable diseases and conditions including on how I became a nurse instead of a medical illustrator or possibly a physician. I sustained a needle stick pre recombinant HBV vaccine while working my way through college in a teaching hospital. I carry natural immunity to hepatitis B now. Didn't get extra credit in my micro class, however.

Best regards,

Trudy
retired from public health but never tired of pathogens and learning about them...
Naples, Florida

Ricardo writes:

Hello Vincent and Dickson.

I just came from my first jury in a monographic work where the student cited TWIP. The monograph title was "Trichinosis: from the parasite to the ecosystem".

I was very impressed with the "watermark" left by TWIP on the student, and happy that my friend (her supervisor) suggested her to listen to TWIP.

One of my colleagues from the jury commented the text looked like a romance. She appreciated the "stories". :)

Thank you both for the really great work you are doing.

Ricardo Magalhaes, Ph.D.

Associate Professor of Microbiology
****************************
Faculty of Health Sciences of Fernando Pessoa University
Portugal

Felix writes:

Hi Professors,

In episode 16 on Giardia you discuss Antonie van Leeuwenhoek and his early adventures with a microscope. Here are a couple of interesting resources which discuss him:

Firstly an article in the ASM magazine claiming that Leeuwenhoek gets too much credit for his discoveries, some of which should be shared by Robert Hooke of the Royal Society of London. http://forms.asm.org/microbe/index.asp?bid=27982

Secondly, an excellent BBC documentary "The Cell", which begins with Hooke and Leeuwenhoek. http://topdocumentaryfilms.com/cell/

You have also mentioned Paul De Kruif's book "The Microbe Hunters" as one of the few sources for information on the history of germ theory and microbiology. A recently published book (also from the ASM) addresses the same area, most likely with a less idiosyncratic style than the dated De Kruif volume:

Germ Theory: Medical Pioneers in Infectious Diseases
Robert P. Gaynes
ISBN: 978-1555815295

Just while writing this email I discovered the ASM book store with a section of the history of science http://estore.asm.org/browse/index.asp?plid=2&categoryid=20.
(I have no connection with the ASM!)

I am currently re-listening to all of your TWIP podcasts, having listened to them all at least twice when they were first released. They're are just that good!

I really love the beginner-level approachability of the first 30 or so episodes.
It would be great if you could cover a small number of highly important non-parasitic diseases, such as cholera, in the same style.

best wishes,
Felix

Jim writes:

Hi,

I'd really like to hear the details of your efforts to convert Dickson's text to electronic format for access over the web. Lessons learned should be useful to anyone with similar thoughts. How is it different from creating such a work from raw materials? I'd prefer it as part of the TWIP's, if that is acceptable.

Thanks.

I'm still hear all the TWIM, TWIP and TWIV's, but my podcast listening load is heavy (60/wk) and production of a weekly blog about the best entries is slow for an old-timer.

Loved Dickson's link to the stop-action high-speed photos of the liquid drops.

Jim
Smithfield, VA

Sven-Urban writes:

Dear Profs Racaniello and Despommier,

As a long time listener of TWiP (and for that matter TWiV and TWiM) I feel obliged as well as entitled to express some - mild - criticism that I feel and hope is appropriate.

While Your mutual friendship is a much valued aspect of the show, I think it might lead You to a degree of bantering that interferes with the contents. Slightly too often I find Your discussion interrupted by mutual jibes, which make me lose the thread of thought. I would say that generally, though not exclusively, Dr Racaniello is the instigator of these interruptions. I am, of course, not now referring to those abundant, seriously intended and equally seriously phrased questions that Dr Racaniello interjects, but rather about occasional stray comments and jibes that appear in private between very close friends. I think the term "in private" here is crucial, as I doubt that You would express Yourselves like this were the two of You jointly presenting before a class.

Today I could, by the way, compare TWiP #37 ("Dracunculiasis") with TWiV #172 ("Two can be as bad as one", with Kathy Spindler) - both in a one-on-one setting - and the difference in tone is remarkable.

Naturally I speak here only for myself, of course, but I for one would appreciate if You might henceforth tone down the sparring, keeping in mind that You do actually appear before a large, international audience, not all of whom might appreciate or be able to follow Your bantering. (And just to be precise, "tone down" does not necessarily mean "discard".)

This notwithstanding I am very grateful for the set of podcasts You so graciously provide us all with!

Sincerely and Respectfully, Yours
Sven-Urban
Sweden

Robin writes:

This from http://thefreedictionary.com:

ac·ta
[ak-tuh]
- plural noun 1. Often, Acta. official records, as of acts, deeds, proceedings, transactions, or the like

Origin:
< L, neut. pl. of āctus, ptp. of agere to do; cf. act

Robin writes;

The TWIP podcasts again emphasize to me the difference between hearing first-hand from someone with experience in contrast to hearing material regurgitated by someone with book-knowledge. It made a world of difference listening to a trauma surgeon or an emergency physician from a trauma center speak in contrast to similar material presented by someone without direct experience.

Sarah writes:

Greetings Dickson and Vincent!

I am a new listener and I am thoroughly enjoying working my way through the TWiP and TWiM podcasts. I found your conversation regarding Draculunculiasis very informative. I think it is great that you spent a good portion of time emphasizing the necessity for clean water for overall public health. It is something that we often take for granted here in the U.S. Something caught my attention though. You talked about the use of cheap cloth to filter water in the prevention of Guinea worm and mentioned that it was unfortunate that this wouldn't prevent diarrheal diseases. I attended a session at the ASM general meeting a few years ago to the contrary. While Sari cloth won't prevent viral borne diseases, it does seem to reduce the incidence of Cholera. Here's the link to the study which was published in the first issue of mBio:

http://mbio.asm.org/content/1/1/e00034-10.full

Kind Regards,

Sarah
Assistant Professor of Biology
Gordon College
Barnesville, Ga

Ian writes:

Dear Drs. Twipaniello and Twipommier,
On the topic of the Dracunculus life cycle: Is there evidence that a male is required for fertilization? Some nematodes are parthenogenic and "virgin birth" for this worm would be a nice compliment to the biblical story of the cadueceus (sp??).
Keep up the good work,
-Ian

Alicia writes:
Hello,

I`ve heard that Toxoplasma gondii might be caught by touching a leech. What`s the general consensus on this?

Thanks and keep up the great work.

TWiP 37 Letters

Cam writes:

Dick Despommier is often talking about the importance of ecology when understanding parasitism. Does he have any suggestions for good introductory texts to the subject for someone of my lowly level?

I recently came across this article, which led me to the idea of Oogst. Has Professor Despommier come across this, and how does he view it in relation to the idea of Vertical Farming?

Raihan writes:

Hello Guys,

Sorry but I wanna share a non-parasite related news story with you guys.
Came across this article about how the government in Singapore is deciding to invest in roof-top gardens here, as a measure of controlling the recent cases of flash flooding here.

When I read it, i was reminded of Dr Despommier's brain child, the Vertical Farm. I guess this is not exactly a farm but it has a lot in common and displays another way of how incorporating fauna in cities can help solve problems in an urban setting.

http://www.eco-business.com/features/cheap-and-quick-green-roofs/#comment-2021

You guys may choose to read this email on your Vertical Farming Podcast instead of TWIP.

That should be coming pretty soon right? *cheeky grin.

Love the show

Matt writes:

Dear Dickson and Vincent,

(Not sure if I sent this to the right place on the microbe world website, but as I noticed that you gave this email address at the end of the podcasts I thought I'd try it as well...)

Thanks for your wonderful series of podcasts; I have been working my way through all of TWIP and you are currently keeping me sane whilst I data-enter several thousand clinical records from a malaria prevalence survey. I also have TWIV on CDs which I listen to in my car on my commute route to my workplace in Muheza, North East Tanzania. And I'm very excited by TWIM which I will indulge in soon.

I am a medical entomologist currently working on insecticide-treated materials against malaria vectors and have a question which I hope you'll discuss on TWIP in relation to malaria but also in a broader context. It is this: do you think we already have enough tools to control (many) infectious diseases and that funding should be channeled into improved application/delivery of those tools and away from the search for novel techniques and 'blue sky' research? For malaria, we already have a diverse arsenal of proven weapons and auxiliary tools including bed nets, indoor residual spraying, intermittent preventative treatment, house screening, rapid diagnostic tests; GPS and mobile phone systems have vastly improved surveillance, we have subsidized and highly effective frontline drugs for treatment, amazing repellents and lures for mosquitoes etc. etc. People like Fred Soper and Malcolm Watson were incredibly successful at reducing malaria 60+ years ago with a much more limited arsenal. You might say that there was much less resistance to insecticides and anti-malarial drugs in those days, but in recent times two brilliant weapons emerged that are still being used, to which there was no resistance, i.e. pyrethroid insecticides and artemisin-based combination therapy (ACTs). What do you think are the major stumbling blocks in infectious disease control? Is the health sector too slow to act on research findings*?

Many thanks and keep up the good work.

Matt, National Institute for Medical Research, Tanzania & London School of Hygiene and Tropical Medicine

p.s. I wrote this before I saw the topic of Twip 35! I hope this question is still relevant (and I'm going to listen to that episode now once I can download it!)

p.p.s. I thought you might like to know that my two dogs are named after you! (Vincent and Dickson - jpg)

Dr Matthew J Kirby
Fieldsite Project Manager PRISM
Amani Research Centre
National Institute for Medical Research
Muheza
Tanzania

Heather writes:

Dear Doctors R&D,

I love your podcast! It is wonderful to listen to commuting to work and while I am working as the support technician for the chemistry and microbiology teaching labs at our small state college. My favorite thing about your show is that the format is similar to the "seminar" or "journal club" courses that were my favorite in graduate school (that was back when I too wanted to be a "dr.", before I became a lapsed microscope jockey. Maybe I will go back to grad school....maybe...some day). Basically these 1-2 credit courses consisted of grad students getting together with a professor to pick apart papers in specific disciplines such as microbiology (we went over the "old" "elegant" research that was the foundation of modern microbiology), microbial genetics (we called that one "cloning club), current topics in molecular biology, current topics in elasmobranch biology, and current topics in shellfish aquaculture. My background is in fish and shellfish pathology, specifically the microbial and parasitic (and molecular aspects thereof) diseases of cultured fish and shellfish. Bearing that in mind, while I realize that you focus your podcasts on human/public health issues related to parasitism, might you consider doing an episode about epizootic parasites (or microbes or viruses) that impact humans economically and/or ecologically? Some examples that come to mind are my good friends QPX (quahog parasite unknown) or Perkinsus marinus in the New England shellfishery, "bumper car" disease in Long Island Sound lobsters, or the recent controversy surrounding the ISAV (infectious salmon anemia virus) outbreak in the Pacific Northwest. Then of course there are always parasites that are just fun for their "gross" factor like "salmon poisoning disease". Regardless if your decision regarding the discussion of parasitism of non-human animals I will continue to look forward to your TwiP, TwiV, and TwiM podcasts.

Thank you!

Heather

P.S. I read "Parasite Rex" as a freshman in college and it is still one of my favorite books!

P.P.S. "Monsters Inside Me" sometimes grosses me out, and that is saying a lot for it's accuracy!

TWiP 36 Letters

Trudy writes:

Dear Drs. Despommier and Racaniello,

I am almost caught up listening to TWiP! I look forward to your future efforts and eBooks, etc., however, in TWiP # 32, I thought I heard you mention that there would be a link to Dr. Despommier's lectures. I couldn't wait to go to your website and check them out. Unfortunately, the link was missing. You know what they say about the missing link, don't you? It must be a cryptid! But seriously, hope you will post the link to the lectures at your next opportunity. Please? :-)

I am dreading listening to # 33 because there are no more TWiPs available yet. I live in a semi tropical climate in SW Florida so tropical diseases and parasites are especially interesting. The only good thing about being current with your series, is that I can start to catch up on TWiVs and TWiMs. I did find Dr. Racaniello's lectures in Virology on iTunes and am watching this series. I started with the hepatitis Delta virus. It is of special interest to me as are all forms of viral hepatitis.

Dr. Despommier, please consider doing a podcast on vertical farming and even throw in some other tidbits of gardening wisdom.

I took the user survey as you request. Perhaps the results will help you in your future efforts. The way you two work together on TWiP makes for a delightful experience. Science, medicine and pathogens "float my boat" so it is a great pleasure to have two learned professors so engaged in interesting discussions.

I have written before. Hope this isn't too much. Consider that you are talking (via my earbuds) straight into my noggin: I feel like I know you both and it is an honor.

Best regards,


Peter writes:

http://www.livescience.com/13040-10-disgusting-parasites-zombie-ants-toxoplasma.html

Brian writes:

Vincent & Dickson,

My wife pointed me to TWIP and what a blast. My 23 year old son and I are making our way through the early episodes during our commute to and from work and loving every minute of it. After listening to the tapeworm episodes I will NEVER pet a dog from Wyoming or any other of the sheep states.

I'm not sure if you have covered the evolution of parasites, we're only up to episode 9, but that must be as crazy as ever. Surely you have enough material for several years worth of podcasts, but I'd like to hear a bit about how an animal accumulates such a bizarre life history.

Cheers,

Peter writes:

Dear TWiP team.

I was speculating about parasites that modify host behaviour and a possible strategy that may have evolved in those transmitted by blood feeding vectors.

To achieve optimum transmission of the parasite uninfected blood feeding insect vectors should find infected hosts more attractive than uninfected ones and once the insect vector had itself become infected its behaviour should change so that it would then preferentially seek uninfected hosts.

Has any research been done to determine whether any parasites that employ blood feeding vectors actually do modify host smell and vector preference in this manner?

As I was writing I was thinking of arthropod vectors such as mosquitoes and biting flies but it occurred to me that there are other possible blood feeding vectors such as vampire bats and leaches whose behaviour could also be potentially modified in this way.
Are there any zoonotic infections that are transmitted by leach vectors?

Lastly, I have seen blood feeders described as Hematophagous and Sanguivorous are these synonymous, and if not what is the correct/preferred term?


Peter
Turkey

Gopal writes:

Hello, Vincent & Dick,

This month's issue of The Scientist has this interesting article:

http://the-scientist.com/2012/01/01/animal-mind-control/

Can't help wondering, somewhat uneasily, what our own parasites make us do!

Best,

Gopal


Don writes:

In your discussion of Wolbachia in filariasis, Vincent suggested tetracycline for therapy. Dickson opined that it was too toxic and expensive . Au contraire, in the QID form it is "dirt cheap" and has been used widely since the mid 50's. Prolonged use does cause mottling of the teeth, and florescence of the bones under Woods light, not a problem for most of us. Wonderful Podcast, and deeply appreciated. Thanks

Kyle writes:

Top O' The Mornin' To Ya! (I'm not Irish - I just like the phrase..)

I would like to thank you for putting together such an excellent podcast. I'm currently a Med Tech working in a parasitology laboratory and enjoy my days searching through blood and pooh for those tiny life forms that you describe so well. I was introduced to the world of parasites by a course I took as an undergrad. The course instructor was one Dr. James B. Jensen. Judging from your earlier talks, I trust that this name will sound familiar. As you can imagine, the course taught by such a well known parasitologist was an amazing experience. I have a great passion for parasitology, and I'll see what kind of future I can put together. Thanks again for the great shows. I encourage you to continue, and add my voice to those that are calling for more. It is This WEEK in parasitism, you know! :)

-Kyle

Jim writes:

Vincent and Dick,

Delighted to hear the latest TWIP on the malaria vaccine. You guys mentioned using impregnated bed nets to reduce malaria. The NY Times had an article at: http://www.nytimes.com/2011/09/27/health/27mosquito.html

This article described using poisoned bait to reduce the mosquito populations by a bunch like 90%. Would love to hear your thoughts on this.

Was also delighted to hear you guys are going to have more TWIP.

Am still devoted to TWIP, M, V.
Thanks much and 73

Kelly writes:

Hi, Vincent and Dickson.

I'm a PhD student at the University of California Davis and a long-time listener of This Week in Parasitism and This Week in Virology. I have a great appreciation for the fact that you two are successful scientists who take time out of your busy schedules to share your love of parasites and viruses with the general public. In fact, I was so inspired by This Week in Parasitism that I have since begun podcasting about science topics myself! Thanks for the inspiration!

I thought you two might be interested in an "experiment" in science crowdfunding called the SciFund Challenge. One exciting aspect of this experiment is that we're exploring a potential new source of funding (i.e., the general public) for small science projects at a time when everyone seems to be complaining about how difficult it is to get grant money. While this aspect of the experiment is certainly exciting, the most exciting part to me is the fact that crowdfunding forces scientists to engage with the public. Not only does the public get a chance to learn about cutting edge research, but they get the opportunity to have a voice in deciding the direction of future research through the money they donate.

While I think you might be interested in learning about a program incentivizing scientist engagement with the public, my motives are not entirely altruistic. I have a SciFund project focusing on how trematode parasites manipulate the behavior of their hosts, and I'm wondering if you would be willing to help me get exposure to my RocketHub proposal for this project (URL is here: http://www.rockethub.com/projects/3737-support-zombie-research, or go to RocketHub.com and search "Support Zombie Research"). I study Euhaplorchis californiensis (EUHA), a trematode parasite infecting the brains of California killifish. EUHA manipulates the neurochemistry and behavior of their fish hosts, a fact which I find endlessly fascinating. These parasites have essentially had millions of years to figure out how the fish's brain works, and to figure out how to manipulate brain chemistry to achieve their own goals. I'm working on quantifying the extent to which the parasites modify the behavior of their host, and am trying to figure out the mechanism by which these parasites achieve their extraordinary behavioral manipulation. I believe parasites have a lot to tell us about how brain chemistry influences behavior, and I think of my research as an exercise in coercing EUHA to tell me what it has learned over millions of years about brains and behavior.

If you would be willing to tweet about my project or mention it in any other way, I would be endlessly appreciative. I'm happy to answer any questions you might have about my study system, as it's pretty much my favorite thing in the world to discuss!

Trudy writes:

Dear Drs. Racaniello and Despommier,

I just discovered your wonderful Podcasts! I am a registered nurse who has an unusual background. I worked as a diener at a teaching hospital while working my way through college. My mom was an RN also when there were few vaccines. She graduated in 1935. She would often discuss what she had seen as a public health and Army nurse anesthestist in WWII. She had a library of medical textbooks that I enjoyed reading . I spent part of my career as a nurse epidemiologist (county public health practice) and as an STD clinician, where I got to work with all kinds of infectious diseases and conditions (viral, bacterial and of course parasitic.)

I have been interested in infectious diseases and conditions since I was a young child when I got my first microscope. Also I have been lucky enough to live in semi tropical Florida for over 30 years. The University of S. Florida still has a great series of podcasts from their medical school's Division of Infectious Diseases. They also discuss tropical medicine and have a College of Public Health.

My first in person experience with a parasite specimen which really "hooked" me on microbiology was that of a tapeworm which was in a huge (tall) container in the foyer of Abbott Labs in Chicago. I was with a group of High School students which had a NSF grant in collaboration with Northwestern University's College of Medicine.

I have been fortunate to see a lot of interesting things because I also did some bio-medical illustrations. I am both an artist and a nurse.

To make a long winded email shorter...Your podcasts are infectious...I believe there is no cure or treatment other than continuing as a listener to you discuss microbes in your most engaging fashion.

Thank you for sharing your knowledge and experiences!

Trudy in Naples, Florida

Paul writes:

Hi guys

Just discovered the podcast.
Listening to 2 shows a day ...working my way up.....absolutely love it....love dr. House ..parasite rex....and monsters inside me:)
While gathering information about parasites i often see these so called zappers little devices that kill parasites ..what are your thoughts on these.....and the history of using electric devices in killing parasites.

Greetings from switzerland

Josh writes:

Dear Drs. Despommier and Racaniello,

I am an auditor. To occupy my mind while I work I started scouring iTunes for science rich content. I saw your show "TWIP" and thought why not? I think the first TWIP I listened to was #33. I was immediately hooked. I went home and subscribed to TWIP, TWIM, and TWIV that night.

Currently I am listening to all of the TWIPs in a row before I start the other shows. I love the education, the ick factor is pretty high but this show provides me with an EXCELLENT free education, and helps me confront fears that were boogey men in my mind by giving me real quantifiable behaviors and characteristics of many things I have had uninformed fears about ( i.e. Trichinella, Toxoplasmosis, and tapeworms).

I am very saddened that I didn't know who Dr. Despommier was prior to November of this year. In the Spring of 2011 I was writing a business plan for my entrepreneurship class and I advocated that my group do our project on vertical farming. We were unable to find any resource that would provide us with information on how to successfully integrate a vertical farming business into an urban area such as Phoenix AZ, where our fictitious business had to be. We had thought about potentially buying a tall structure like an empty condo tower, or office building and converting it into a farm, or placing farms on roof tops (kind of like they do in Cuba). Sadly we couldn't find any cost to benefit analysis, yield per acre data, or potential profit margins with which to build our fictitious business plan. We ended up using another idea of mine, recycled building material sales. I remain sad that we were unable to write a plan for vertical farming.

Thank you for providing me with such a wealth of free information. Thank you for presenting it in such a content rich way, without spoon feeding the listener. Please continue to do these shows. I am indifferent to frequency as long as I can continue to have access to your knowledge and experiences.

Thanks,

Rachel writes:

Dear Dr. Racaniello and Dr. Despommier,

I'm in the process of choosing a career (PhD or MD)

I would love to follow in your footsteps and become a professor, researching parasites and leading the young minds to new discoveries! However, when talking with my parasitology teacher it seemed like he was discouraging me due to the lack funding and interest in the subject. I wanted to know your opinion, especially Dr. Despommier's. Do you think part of your success in your area of research came from the 'newness' of the subject or is there still an opening for hopeful parasitologists.

Thanks for all the work you both put in to the TWI series, I am a huge fan.

I am waiting anxiously for the next installment of TWIP.

TWiP 35 Letters

Trudy writes:

The more I listen to your fantastic podcasts, the more I love them. wO0t. I am the Naples, Fl nurse/artist who has written before.

Because I did get the pleasure of tracking reportable diseases and conditions in Florida, I was struck to know that other states did not require reporting of giardiasis.
Thought you might find the link to the list in my state interesting.

http://www.doh.state.fl.us/disease_ctrl/epi/surv/reportable_diseases_08.pdf

I do have a question...if you answered it can you mention the podcast number, please? It is more of a TWIM question, but I would like both of your comments because of the urban gardening interest by Dr. D. When tomatoes were suspected of being the source of a widespread salmonella outbreak in the not too distant past, there was speculation that the organism was contained within the tomatoes' cells!!! I garden, was a biology art major before finishing my BSN. This made absolutely no sense considering the differences between animal and plant cell structure. Also, what does Dr. D. think of organic gardening?

You also may find it interesting that Florida does allow land application of bio solids after processing in a wastewater treatment plant. Considering some of the encysted pathogens, I always felt this was unwise. Not every County allows it.

All the best from your very loyal listener...life long learner with ear buds which are rapidly embedding as a result of your "infectious podcasts"...

Trudy

Dang, you are the best, guys. Listening to the podcast about crypto right now.

Gave you 5 stars and wrote a "glowing review" (ala Mark Crislip) on iTunes.

Alexander writes:

Dear Drs. Despommier and Racaniello,

I've really enjoyed listening to your podcast and learning about all of the fascinating parasitic diseases that they don't teach us enough about in medical school. I'm a student at Boston University School of Medicine but currently taking a year off to do HIV research in Uganda. Among other things, I'm hoping to perform a retrospective study looking at the impact (if any) of soil-transmitted helminth infection on antiretroviral treatment outcomes in a cohort of HIV-infected individuals in rural Uganda.

At any rate, my real question for you has to do with treatment of schistosomiasis. I happened to be swimming in Lake Victoria last weekend (self-preservation is not one of my strong points...) and am thinking it may not be a bad idea to take some PZQ. My question is: how long does one need to wait after potential exposure to schisto for PZQ to be effective? My favorite new textbook, Parasitic Diseases 4th Edition by Despommier et al (have you heard of it?) says that PZQ is effective against the adult organism. However, it sounds like it takes several days for schistosomulae to migrate from the infection site to the liver, only then at which time they develop into adults. Provided that schistosomulae are also susceptible to PZQ and the drug has good subcutaneous tissue penetration, it should be fine to prophylax even within the first days after exposure. However, if this is not the case it may be necessary to wait some period of time to ensure that any schistosomulae have had time to transform into adult worms before administering PZQ. Any thoughts on this?

Finally, I just wanted to say thanks for taking the time to create and sustain TWIP. It's always a pleasure to listen in, and I'm actually finding the information you provide in the podcast (coupled with your wonderful textbook) to be quite clinically relevant here in Uganda.

Thanks, and all the best,
-Alex

PS - here's an idea for a future TWIP episode: a review of clinically relevant (or otherwise interesting) interactions between parasitic infections and other infections, e.g. genital schisto increases susceptibility to HIV infection in women. I think one important and possibly under-emphasized point by Peter Hotez and others at the forefront of advocacy around NTDs is that it is valuable to target NTDs not only for the purpose of eliminating these infections themselves but also for mitigating any pathogenic synergy they might have with other more "sexy" diseases like HIV.

Cedric writes:

Dear Vincent and Dickson,

I recently read this paper discussing the phenomenon of self medication in animals. The authors speculate that this behavior was selected for, at least in part, due to the antiparasite properties of many of the plants, animals, or materials consumed by animals for reasons other than nutrition. I was wondering if Dickson had any more information on this process, and what his opinions are regarding the origin of self medication in regard to parasites.

Thanks so much for all of your work in developing TWiV, TWiP, and TWiM. Your discussions have given me a real head start in my undergraduate biology classes, and I look forward to every episode.

Cedric

Ricardo writes:

Novel Drug Wipes out Deadliest Malaria Parasite Through Starvation

http://www.sciencedaily.com/releases/2011/12/111207152416.htm

Theodore writes:

Good day (or night),

Starting from October I've listened to about half of the TWIVs, all the TWIMs and the TWIPs. I find them to be very enjoyable, the semi-Socratic method of teaching is quite refreshing. However, two particular subjects have been underplayed.

First Toxoplasma. The papers by Fekadu, Lindova and McAllister are great primers for the studies regarding the behavioural effects of T. gondii. The Gaskell one is by far the most interesting though. So, hands down, the leading theory for schizophrenia's etiology is an inappropriate level of L-dopa, the precursor for such molecules as dopamine and epinephrine. Depending on the population studied, seroprevalence of T. gondii induced IgGs among patients with schizophrenia is as high as 97.7%. Though there is some ambiguity as certain studies report a 42% seroprevalence amongst schizophrenics, compared to 11% in controls. Regardless of the exact rate, it has a statistically significant presence in schizophrenics and people with other mental health problems.

Just a correlation though. A tongue in cheek refute of correlative studies is the stork theory (will be paraphrased/butchered). The idea is that from 1970-1985 there was a surge in the German birth rate. At the same time, there was a surge in the stork population of Germany. Their coincidence was statistically significant. Of course, we know this is bupkis. Storks and babies aren't actually related. However, if we found that there was a drop in condom sales, we could have an aha moment, due primarily to the fact that we can name the mechanism for why that correlation actually matters.

Back to Gaskell, and the aha mechanism for toxo and schizophrenia. Their group shows that T. gondii has two fully functional enzymes specific for producing L-dopa, and they show that these genes are transcribed specifically when the parasite infects neural tissues, and that the enzymes produced do make L-dopa. We really can't prove that toxo causes schizophrenia with Koch's postulate since putting a brain parasite into a volunteer isn't all that nice. However, I'm flummoxed to think of anything else that is needed to point at T. gondii as the causative agent for some types of schizophrenia.

Ricardo writes:

Global warming wilts malaria

http://www.nature.com/news/global-warming-wilts-malaria-1.9695

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