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Martinus Beijerinck uses enrichment culture, minus nitrogenous compounds, to obtain a pure culture of the root nodule bacterium Rhizobium, demonstrating that enrichment culture creates the conditions for optimal growth of a desired bacterium.

More Information:

Early Biotechnology: The Delft Connection, ASM News 59, 1993. p.401 [pdf]

Beijerinck, M. 1888. Die Bakterien de Papilionaceenknollchen. Botanische Zeitung, Vol. 46: 725-804. In Milestones in Microbiology: 1556 to 1940, translated and edited by Thomas D. Brock, ASM Press. 1998, p220 [pdf]