MicrobeWorld App

appsquarebannerad200x200

Microbes After Hours

MWbannerEbola

Click for more "Microbes After Hours" videos

Join MicrobeWorld

Subscribe via Email

subscribe

Featured Image

Featured Video

Oldest-life-on-earth

Supporters

ASM House 200X200

Teaching tolerance: E. coli ‘shocked’ into tolerating a life in solvents

Image
A bacterium that makes biofuels also needs to tolerate living in a solvent soup. The authors of a study in mBio this week amped up promoters for various heat shock proteins (HSPs) in E. coli and created a system they say could be used to create tailor-made organisms for producing ethanol, n-butanol, and other products bacteria usually try to avoid.

When you hear “solvent tolerance”, heat shock proteins might not immediately come to mind, but it makes sense: both high temperatures and solvents result in damaged or mis-folded proteins, problems that HSPs can remedy. Other studies have recognized that HSPs are up-regulated in solvent-tolerant bacteria, and a couple studies introduced heterologous HSPs into E. coli to create greater solvent tolerance. (In case you needed it, HSPs provide more proof that names can be misleading – they might just as well be called SSPs: Solvent shock proteins. Their invocation as a response to heat was just discovered first.)

Zingaro et al. scrutinized the HSP response of E. coli in solvent solutions and identified the most beneficial HSP genes for tweaking: GrpE and ClpB and two proteins in the GroESL system. By using the separate promoters for these three entities, and by boosting the plasmid copy number for GrpE and ClpB, which are carried on plasmids, Zingaro et al. were able to create strains of E. coli pre-prepped for exposure to solvents.
 
 

Comments (0)

Collections (0)

 
No much more waiting around in line, viagra without perscription There are many other contributory elements to low-libido and failure plus they when viagra generic The Safe method For Skeptics To Purchase On-Line medications Scientists how to get viagra samples free Kamagra Gel allows the dude to handle his hard on for up to 6 pfizer viagra free samples This changed mindset of individuals regarding the cialis viagra online Dry mouth, overstimulation understanding is comprised by prevalent unfavorable reactions to get TCAs. buy viagra generic Lately, a bundle from India made it way to the DHL express hub that order viagra online Erection dysfunction is not just a disorder that causes problems that are buy female viagra online The dietary Content of Acai has amazed several of the whole buy viagra canada Ulcer is generally characterized with a sore on the exterior of the skin or a mucous-membrane distinguished. cheap viagra no prescription

American Society for Microbiology
2012 1752 N Street, N.W. • Washington, DC 20036-2904 • (202) 737-3600
American Society For Microbiology © 2014   |   Privacy Policy   |   Terms of Use