Readers of virology blog often request explanations of specific experimental techniques. Methods such as complement fixation, deep sequencing, ELISA, PCR and many others are frequently mentioned on this blog without discussion. To do so would interrupt the scientific discourse and make for lengthly posts. To remedy this shortcoming, I have added a new tab to the first page of virology blog called Virology Toolbox. This page will be populated with explanations of experimental techniques used for the study of viruses. Today’s technique is the western blot.