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Significance of Controls and Standard Curves in PCR

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Are you looking for help and more explanations about the proper controls for PCR and how to prepare standard curves? If you are, then you may want to attend this online seminar by expert, Ian Kavanagh, from Thermo Scientific R&D. He is going to give a 30 minute presentation on the subject Tuesday June 2nd at 9 a.m. Pacific/ noon Eastern.

To sign up for a reminder, and to see the upcoming seminars, go to the source link.

Whilst qPCR is a powerful technique, the results achieved using this method is valid only if the appropriate controls have been included in the experiment. Careful selection of controls and proper optimisation of qPCR conditions promise generation of highly specific, repeatable, reproducible and sensitive data.

This presentation discusses the strategies for preparing both negative and positive controls for PCR, when they should be employed and how to interpret the information they provide. It also highlights the significance of standard curves for determining the initial starting amount of the target template and for assessing assay efficiency, precision, sensitivity, and dynamic range. It also provides guidance on how to prepare standards, interpret standard curve and troubleshoot inefficient qPCR reactions.
 
 

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