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Quick reference: Determining DNA Concentration and Purity

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The most comprehensive way to evaluate DNA concentration and purity is to use both UV spectrophotometeric measurements and agarose gel eletrophoresis. This quick reference guide gives an overview of the information that can be derived from both.

This article is a clear and concise guide to the common contaminants in DNA and what absorbance at 260, 230, 280, and 320 can tell you about the sample.
 
 

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