Problems achieving high yield and purity DNA are exaggerated in environmental samples because of the added complexity of microorganism lysis and inhibitor removal. Quantifying the nucleic acids in these samples is the easy part. But if you don’t know what to look for, you can easily make mistakes in interpreting the results, which can lead to a lot of repeat work or missed critical information in your experiments.
In this article, we discuss some of the common misconceptions surrounding DNA isolation and quanification and what problems to look out for before going to the next step with your sample.