Culturing microbes on nutrient plate and testing for different kind of microbes on specific environment areas. This inoculated nutrient plate that was left for a period of 14 days showed the present of a few different kind of fungus species. It’s common to think that the human palm contains more microbes than any other environments due to the susceptible tangibility of human hands to everything. However, this plate with sample from toilet seat showed the contradiction between human palm and the toilet seat contamination. Based on our result, the nutrient plate with a human palm sample showed no fungus presented at all compared to toilet seat nutrient plate with five different kinds of fungus. Observation: whole-colony appearance – circular, irregular, filamentous, and rhizoid; Margin – entire, filamentous, and undulate; Elevation – flat, raised, and umbonate; Diameter: 6.00cm – 1.00cm with height between 1.00cm – 0.05cm.