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952dnaAlfred Hershey and Martha Chase suggest that only DNA is needed for viral replication. Using radioactive isotopes 35S to track protein and 32P to track DNA, they show that progeny T2 bacteriophage isolated from lysed bacterial cells have the labeled nucleic acid. Further, most of the labeled protein doesn’t enter the cells but remains attached to the bacterial cell membrane.

More Information:

The Hershey-Chase Experiments

Hershey, A. D. and M. Chase. 1952. Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. Gen. Physiol. 36: 39-56. In Microbiology: A Centenary Perspective, edited by Wolfgang K. Joklik, ASM Press. 1999, p. 474 [pdf]

 

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