Developing tricks and tools to keep their enzymes in order is one way thermophiles survive. They also use techniques to keep their DNA from falling apart under intense heat. Like proteins, the parts of the long, spiral ladder-shaped DNA molecule start to unravel and break apart under high heat. One way thermophiles keep that from happening is with a helper enzyme called "reverse DNA gyrase" <jeye-race>. This enzyme makes DNA coil up and twist upon itself in a certain way that makes the DNA more stable in high heat. (If you’ve ever seen a telephone handset cord that gets twisted and bunched up on itself, then you know what DNA coiling is like, only coiled up DNA is thousands of times more tightly twisted and bunched.) Microbes that live at normal temperatures have regular "DNA gyrase" instead, which also makes their DNA coil up, but in a different, looser way.
Thermophiles also have lots of DNA binding proteins. These binding proteins do just what their name suggests, running around gluing the pieces and parts of the DNA molecule back together when they start to come undone, much like the chaperonin proteins discussed above.
Of course, tools that keep the enzymes, DNA and other inside parts of the cell from breaking up will do no good if the outside or surface of the cell is falling apart. So these heat-loving creatures have cell membranes—the rubbery lining just inside the cell wall that surrounds the cell fluid—that are formed differently than those of microbes living at normal temperatures. Normal temperature microbes have membranes that are formed by two layers of molecules called lipids which join together to create what’s called a bilayer (for a picture of what a lipid bilayer looks like, see this page). In thermophiles, the parts of each lipid layer that point inward are chemically glued together so that instead of a bilayer that could be pulled apart in intense heat, thermophiles have a thick single or monolayer.
There’s a lot that scientists still are learning about how these amazing heat-loving microbes live happily at such high temperatures. They may well have other tools and tricks that we don’t know about yet. The more we learn, the better for us because some of these techniques and tools could become useful products for us. For example, an extremozyme called Taq <tack> from one thermophilic bacterium is what makes DNA testing and DNA fingerprinting possible. Thanks in part to Taq, scientists have been able to sequence the entire human genome—everything on the whole humongous human DNA molecule—and the genomes of lots of microbes and other living things, too.