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Mycobacterium smegmatis

Streak plate of Mycobacterium smegmatis grown on TSA for 96 hrs. Read More

Streptococcus mitis on blood agar

Growth of Streptococcus mitis on blood agar demonstrating alpha hemolysis seen as a greenish color around the growing colonies due to a reduction of the hemoglobin to methemoglobin in the surrounding agar. Image taken using transmitted light. Read More

Starch Agar

Starch agar contains high molecular weight starch molecules (soluable starch). When Iodine is added it reacts with starch to produces a dark brown/purple color. A zone of clearing forms around organisms that produce the exoenzyme amylase after addition of iodine. This plate was incubated for ... Read More

BacterioFiles 196 - Flagellin Facilitates Flu-shot Function

This episode: Bacteria are important for a good immune response to unadjuvanted influenza vaccines!


(14.6 MB, 16 minutes)


Show notes: 
News... Read More

Aspergillus clavatus

Picture carefully taken with my iphone under a dissecting microscope. Read More

Mannitol Salt Agar (MSA)

Mannitol Salt Agar (MSA)
•Important Ingredients: Phenol red, mannitol (sugar), 7.5% NaCl
•Differential: between pathogenic S. aureus and S. epidermidis
•Selective: NaCl inhibits non-halophilic organisms. Organism that can grow are halophilic.
•Mannitol Fermentors: fermentation of acid cau... Read More

ASM GM 2014 - Where Pathogens Can Linger on Airplanes

Many air travelers are concerned about the risks of catching a disease from other passengers given the long time spent in crowded air cabins. New research shows disease-causing bacteria can linger on surfaces commonly found in airplane cabins for days, even up to a week. Participants discuss ... Read More

Tube morphology

Three organisms inoculated in TSB to look at tube morphology. From left to right:
Staphylococcus aureus: flocculent growth/turbid, growth throughout the tube.
Mycobacterium smegmatis: pellicle, growth at the top of the tube. M. smegmatis tends to stick to the tube and grow up the side.
Ba... Read More

Shoe Swab

Unknown organism isolated from a swab taken off the bottom of a shoe. Students were asked to swab something in the classroom. One swab was done on the bottom of a shoe and incubated on a TSA plate for 48 hrs at 37 degree’s C. The organism was difficult to scrape so a small chunk of the organis... Read More

E. coli gram stain

Gram stain of E. coli showing characteristic G- rods. Read More

Beauty of Nocardia asteroides Colonies on Blood Agar

Nocardia asteroides isolation from blood specimen. On culture, chalky white colonies were seen on blood agar. Image courtesy MicrobeWorld user Kyriakos Zaragkoulias, Specialty Registrar (StR) in Medical Microbiology at General Hospital of Thessaloniki “G. Papanikolaou”, Greece.


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Salmonella grown on Salmonella-Shigella (S-S) Agar

The following picture shows the isolated black colonies of Salmonella on Salmonella-Shigella (S-S) Agar. S-S Agar is used as a selective and differential medium for the isolation of Salmonella and some Shigella species. The inclusion of bile salts is to inhibit gram-positive organisms. Ferric ci... Read More

Bacitracin test on Streptococcus pyogenes

Bacitracin test done on a lawn of Streptococcus pyogenes grown on blood agar. The zone of inhibition around the bacitracin disc, approx 14mm measuring the entire length of the zone, indicates sensitivity. The zone of inhibition is red because the red blood cells did not lyse. Grown for 24 hr... Read More

Methyl Red/Vogoes-Proskauer (MR-VP)

Methyl Red/Vogoes-Proskauer (MR-VP)
One medium used for two separate but related tests, identifying butanediol (VP) or acidic fermentation products (MR) as part of the IMViC test used in the identification of Enterobacteriaceae.

•Methyl Red (MR): detects the ability of an organism to produc... Read More

Unknown environmental Contaminant on blood agar plate streaked with S. pyogenes

A streak plate of Strep pyogenes on blood agar, stock culture used for class, was kept at room temp for 1 month. Before disposal a contaminant, presumably environmental, was seen on the plate. The pigment was opaque tan/yellow and with a mucoid surface. Lobate marigins with raised irregular-rh... Read More

Bacillus subtilis

Streak plate isolation showing colonial morphology of Bacillus subtilis on trypticase soy agar (TSA) incubated for 24 hours at 37oC. Note the rough and dry/matte texture characteristic of this organism’s growth. Read More

Bacillus subtilis

Gram stain of B. subtilis showing characteristic G +streptobacillus morphology. Read More

Contaminant snowman

Some holiday fun from last winter composed of different collected plate contaminants. Based on colony morphology and wet mounts, these bacteria are likely to be: Staphylococcus sp. (white), Serratia marcescens (dark orange), and Micrococcus luteus (creamy yellow). The design was incubated for 5-... Read More

Graphic Warnings: Ebola Posters Keep The Virus On People's Minds

The campaign is called "Kick Back Ebola." But the posters pack a punch.

Sierra Leone has reported over 700 suspected Ebola cases, more than any other country this year. To help stop the outbreak, health workers have put up Ebola awareness signs all over Sierra Leone's seaside capital of Freet... Read More

Kirby-Bauer disk diffusion

Kirby-Bauer disk diffusion susceptibility test on coagulase-negative Staphylococcus aureus and E.coli grown on Mueller-Hinton agar with tetracycline (30 µg), cephalothin (30 µg), erythromycin (15 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), streptomycin (10 µg), and novo... Read More
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