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INDIA INK Preparation for Cryptococcus .

india ink preparation for cryptococcus neoformans.

this organisism is isolated from suspected of cryptococcosis from HIV infected patient, 45yrs/M.this work is performed at , deparment of Mycology at TU, Teaching Hospital, Ktm, nepal.
Phenotypic test done for conformation of this organisms... Read More

Have a Happy Bioluminescent Fourth of July!

In this post from my Microbiology/Education blog, I wish everyone a lovely day of food, fellowship, and fireworks. My laboratory brings you our own "fireworks" through bioluminescence! Read More

Bacillus subtilis

Gram stain of B. subtilis showing characteristic G +streptobacillus morphology. Read More

ASM program in "Scientific writing and publishing"

Dear Board,
Here is the one JPG picture attachment. please find it out here.In 20 April 2014 here in Nepal, we have ASM program in "Scientific Writing and Publishing" by ASM young ambassador to Nepal Dr.shyam prakash Dumre. We can see in below picture students posing for group photo and are in... Read More

Conidiophores in Neurospora crassa

This is an 3D image of conidiophores of the filamentous fungus Neurospora crassa. Conidiophores are hyphae which differentiate to produce asexual structures named spores or conidia. The image taken using a confocal microscope shows conidiophores stained with a fluorophore which stains components... Read More

Microphotograph showing scolex of Echinococuus granulosus.

Microphotograph showing scolex and hooklets of Echinococcus granulosus. Read More

Macroconidia of Alternaria alternata

I have taken this microphotograph of macroconidia of Alternaria alternata.

Sheikh Imtiyaz
Department of microbiology
SKIMS Soura Srinagar India Read More

Thesis work on mycology for the degree of PG in Medical Microbiology.

My name is Sangeeta Khadka and i am from Nepal. I am a PG level student at St. Xavier's College. Recently, i have been doing my thesis work on mycology in Nobel Hospital and Research Centre for the degree of PG in Medical Microbiology.

This is a picture of different Candida species isolated ... Read More

microbes style

A microorganism (from the Greek: μικρός, mikros, "small" and ὀργανισμός, organismós, "organism") is a microscopic organism, which may be a single cell or multicellular organism. The study of microorganisms is called microbiology, a subject that began with Antonie van Leeuwenhoek's discovery of m... Read More

microbes to microorganisms

Microorganisms are very diverse and include all the bacteria and archaea and almost all the protozoa. They also include some members of the fungi, algae, and animals such as rotifers. Many macro animals and plants have juvenile stages which are also microorganisms. Some microbiologists also clas... Read More

Streptococcus pyogenes Gram Stain

Gram stain done on S. pyogenes showing the characteristic long G+ streptococcus chains. Culture grown on blood agar for 48 hrs at 37 degree’s C. Read More

Mycobacterium smegmatis Acid Fast Stain

Acid Fast stain done on a mix of Staphylococcus aureus and Mycobacterium smegmatis. Carbol fuchsin was applied to the smear and set over a steaming water bath for 10 min to help penetrate the mycolic acid in the cell wall, then rinsed with acid alcohol which washes the carbol fuchsin out of al... Read More

Slaying bacteria with their own weapons

A chemist at Washington University in St. Louis is studying siderophores, iron chelating molecules released by bacteria during an infection, with the thought of using them to design personalized antibiotic therapy that would avoid the rapid evolution of resistance that plagues antibiotic drug di... Read More

Endospore Stain Bacillus cereus

B. cereus was grown on urea agar for 6 days at 37 degrees C to induce spore formation.
The endospore stain was done using malachite green as the primary stain and safranin as counter the stain. Malachite green was applied to the smear and set over a steaming water bath for 10 min (stain was no... Read More

Live/Dead Stain Microscopy

Tannery tanning fluid sample stained with Live/Dead BacLight under UV light. Either 40x or 100x oil immersion. Green bacteria indicate living cells, while Orange bacteria indicate dead cells. Some of the green cells were moving around even after staining! Read More

Starch Agar

Starch agar contains high molecular weight starch molecules (soluable starch). When Iodine is added it reacts with starch to produces a dark brown/purple color. A zone of clearing forms around organisms that produce the exoenzyme amylase after addition of iodine. This plate was incubated for ... Read More

Fluid Thioglycollate Media

Organisms grown in Fluid Thioglycollate Media w/out resazurin dye is a reducing media that uses sodium thioglycollate to chemically combine with dissolved oxygen to deplete the oxygen in the media. All organism except for Neisseria sicca were grown for 48 hours at 37 degrees C. Neisseria sicca i... Read More

Enterobacter aerogenes on MacConkey Agar (Mac)

Streak plate isolation of Enterobacter aerogenes grown on MacConkey agar for 24 hours at 37 degree's. Colonies grow pink due to fermentation of lactose. Read More

MaConkey Agar (Mac)

Organisms grown on MacConkey Agar at 37 degrees for 24 hours.
-Important Ingredients: crystal violet, bile salts, neutral red (color indicator), lactose
-Differential: used to identify and isolate lactose fermenting G- enteric bacteria mainly Enterobacteriaceae. Strong lactose fermentation pr... Read More

Salmonella grown on Salmonella-Shigella (S-S) Agar

The following picture shows the isolated black colonies of Salmonella on Salmonella-Shigella (S-S) Agar. S-S Agar is used as a selective and differential medium for the isolation of Salmonella and some Shigella species. The inclusion of bile salts is to inhibit gram-positive organisms. Ferric ci... Read More
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