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Happy Holidays!

Large TSA plate: yellowish organism is Staph aureus, red organism is Serratia marcescens. Grown for 24 hrs at 37 degrees C. Read More

Unknown - Identification Help Please

I am in need of some assistance in Identifying a bacteria species. I am unable to locate a match in Bergey's Manual. Our copy is 20 years out of date. I know the photo isnt great, it was taken with a cell phone. The colonies are hot pink and were cultured on TSA slant. It is gram + micrococci, i... Read More

Fluid Thioglycollate Media

Organisms grown in Fluid Thioglycollate Media w/out resazurin dye is a reducing media that uses sodium thioglycollate to chemically combine with dissolved oxygen to deplete the oxygen in the media. All organism except for Neisseria sicca were grown for 48 hours at 37 degrees C. Neisseria sicca i... Read More

Streptococcus pyogenes Gram Stain

Gram stain done on S. pyogenes showing the characteristic long G+ streptococcus chains. Culture grown on blood agar for 48 hrs at 37 degree’s C. Read More

Bacillus/Diplobacillus

Simple stain done on an unknown bacteria, showing feathery rhizoid growth on TSA after a 48 hr incubation at 37 degree’s C, isolated from a floor swab. Single bacillus and diplobacillus can be seen though out. Read More

Mycobacterium smegmatis using transmitted light

Isolated colony of Mycobacterium smegmatis grown on TSA for 96 hrs at 37 degrees C. Margin is lobate with an irregular-rhizoid form. Image taken using transmitted light. Read More

Chromobacterium violaceum, Staphylococcus aureus

Created by: Michelle Vu, Research Associate, BS Microbiology
Isolate: Chromobacterium violaceum, Staphylococcus aureus
Agar: Blood Agar
Incubation Conditions: 24 hours at 37C + 24 hours at Room Temperature

Pictured is Chromobacterium violaceum and Staphylococcus aureus shaped in a valentin... Read More

Pseudomonas aeruginosa with black extracellular pigment

Pseudomonas aeruginosa isolated from sputum into department of Microbiology - Riuniti Hospital Reggio Calabria .
This strain of Pseudomonas secretes brown/black extracellular pigment: pyomelanin.
Photo from D'Aleo Francesco archive. Read More

Have a Happy Bioluminescent Fourth of July!

In this post from my Microbiology/Education blog, I wish everyone a lovely day of food, fellowship, and fireworks. My laboratory brings you our own "fireworks" through bioluminescence! Read More

Antibiotic Producing Fungal Colony

This fungal colony, in association with a few others, was grown on SDA and appears to be an antibiotic producer. The compounds that it's producing appear orange and red. The colony grew and began producing compounds on the agar after five days incubation at 30 degree Celsius. This plate is part ... Read More

Lipid Agar

Lipid Plate/Tributyrin Agar used to test for an organisms ability to produce the exoenzyme lipase which breaks down the lipids in the agar creating a clear zone around the organism. (A) Serratia marcesens, lipid hydrolysis, indicated by a zone of clearing around the growing colony, as well as th... Read More

Slime producing Staphylococcus epidermidis on Congo Red agar

Slime production by Staphylococcus epidermidis on Congo Red agar; demonstrated by black colored colonies. Slime production is one of the most important virulence factors produced by Coagulase negative Staphylococci.

The colonies of slime non-producing strains remain pink to red.
Read More

Swarming Motility/ Proteus mirabilis

A sample of Proteus mirabilis was inoculated on to a TSA plate by taking a sterile swab and placing a drop in the center of the plate. The plate was then incubated at 37 degrees for 8 hrs. Swarming motility can be seen as a clear halo and finger like projections coming from and around the grow... Read More

Why are bacteria all the same size

Just when we think we know everything, a story comes along to remind us that there is something fundamental--and seemingly elementary--that we still haven't figured out. “Why are we the size we are? Why are our organs the size they are? Why are the cells in those organs a stereotypical size? Wha... Read More

Live/Dead Stain Microscopy

Tannery tanning fluid sample stained with Live/Dead BacLight under UV light. Either 40x or 100x oil immersion. Green bacteria indicate living cells, while Orange bacteria indicate dead cells. Some of the green cells were moving around even after staining! Read More

Hookworm egg

Shown here is a picture of a hookworm egg found on a concentrate in an ova & parasite exam

Human hookworms include the nematode species, Ancylostoma duodenale and Necator americanus. These species have indistinguishable eggs and can only be distinguished by their larvae.

Infective larvae g... Read More

Freshman Biology Creative Projects!

I have found that students become involved, energized, and enthusiastic when I give them opportunities to explore topics in my classes using their own creative approaches and interests. So this semester, in my Biology 111 course at the University of Puget Sound, I found that students explored f... Read More

Acid Fast Stain#2

Acid Fast stain done on a mix of Staphylococcus aureus and Mycobacterium smegmatis. The stain was done on a culture of M. smegmatis that was incubated on a TSA slant with 1ml TSB added at 37 degree's C for 4 days, S. aureus was incubated in TSB at 37 degree's C for 24 hrs. Several loops of eac... Read More

Macroconidia of Alternaria alternata

I have taken this microphotograph of macroconidia of Alternaria alternata.

Sheikh Imtiyaz
Department of microbiology
SKIMS Soura Srinagar India Read More

Streptomyces albus

Streptomycetes are a member actinomycete family. Streptomycetes are usually found in soil and are important decomposers. They are aerobic, gram-positive, filamentous rods that create branching hyphae. They also produce more than half of the world's antibiotics, and are therefore very valuable in... Read More
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