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Mycobacterium smegmatis using transmitted light

Isolated colony of Mycobacterium smegmatis grown on TSA for 96 hrs at 37 degrees C. Margin is lobate with an irregular-rhizoid form. Image taken using transmitted light. Read More

Streptococcus pyogenes on blood agar

Streak plate of Streptococcus pyogenes (incubated @ 37 degrees C for 48 hrs) showing beta hemolysis, complete lysis of red blood cells, around the isolated colonies. Image taken using transmitted light. Read More

DNase Agar

DNase agar can be used to differentiate between potentially pathogenic S. aureus and other Staphlococcus by looking for the presense of the exoenzyeme DNase. After growth for 24-48 hrs 1N HCL is added to the agar plate. Addition of HCL precipitates DNA in the media causing cloudiness unless t... Read More

Kirby-Bauer disk diffusion

Kirby-Bauer disk diffusion susceptibility test on coagulase-negative Staphylococcus aureus and E.coli grown on Mueller-Hinton agar with tetracycline (30 µg), cephalothin (30 µg), erythromycin (15 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), streptomycin (10 µg), and novo... Read More

Lipid Agar

Lipid Plate/Tributyrin Agar used to test for an organisms ability to produce the exoenzyme lipase which breaks down the lipids in the agar creating a clear zone around the organism. (A) Serratia marcesens, lipid hydrolysis, indicated by a zone of clearing around the growing colony, as well as th... Read More

Casein Media

Casein Plates contain the major milk protein found in milk. Organisms were grown on Casien agar for 72 hours at 37o C to look for the presence of the exoenzyme proteases/caseases . (A): Pseudomonas aeruginosa, casein hydrolysis, as noted by a zone of clearing around the organism, as well as a ... Read More

Micrococcus luteus line inoculation

Line inoculation of Micrococcus luteus on a TSA slant showing Filiform, uniform growth, along the margin. Culture was grown for 3 days at 37 degrees, M. luteus usually takes 3+ days for good growth. Read More

Teaching Freshmen about Transformation, Drug Resistance, and Gene Regulation!

In this blog post, I describe how I use the commonly available pGLO plasmid to teach freshman students basic concepts about transformation, receptor recognition, drug resistance mechanisms, and gene regulation. Oh, and fluorescence via GFP! Read More

Mycobacterium smegmatis line inoculation

Line inoculation of Mycobacterium smegmatis on a TSA slant showing friable, dry crusty, growth. Culture was grown for 3 days at 37 degrees. Read More

ASM workshop in Nepal " Scientific writing and publishing"

Nepal is a developing country, where there is more craziness increasing day by day in the field of microbiology.Microbes are remarkably diverse organisms. They live in habitats throughout the world: in fresh and salt water, on land, in the air, and on or inside other organisms. we can see smiley... Read More

"Microbe-Powered Jobs: How Microbiologists Can Help Build the Bioeconomy" Infographic

The American Academy of Microbiology has just released a new report, "Microbe-Powered Jobs: How Microbiologists Can Help Build the Bioeconomy," and along with it, an infographic, that summarizes the main points of the report. The full report can be found here: http://bit.ly/1lk346I, and a link t... Read More

Metallo-β-lactamase producing Acinetobacter species

Acinetobacter species are among the most common nosocomial pathogens that are notorious for multi-drug resistance. Their immense ability to acquire or upregulate antibiotic drug resistance determinants has established them as a successful pathogen, causing wide range of infections such as wound ... Read More

Yellowstone Caldera, Wyoming

Bacterial mats, golden brown in color, seen at the outer edges of Chromatic Pools. The blue water in the center is the hottest water at ~199 F and cooling to ambient the farther you get from the center. Cyanobacteria grow around 163 F or lower. Read More

Assortment in colony biofilms

Effect of founder cell density on the spatial pattern formation in colony biofilms.
The colony biofilms were obtained by following the protocol described in van Gestel et al (2014) ISME Journal (doi: 10.1038/ismej.2014.52). Briefly, GFP and mKATE2 labeled Bacillus subtilis strain were mixed in ... Read More

Bacterial Mat

Yellowstone caldera, Wyoming. Golden brown thermophilic cyano-bacteria seen in the waters surrounding one of the hydrothermal vents. Read More

Alcaligenes faecalis

Streak plate of Alcaligenes faecalis grown on TSA for 48 hr. Read More

Physarum polycephalum Different view

Physarum polycephalum, slime mold, grown in a large perti plate on moist paper towels using oatmeal as the food source. Culture was grown in the dark at room temperature. The paper towel was moistened every day with tap water. After 3 week’s the culture formed sporangia (fruiting bodies). Image ... Read More

Clostridium thermocellum

Monolayer biofilms of the Clostridium thermocellum bacterium (in situ and in vivo).

Credit: Mr. Alexandru Dumitrache
University of Toronto
Toronto, Canada

Olympus Bioscapes 2013 Honorable Mention. Read More

Tube morphology

Three organisms inoculated in TSB to look at tube morphology. From left to right:
Staphylococcus aureus: flocculent growth/turbid, growth throughout the tube.
Mycobacterium smegmatis: pellicle, growth at the top of the tube. M. smegmatis tends to stick to the tube and grow up the side.
Ba... Read More

Blood agar/Hemolysis

Three organisms inoculated onto blood agar, straight line inoculation, to demonstrate hemolysis. From top to bottom:
Streptococcus pyogenes: beta, complete lysis of red blood cells, clear area around colony growth.
Streptococcus bovis: alpha, incomplete lysis of red blood cells, green area ... Read More

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