Physarum polycephalum, slime mold, grown in a large perti plate on moist paper towels using oatmeal as the food source. Culture was grown in the dark at room temperature. The paper towel was moistened every day with tap water. After 3 week’s the culture formed sporangia (fruiting bodies). Image ... Read More
Organisms grown on MacConkey Agar at 37 degrees for 24 hours.
-Important Ingredients: crystal violet, bile salts, neutral red (color indicator), lactose
-Differential: used to identify and isolate lactose fermenting G- enteric bacteria mainly Enterobacteriaceae. Strong lactose fermentation pr... Read More
Kirby-Bauer disk diffusion susceptibility test on coagulase-negative Staphylococcus aureus and E.coli grown on Mueller-Hinton agar with tetracycline (30 µg), cephalothin (30 µg), erythromycin (15 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), streptomycin (10 µg), and novo... Read More
Important ingredients: 6.5% salt, dextrose, bromcresol purple
Differential/Selective: used to test for salt tolerance and can be used to identify enterococcal group D streptococci. NaCl is a selective agent and those organisms that can grow in higher salt concentrations also ferment the dextro... Read More
For my medical microbiology course at Kutztown University of Pennsylvania we were asked to take a sample from our nose. Using a sterile cotton swab, a sample was taken from a nostril and then spread onto the Mannitol Salt Agar plate supplied. The plate was incubated at 37 degrees Celsius, then e... Read More
Gram stain done on S. pyogenes showing the characteristic long G+ streptococcus chains. Culture grown on blood agar for 48 hrs at 37 degree’s C. Read More
The following picture shows the isolated black colonies of Salmonella on Salmonella-Shigella (S-S) Agar. S-S Agar is used as a selective and differential medium for the isolation of Salmonella and some Shigella species. The inclusion of bile salts is to inhibit gram-positive organisms. Ferric ci... Read More
This episode: Hoopoes inoculate their eggs with protective bacteria!
(6.5 MB, 7 minutes)
A simple stain done on a stock culture of Aquaspirillum serpens to look at cell shape. This culture was grown in TSB at room temperature, ~21 degrees C, for several months. Aquaspirillum is a slow grower normally found in pond water. A characteristic spiral rod can be seen in the center of ... Read More
Mannitol Salt Agar (MSA)
•Important Ingredients: Phenol red, mannitol (sugar), 7.5% NaCl
•Differential: between pathogenic S. aureus and S. epidermidis
•Selective: NaCl inhibits non-halophilic organisms. Organism that can grow are halophilic.
•Mannitol Fermentors: fermentation of acid cau... Read More
Pictured is a plant structure called a trichome that was found in a felines' urine sediment collected by cystocentesis. It is surrounded by many cocci and debris. The picture was taken with a smartphone at 400x magnification with no staining. Read More
Bacitracin test done on a lawn of Streptococcus pyogenes grown on blood agar. The zone of inhibition around the bacitracin disc, approx 14mm measuring the entire length of the zone, indicates sensitivity. The zone of inhibition is red because the red blood cells did not lyse. Grown for 24 hr... Read More
Line inoculation of Micrococcus luteus on a TSA slant showing Filiform, uniform growth, along the margin. Culture was grown for 3 days at 37 degrees, M. luteus usually takes 3+ days for good growth. Read More
Photograph of 400X magnified image of biogas digestor sample. A strange spiraled type microbe seen (probably like left handed DNA helix). The microbe is shown with an arrow. Has anyone seen such microbial structure? Read More
This image results from a project called "Initiation to microbiology investigation" accomplished by young students, where they collected a sample from a table surface and by inoculation they put it in CLED (cystine lactose electrolyte deficient) medium. Beautiful colonies of bacteria and fungu... Read More
The slide was prepared from blood of infected animals (cattle). The smear was stained with poly-chrome methylene blue which demonstrated blue colored bacillary body and light pink colored capsule (McFadyean's reaction).
The animal had the symptoms of high fever, convulsion and sudden death. ... Read More
Simple stain done on an unknown bacteria, showing feathery rhizoid growth on TSA after a 48 hr incubation at 37 degree’s C, isolated from a floor swab. Single bacillus and diplobacillus can be seen though out. Read More