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Aquaspirillum serpens

A simple stain done on a stock culture of Aquaspirillum serpens to look at cell shape. This culture was grown in TSB at room temperature, ~21 degrees C, for several months. Aquaspirillum is a slow grower normally found in pond water. A characteristic spiral rod can be seen in the center of ... Read More

Kirby-Bauer disk diffusion

Kirby-Bauer disk diffusion susceptibility test on coagulase-negative Staphylococcus aureus and E.coli grown on Mueller-Hinton agar with tetracycline (30 µg), cephalothin (30 µg), erythromycin (15 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), streptomycin (10 µg), and novo... Read More

6.5% NaCl Media

Important ingredients: 6.5% salt, dextrose, bromcresol purple
Differential/Selective: used to test for salt tolerance and can be used to identify enterococcal group D streptococci. NaCl is a selective agent and those organisms that can grow in higher salt concentrations also ferment the dextro... Read More

BacterioFiles 213 - Bird Bacteria Bolster Babies

This episode: Hoopoes inoculate their eggs with protective bacteria!


(6.5 MB, 7 minutes)


Show notes: 
News item/ Read More

fluorescent from Chicago River!

A group of students (Adrienne Linzemann, Jose Aveja, Elsa quintero), staff (William Thompson), and faculty (Farahnaz Movahedzadeh) from Harold Washington College participated in Chicago River interdisciplinary project with the leadership of VP Margie Martyn, and isolated Pseudomonas fluorescens ... Read More

Salmonella grown on Salmonella-Shigella (S-S) Agar

The following picture shows the isolated black colonies of Salmonella on Salmonella-Shigella (S-S) Agar. S-S Agar is used as a selective and differential medium for the isolation of Salmonella and some Shigella species. The inclusion of bile salts is to inhibit gram-positive organisms. Ferric ci... Read More

Streptococcus pyogenes Gram Stain

Gram stain done on S. pyogenes showing the characteristic long G+ streptococcus chains. Culture grown on blood agar for 48 hrs at 37 degree’s C. Read More

Vibrio cholerae serotype O1 ogawa isolated on TCBS agar in Sukraraj Tropical and Infectious Hospital , Kathmandu , Nepal .

Vibrio cholerae was isolated in the capital city Kathmandu , where as it causes cholerae endemic in certain remote parts in the country every year . Vibrio cholerae O1 ogawa serotype was isolated and identified after growth in TCBS agar and subcultured on HIA plate and performed oxidase test (+... Read More

Bacitracin test on Streptococcus pyogenes

Bacitracin test done on a lawn of Streptococcus pyogenes grown on blood agar. The zone of inhibition around the bacitracin disc, approx 14mm measuring the entire length of the zone, indicates sensitivity. The zone of inhibition is red because the red blood cells did not lyse. Grown for 24 hr... Read More

Fungus in my brothers sports drink

Unopened sports drink containing a fungal contaminant Read More

Bacillus/Diplobacillus

Simple stain done on an unknown bacteria, showing feathery rhizoid growth on TSA after a 48 hr incubation at 37 degree’s C, isolated from a floor swab. Single bacillus and diplobacillus can be seen though out. Read More

Lipid Agar

Lipid Plate/Tributyrin Agar used to test for an organisms ability to produce the exoenzyme lipase which breaks down the lipids in the agar creating a clear zone around the organism. (A) Serratia marcesens, lipid hydrolysis, indicated by a zone of clearing around the growing colony, as well as th... Read More

Trichome

Pictured is a plant structure called a trichome that was found in a felines' urine sediment collected by cystocentesis. It is surrounded by many cocci and debris. The picture was taken with a smartphone at 400x magnification with no staining. Read More

Beautiful image of different microbes in CLED medium

This image results from a project called "Initiation to microbiology investigation" accomplished by young students, where they collected a sample from a table surface and by inoculation they put it in CLED (cystine lactose electrolyte deficient) medium. Beautiful colonies of bacteria and fungu... Read More

Halloween microbes

Strep agalactiae (Group B Strep) streaked out on Granada plates. Usually this media is used to screen for Group B in pregnant patients, the strep will grow as bright orange colonies while any other growth will be colorless. Perfect for a little Halloween fun...follow me on instagram @stylish_str... Read More

D- Zone Test for phenotypic detection of Inducible clindamycin Resistance

A positive D test indicates that the presence of macrolide-inducible resistance to clindamycin produced by an inducible methylase enzyme that alters the common ribosomal binding site for macrolides, clindamycin and the group B streptogrammins (quinupristin). This cross-resistance, called the ML... Read More

Spiraled microbe

Photograph of 400X magnified image of biogas digestor sample. A strange spiraled type microbe seen (probably like left handed DNA helix). The microbe is shown with an arrow. Has anyone seen such microbial structure? Read More

Unknown - Identification Help Please

I am in need of some assistance in Identifying a bacteria species. I am unable to locate a match in Bergey's Manual. Our copy is 20 years out of date. I know the photo isnt great, it was taken with a cell phone. The colonies are hot pink and were cultured on TSA slant. It is gram + micrococci, i... Read More

Contaminant snowman

Some holiday fun from last winter composed of different collected plate contaminants. Based on colony morphology and wet mounts, these bacteria are likely to be: Staphylococcus sp. (white), Serratia marcescens (dark orange), and Micrococcus luteus (creamy yellow). The design was incubated for 5-... Read More

Streptococcus pyogenes

Throat swab of (5) years old child from emergency department for culture and sensitivity clinical summary scarlet fever.

Streptococcus pyogenes, or Group A Streptococcus, is a spherical, Gram-positive bacterium.[1] S. pyogenes displays streptococcal group A antigen on its cell wall and typic... Read More
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