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Bacillus subtilis

Streak plate isolation showing colonial morphology of Bacillus subtilis on trypticase soy agar (TSA) incubated for 24 hours at 37oC. Note the rough and dry/matte texture characteristic of this organism’s growth. Read More

Lactose fermenting E.coli after overnight growing on MacConkey agar

MacConkey agar plate with invasive lactose fermenting E.coli which was isolated for fatal case of neonatal septicemia.
Photo was taken after 12h incubation under 37C.
Credits: Povilas Kavaliauskas, Vilnius University, Lithuania. Read More

Methicillin-resistant Staphylococcus aureus

Scanning electron micrograph of methicillin-resistant Staphylococcus aureus and a dead human neutrophil. Credit: National Institutes of Health/Department of Health and Human Services (NIAID). Read More

Physarum polycephalum Different view

Physarum polycephalum, slime mold, grown in a large perti plate on moist paper towels using oatmeal as the food source. Culture was grown in the dark at room temperature. The paper towel was moistened every day with tap water. After 3 week’s the culture formed sporangia (fruiting bodies). Image ... Read More

Fungus in my brothers sports drink

Unopened sports drink containing a fungal contaminant Read More

MaConkey Agar (Mac)

Organisms grown on MacConkey Agar at 37 degrees for 24 hours.
-Important Ingredients: crystal violet, bile salts, neutral red (color indicator), lactose
-Differential: used to identify and isolate lactose fermenting G- enteric bacteria mainly Enterobacteriaceae. Strong lactose fermentation pr... Read More

Kirby-Bauer disk diffusion

Kirby-Bauer disk diffusion susceptibility test on coagulase-negative Staphylococcus aureus and E.coli grown on Mueller-Hinton agar with tetracycline (30 µg), cephalothin (30 µg), erythromycin (15 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), streptomycin (10 µg), and novo... Read More

S. aureus on MSA Plate

For my medical microbiology course at Kutztown University of Pennsylvania we were asked to take a sample from our nose. Using a sterile cotton swab, a sample was taken from a nostril and then spread onto the Mannitol Salt Agar plate supplied. The plate was incubated at 37 degrees Celsius, then e... Read More

fluorescent from Chicago River!

A group of students (Adrienne Linzemann, Jose Aveja, Elsa quintero), staff (William Thompson), and faculty (Farahnaz Movahedzadeh) from Harold Washington College participated in Chicago River interdisciplinary project with the leadership of VP Margie Martyn, and isolated Pseudomonas fluorescens ... Read More

Trichome

Pictured is a plant structure called a trichome that was found in a felines' urine sediment collected by cystocentesis. It is surrounded by many cocci and debris. The picture was taken with a smartphone at 400x magnification with no staining. Read More

Spiraled microbe

Photograph of 400X magnified image of biogas digestor sample. A strange spiraled type microbe seen (probably like left handed DNA helix). The microbe is shown with an arrow. Has anyone seen such microbial structure? Read More

6.5% NaCl Media

Important ingredients: 6.5% salt, dextrose, bromcresol purple
Differential/Selective: used to test for salt tolerance and can be used to identify enterococcal group D streptococci. NaCl is a selective agent and those organisms that can grow in higher salt concentrations also ferment the dextro... Read More

Beauty of Ecoli colony in EMB plate

Ecoli isolated from food sample in our laboratory Read More

Streptococcus pyogenes Gram Stain

Gram stain done on S. pyogenes showing the characteristic long G+ streptococcus chains. Culture grown on blood agar for 48 hrs at 37 degree’s C. Read More

Streptomyces albus

Streptomycetes are a member actinomycete family. Streptomycetes are usually found in soil and are important decomposers. They are aerobic, gram-positive, filamentous rods that create branching hyphae. They also produce more than half of the world's antibiotics, and are therefore very valuable in... Read More

BacterioFiles 213 - Bird Bacteria Bolster Babies

This episode: Hoopoes inoculate their eggs with protective bacteria!


(6.5 MB, 7 minutes)


Show notes: 
News item/ Read More

Beautiful image of different microbes in CLED medium

This image results from a project called "Initiation to microbiology investigation" accomplished by young students, where they collected a sample from a table surface and by inoculation they put it in CLED (cystine lactose electrolyte deficient) medium. Beautiful colonies of bacteria and fungu... Read More

Micrococcus luteus line inoculation

Line inoculation of Micrococcus luteus on a TSA slant showing Filiform, uniform growth, along the margin. Culture was grown for 3 days at 37 degrees, M. luteus usually takes 3+ days for good growth. Read More

Bacitracin test on Streptococcus pyogenes

Bacitracin test done on a lawn of Streptococcus pyogenes grown on blood agar. The zone of inhibition around the bacitracin disc, approx 14mm measuring the entire length of the zone, indicates sensitivity. The zone of inhibition is red because the red blood cells did not lyse. Grown for 24 hr... Read More

Salmonella grown on Salmonella-Shigella (S-S) Agar

The following picture shows the isolated black colonies of Salmonella on Salmonella-Shigella (S-S) Agar. S-S Agar is used as a selective and differential medium for the isolation of Salmonella and some Shigella species. The inclusion of bile salts is to inhibit gram-positive organisms. Ferric ci... Read More
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