Phenotypic test for detection of AmpC β-lactamases.
boronic acid is inhibitor of ampC β-lactamases. result: if zone of inhibition in Cefoxitin(FOX)+Boronic acid(BA) > Cefoxitin(FOX)=5MM.it indicates amp c producer. media :MHA organism: klebisella pneumoniae our resarch work on AmpC β-lactama... Read More
Organisms grown in Fluid Thioglycollate Media w/out resazurin dye is a reducing media that uses sodium thioglycollate to chemically combine with dissolved oxygen to deplete the oxygen in the media. All organism except for Neisseria sicca were grown for 48 hours at 37 degrees C. Neisseria sicca i... Read More
I am Sagar Aryal, Global Outreach Member of ASM, currently studying M.Sc. Medical Microbiology at St. Xavier's College, Kathmandu, Nepal.
This is the picture of presence of metallic sheen in m Endo Agar while detecting the coliforms in different water sample of kathmandu valley by membrane fil... Read More
On the first day of Micro class instructors have students do an environmental swab. This sample was subcultured from a student who did the bottom of their shoe. The organism, possible Bacillus sp., was tough to scrap off the agar so subculture was done by taking a small chunk of agar containi... Read More
Fluorescence staining of a direct sputum smear from a patient of chronic bronchitis showing numerous bacilli of Mycobacterium tuberculosis. Read More
An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the green and yellow grainy spore formation in the center of the colony with exudate ... Read More
An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the red/pink grainy spore formation in the center of the colony. This sample grew at ... Read More
"Clinically Important Drug-Resistant Bacteria” Read More
Vibrio cholerae was isolated in the capital city Kathmandu , where as it causes cholerae endemic in certain remote parts in the country every year . Vibrio cholerae O1 ogawa serotype was isolated and identified after growth in TCBS agar and subcultured on HIA plate and performed oxidase test (+... Read More
Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and an irregular-rhizoid form with lobate margins. The pigment is opaque white-beige with a mucoid surface over most of the colony. A dry, dull, matte surface appears toward the outer edges of the colony. The s... Read More
Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and mounding in an irregular-rhizoid form with lobate margins. The colony exhibited an opaque yellow-orange pigment with a mucoid surface. This sample was obtained from a floor swab and incubated at 37°C. Read More
Acinetobacter species are among the most common nosocomial pathogens that are notorious for multi-drug resistance. Their immense ability to acquire or upregulate antibiotic drug resistance determinants has established them as a successful pathogen, causing wide range of infections such as wound ... Read More
These clinical isolates are from Medinipur Medical College. Read More
In this blog post, I discuss our Summer Research Program's "Lab Themed Dessert" competion, for which one of my students created a cake "sculpture" of Bdellovibrio attacking and invading E. coli. It was tasty, informative, and fun! My student clearly thought about Bdellovibrio a LOT during the ... Read More
Growth of Acetobacter pasteurianus on glucose, yeast extract, calcium carbonate, ethanol medium.
Formation of acetic acid from ethanol oxidation is shown by a clear halo around bacterial growth due to dissolution of calcium carbonate.
Organism: Acetobacter pasteurianus. Incubation conditions:... Read More
Media is poured in bulk in our lab for classroom use. Although contamination is low we do see airborne contamination especially durning the rainy season. This is an unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth can be see... Read More
Entamoeba, an amoeba that includes pathogens of the intestinal tracts of a range of hosts - humans are included. They have no mitochondria and seem to have adapted secondarily to an anoxic way of life. Cytoplasm of a thick and dense consistency, and like that of pelobionts (to which we think the... Read More
Microorganisms are very diverse and include all the bacteria and archaea and almost all the protozoa. They also include some members of the fungi, algae, and animals such as rotifers. Many macro animals and plants have juvenile stages which are also microorganisms. Some microbiologists also clas... Read More
this isolate is v. cholerae o1,so colony pic of v. cholerae written as ASM(american society for microbiology) in TCBS(thiosulphate citrate bile salt sucrose) media as sucrose fermenting yellow colonies.
organisms: V. cholerae o1
media:TCBS(thiosulphate citrate bile salt sucrose) media
incub... Read More