Organisms grown in Fluid Thioglycollate Media w/out resazurin dye is a reducing media that uses sodium thioglycollate to chemically combine with dissolved oxygen to deplete the oxygen in the media. All organism except for Neisseria sicca were grown for 48 hours at 37 degrees C. Neisseria sicca i... Read More
E. coli was grown on MacConkey Agar (MAC) at 37 degrees for 24 hour. MAC is a Selective and Differential media used to inhibit G+ growth and some G- bacteria as well as identify and isolate lactose fermenting G- enteric bacteria mainly Enterobacteriaceae. Strong lactose fermentation produces pi... Read More
Acid Fast stain done on a mix of Staphylococcus aureus and Mycobacterium smegmatis. The stain was done on a culture of M. smegmatis that was incubated on a TSA slant with 1ml TSB added at 37 degree's C for 4 days, S. aureus was incubated in TSB at 37 degree's C for 24 hrs. Several loops of eac... Read More
Unlike human viruses that cause disease, yeast viruses do not cause any obvious illness in yeast. On the contrary, some viruses of yeast are beneficial and produce toxins that kill off competing yeast, allowing their host to thrive. We often think of viruses as agents of death and disease, but... Read More
It's true that I adore microbiology, and I am lucky to teach it each year to my micronauts. So it was time to commit. Here is my second "Microbial Supremacy" tattoo! The artwork is by Peggy Muddles (http://www.redbubble.com/people/thevexedmuddler/works/15659350-microbial-badass-tattoo-full-co... Read More
This episode: Bacteria are important for a good immune response to unadjuvanted influenza vaccines!
(14.6 MB, 16 minutes)
Methyl Red/Vogoes-Proskauer (MR-VP)
One medium used for two separate but related tests, identifying butanediol (VP) or acidic fermentation products (MR) as part of the IMViC test used in the identification of Enterobacteriaceae.
•Methyl Red (MR): detects the ability of an organism to produc... Read More
Streptomycetes are a member actinomycete family. Streptomycetes are usually found in soil and are important decomposers. They are aerobic, gram-positive, filamentous rods that create branching hyphae. They also produce more than half of the world's antibiotics, and are therefore very valuable in... Read More
In a healthy adult human body, most internal organs such as the brain, spleen, liver, and heart are devoid of microorganisms because the immune system keeps them in check. After human host death, however, the immune system falters and microorganisms proliferate throughout the body beginning in ... Read More
MacConkey agar plate with invasive lactose fermenting E.coli which was isolated for fatal case of neonatal septicemia.
Photo was taken after 12h incubation under 37C.
Credits: Povilas Kavaliauskas, Vilnius University, Lithuania. Read More
Serratia marcescens can form brilliant red colonies on LB agar due to the synthesis of the secondary metabolite prodigiosin. In the Microbiology teaching lab, we had students create pigment mutants of S. marcescens and these lovely shades of pink and white were collected for analysis. Read More
The slide was prepared from blood of infected animals (cattle). The smear was stained with poly-chrome methylene blue which demonstrated blue colored bacillary body and light pink colored capsule (McFadyean's reaction).
The animal had the symptoms of high fever, convulsion and sudden death. ... Read More
What do you get when you kiss a Petri dish? Bacteria and yeast colonies that grow into a pretty collage composed of different colors and morphologies. This is an LB agar plate incubated at room temperature for several days. Read More
A simple stain done on a stock culture of Aquaspirillum serpens to look at cell shape. This culture was grown in TSB at room temperature, ~21 degrees C, for several months. Aquaspirillum is a slow grower normally found in pond water. A characteristic spiral rod can be seen in the center of ... Read More
This is a picture of the Christmas tree in the University of Colorado Hospital Microbiology Department.
The tree is adorned with homemade culture plate ornaments (one for each staff member) and topped with a stuffed Staphylococcus aureus microbe. Read More
"Two types of bacteria found in the soil have enabled scientists to get the dirt on how resistance to antibiotics develops along with a separate survival strategy.The study identifies an atypical antibiotic molecule and the way in which the resistance to that molecule arises, including the ident... Read More
This episode: Hoopoes inoculate their eggs with protective bacteria!
(6.5 MB, 7 minutes)
Important ingredients: 6.5% salt, dextrose, bromcresol purple
Differential/Selective: used to test for salt tolerance and can be used to identify enterococcal group D streptococci. NaCl is a selective agent and those organisms that can grow in higher salt concentrations also ferment the dextro... Read More
Gram stain done on S. pyogenes showing the characteristic long G+ streptococcus chains. Culture grown on blood agar for 48 hrs at 37 degree’s C. Read More
A group of students (Adrienne Linzemann, Jose Aveja, Elsa quintero), staff (William Thompson), and faculty (Farahnaz Movahedzadeh) from Harold Washington College participated in Chicago River interdisciplinary project with the leadership of VP Margie Martyn, and isolated Pseudomonas fluorescens ... Read More