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Large TSA plate: yellowish organism is Staph aureus, red organism is Serratia marcescens. Grown for 24 hrs at 37 degrees C. Read More

Alcaligenes faecalis

Streak plate of Alcaligenes faecalis grown on TSA for 48 hr. Read More

Kirby-Bauer disk diffusion

Kirby-Bauer disk diffusion susceptibility test on coagulase-negative Staphylococcus aureus and E.coli grown on Mueller-Hinton agar with tetracycline (30 µg), cephalothin (30 µg), erythromycin (15 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), streptomycin (10 µg), and novo... Read More

Bacillus subtilis

Streak plate isolation showing colonial morphology of Bacillus subtilis on trypticase soy agar (TSA) incubated for 24 hours at 37oC. Note the rough and dry/matte texture characteristic of this organism’s growth. Read More

Pseudomonas paints a picture!

A student and I painted with bacteria and this is what happened! We used a method similar to that used by artist Sarah Roberts (UK) and microbiologist Simon Park.

Motility agar plates were prepared and painted with watercolor paint before inoculation with P. aeruginosa. After 7 days at room t... Read More

Love and war between kingdoms

Candida albicans and Pseudomona aeruginosa growing on YPD. Read More

Micrococcus luteus

Micrococcus luteus grown on TSA for 96 hrs. Read More

Have a Happy Bioluminescent Fourth of July!

In this post from my Microbiology/Education blog, I wish everyone a lovely day of food, fellowship, and fireworks. My laboratory brings you our own "fireworks" through bioluminescence! Read More

Mycobacterium smegmatis using transmitted light

Isolated colony of Mycobacterium smegmatis grown on TSA for 96 hrs at 37 degrees C. Margin is lobate with an irregular-rhizoid form. Image taken using transmitted light. Read More

Swarming Motility of Pseudomonas aeruginosa

Strain: Pseudomonas aeruginosa PA14
Medium: M8 minimal medium agar plates
Incubation Conditions: 48 hours at 37°C
Pseudomonas aeruginosa is a gram-negative, rod-shaped and monoflagellated bacterium that has an incredible nutritional versatility. Swarming motility, a flagellum-dependent behavi... Read More

Methyl Red/Vogoes-Proskauer (MR-VP)

Methyl Red/Vogoes-Proskauer (MR-VP)
One medium used for two separate but related tests, identifying butanediol (VP) or acidic fermentation products (MR) as part of the IMViC test used in the identification of Enterobacteriaceae.

•Methyl Red (MR): detects the ability of an organism to produc... Read More

Micrococcus luteus line inoculation

Line inoculation of Micrococcus luteus on a TSA slant showing Filiform, uniform growth, along the margin. Culture was grown for 3 days at 37 degrees, M. luteus usually takes 3+ days for good growth. Read More

Streptococcus pyogenes Gram Stain

Gram stain done on S. pyogenes showing the characteristic long G+ streptococcus chains. Culture grown on blood agar for 48 hrs at 37 degree’s C. Read More

Chromobacterium violaceum, Staphylococcus aureus

Created by: Michelle Vu, Research Associate, BS Microbiology
Isolate: Chromobacterium violaceum, Staphylococcus aureus
Agar: Blood Agar
Incubation Conditions: 24 hours at 37C + 24 hours at Room Temperature

Pictured is Chromobacterium violaceum and Staphylococcus aureus shaped in a valentin... Read More

Pseudomonas aeruginosa with black extracellular pigment

Pseudomonas aeruginosa isolated from sputum into department of Microbiology - Riuniti Hospital Reggio Calabria .
This strain of Pseudomonas secretes brown/black extracellular pigment: pyomelanin.
Photo from D'Aleo Francesco archive. Read More

New drugs for bad bugs

Washington University in St Louis chemist Timothy Wencewicz says we’ll stay ahead of antibiotic resistance only if we find drugs with new scaffolds, or core chemical structures. One promising candidate, an antibiotic made by a bacterium than infects plants, caught his attention because it contai... Read More

Serratia marcescens/Escherichia coli mix

Serratia marcescens/Escherichia coli mix grown on TSA for 48 hrs. Serratia marcescens grows red due to prodigiosin and Escherichia coli grows opaque white. Read More

Live/Dead Stain Microscopy

Tannery tanning fluid sample stained with Live/Dead BacLight under UV light. Either 40x or 100x oil immersion. Green bacteria indicate living cells, while Orange bacteria indicate dead cells. Some of the green cells were moving around even after staining! Read More

Why are bacteria all the same size

Just when we think we know everything, a story comes along to remind us that there is something fundamental--and seemingly elementary--that we still haven't figured out. “Why are we the size we are? Why are our organs the size they are? Why are the cells in those organs a stereotypical size? Wha... Read More

Fluid Thioglycollate Media

Organisms grown in Fluid Thioglycollate Media w/out resazurin dye is a reducing media that uses sodium thioglycollate to chemically combine with dissolved oxygen to deplete the oxygen in the media. All organism except for Neisseria sicca were grown for 48 hours at 37 degrees C. Neisseria sicca i... Read More
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