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Alternaria change DTM agar colour

Alternaria and other demaziacee normally not change the DTM agar plates colour. This Alternaria isolated into Mycology Laboratory in Policlinic "G. Martino" - University of Messina, change the color on DTM agar. Read More

Demonstration of screening of Organic Acid producing Fungi.

Fungal Love......
Demonstration of screening of Organic Acid producing Fungi. Read More

Catching Up, Part I: Meeting with Former Research Students at ASM in Boston in May.

In this post, I meet up with six of my former undergraduate students at the General Meeting of the American Society of Microbiology in Boston last May. We had a great dinner where we could catch up on challenges and cheer each other on. I am very proud of the great students in this post, who a... Read More

Fluoribacter bozemanae expressing blue-white fluorescence under long-wave UV light.

Fluoribacter bozemanae expressing blue-white fluorescence under long-wave UV light. If you look carefully one of the steaks does not fluoresce on the right hand side of the plate. The dark colony is actually Legionella pneumophila. Read More

colony picture of dermatophyte

by: cls. sundar khadka,
PG in clinical microbiology,
institute of medicine(IOM), TU teaching hospital , kathmandu, nepal. Read More

Borrelia hermsii Bacteria

Scanning electron micrograph of Borrelia hermsii, the causative agent of relapsing fever, interacting with red blood cells.

Credit: National Institute of Allergy and Infectious Diseases (NIAID) Read More

Antibiotic Susceptibility Test (AST) Disc in Blood sample

I am sunil pandey from kathmandu Nepal, studying B.Sc in medical microbiology at nobel college. this is AST disc in MHA from blood sample, shows the clear zone of inhibition(Kirby -Bauer disc diffusion Method). I have done this experiment as part of my study "susceptibility of antibiotics in ba... Read More

Unknown Fungal Contaminant

Unknown fungal contaminant found on TSA. TSA plate was incubated at 4 degrees for two months after contaminant was discovered. Read More

Environmental Swab/Bottom of Shoe #1

On the first day of Micro class instructors have students do an environmental swab. This sample was subcultured from a student who did the bottom of their shoe. The organism, possible Bacillus sp., was tough to scrap off the agar so subculture was done by taking a small chunk of agar containi... Read More

D- Zone Test for phenotypic detection of Inducible clindamycin Resistance

A positive D test indicates that the presence of macrolide-inducible resistance to clindamycin produced by an inducible methylase enzyme that alters the common ribosomal binding site for macrolides, clindamycin and the group B streptogrammins (quinupristin). This cross-resistance, called the ML... Read More

Symbol Of ASM

This study done at department of Clinical Microbiology, TU Teaching Hospital , Kathamandu, Nepal.
Clinical specimen: Sputum
Media: Muller Hinton Agar
Incubation condition: at 37'c for 24hrs.
Isolate: Pseudomonas aerugionsa
Pic: Symbol of ASM.

Pseudomonas aeruginosa is a Gram-negative rod... Read More

Clear halo in petri dishes by Acetobacter pasteurianus

Growth of Acetobacter pasteurianus on glucose, yeast extract, calcium carbonate, ethanol medium.
Formation of acetic acid from ethanol oxidation is shown by a clear halo around bacterial growth due to dissolution of calcium carbonate.
Organism: Acetobacter pasteurianus. Incubation conditions:... Read More

Unknown floor isolate

Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and an irregular-rhizoid form with lobate margins. The pigment is opaque white-beige with a mucoid surface over most of the colony. A dry, dull, matte surface appears toward the outer edges of the colony. The s... Read More

colony of candida spp.

Colonies of candida species:

Media: HiCrome Candida Differential Agar
principle: Perry and Miller (1) reported that Candida albicans produces an enzyme b -N-acetyl- galactosaminidase and according to
Rousselle et al (2) incorporation of chromogenic or fluorogenic hexosaminidase substrates... Read More

Acid Fast Stain#2

Acid Fast stain done on a mix of Staphylococcus aureus and Mycobacterium smegmatis. The stain was done on a culture of M. smegmatis that was incubated on a TSA slant with 1ml TSB added at 37 degree's C for 4 days, S. aureus was incubated in TSB at 37 degree's C for 24 hrs. Several loops of eac... Read More

Unknown Floor isolate #2

Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and mounding in an irregular-rhizoid form with lobate margins. The colony exhibited an opaque yellow-orange pigment with a mucoid surface. This sample was obtained from a floor swab and incubated at 37°C. Read More

Life Under the Microscope: The Year’s Best Biology Close-Ups

Life is pretty interesting, and at the microscopic scale, it can also be beautiful, strange, intriguing, frightening and gross. The winning photos and videos from this year’s Olympus BioScapes competition span the whole range.

From rat brains to butter daisies to weevils and barnacle appendag... Read More

Microbiology and Molecular Genetics UT Health Christmas Tree

This is the of the Department of Microbiology and Molecular Genetics at UT Health Science Center Houston. It features commonly found in research laboratories supplies and equipment. Read More

Airborne Environmental Isolate #2

An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the green and yellow grainy spore formation in the center of the colony with exudate ... Read More

Gelatin Hydrolysis

Pseudomonas aeruginosa, Escherichia coli and Serratia marcescens were inoculated into gelatin agar using the stab method and incubated for 1 week at 37 degree’s C. If the organism has gelatinase then it can break down the gelatin (protein) into polypeptides and then amino acids which can then ... Read More
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