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Budding Yeast

I had a pink fungal contaminant on one of my plates.
When I looked at it under the microscope, I found some budding yeast. Read More

Fluorescence staining of direct sputum smear showing numerous bacilli of Mycobacteria.

Fluorescence staining of a direct sputum smear from a patient of chronic bronchitis showing numerous bacilli of Mycobacterium tuberculosis. Read More

Environmental Swab/Bottom of Shoe #1

On the first day of Micro class instructors have students do an environmental swab. This sample was subcultured from a student who did the bottom of their shoe. The organism, possible Bacillus sp., was tough to scrap off the agar so subculture was done by taking a small chunk of agar containi... Read More

Osmotic pressure

A) Bacillus subtilis, (B) Staphlococcus aureus and (C) Escherichia coli were grown on BHI media with varying concentrations of NaCl (0.85%, 5%, 7.5%, 10%, and 20%) at 37 degrees C for 24 hrs. Read More

colony picture of dermatophyte

by: cls. sundar khadka,
PG in clinical microbiology,
institute of medicine(IOM), TU teaching hospital , kathmandu, nepal. Read More

Unknown Fungal Contaminant

Unknown fungal contaminant found on TSA. TSA plate was incubated at 4 degrees for two months after contaminant was discovered. Read More

Airborne Environmental Isolate/Fungal view 2

Different view of an unknown airborne environmental isolate on Bile Esculin Agar (BEA) exhibiting a single circular colony. White hyphal growth with no spore formation. Clear to brownish exudate can be seen throughout the colony. This sample grew at refrigerated temperatures for several months.... Read More

Fluoribacter bozemanae expressing blue-white fluorescence under long-wave UV light.

Fluoribacter bozemanae expressing blue-white fluorescence under long-wave UV light. If you look carefully one of the steaks does not fluoresce on the right hand side of the plate. The dark colony is actually Legionella pneumophila. Read More

Antibiotic Susceptibility Test (AST) Disc in Blood sample

I am sunil pandey from kathmandu Nepal, studying B.Sc in medical microbiology at nobel college. this is AST disc in MHA from blood sample, shows the clear zone of inhibition(Kirby -Bauer disc diffusion Method). I have done this experiment as part of my study "susceptibility of antibiotics in ba... Read More

MHT positive or negative

On the left is test isolate and on right side is positive control. Imipenem MIC by etest method was 0.75 ug/ml. How to interpret this modified hodge test; positive or negative? Read More

colony of candida spp.

Colonies of candida species:

Media: HiCrome Candida Differential Agar
principle: Perry and Miller (1) reported that Candida albicans produces an enzyme b -N-acetyl- galactosaminidase and according to
Rousselle et al (2) incorporation of chromogenic or fluorogenic hexosaminidase substrates... Read More

Unknown floor isolate

Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and an irregular-rhizoid form with lobate margins. The pigment is opaque white-beige with a mucoid surface over most of the colony. A dry, dull, matte surface appears toward the outer edges of the colony. The s... Read More

Borrelia hermsii Bacteria

Scanning electron micrograph of Borrelia hermsii, the causative agent of relapsing fever, interacting with red blood cells.

Credit: National Institute of Allergy and Infectious Diseases (NIAID) Read More

Nocardia asteroides on Gram stain

Presence of Gram-positive, partially acid-fast rods, which have grown in branching chains resembling fungal hyphae. (Gram stain; original magnification, ×100). Image courtesy MicrobeWorld user Kyriakos Zaragkoulias, Specialty Registrar (StR) in Medical Microbiology at General Hospital of Thessal... Read More

Unknown Floor isolate #2

Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and mounding in an irregular-rhizoid form with lobate margins. The colony exhibited an opaque yellow-orange pigment with a mucoid surface. This sample was obtained from a floor swab and incubated at 37°C. Read More

Clear halo in petri dishes by Acetobacter pasteurianus

Growth of Acetobacter pasteurianus on glucose, yeast extract, calcium carbonate, ethanol medium.
Formation of acetic acid from ethanol oxidation is shown by a clear halo around bacterial growth due to dissolution of calcium carbonate.
Organism: Acetobacter pasteurianus. Incubation conditions:... Read More

Airborne Environmental Isolate #2

An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the green and yellow grainy spore formation in the center of the colony with exudate ... Read More

Airborne Environmental Isolate #3

An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the red/pink grainy spore formation in the center of the colony. This sample grew at ... Read More

Unknown Fungal Contaminant/View 1

Unknown airborne fungal isolated contaminant found on BEA. BEA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Spore formation can be seen throughout the circular colony. The single colony covered ¼ of the plate. Read More

Alternaria change DTM agar colour

Alternaria and other demaziacee normally not change the DTM agar plates colour. This Alternaria isolated into Mycology Laboratory in Policlinic "G. Martino" - University of Messina, change the color on DTM agar. Read More

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