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Environmental Swab/Bottom of Shoe #1

On the first day of Micro class instructors have students do an environmental swab. This sample was subcultured from a student who did the bottom of their shoe. The organism, possible Bacillus sp., was tough to scrap off the agar so subculture was done by taking a small chunk of agar containi... Read More

Osmotic pressure

A) Bacillus subtilis, (B) Staphlococcus aureus and (C) Escherichia coli were grown on BHI media with varying concentrations of NaCl (0.85%, 5%, 7.5%, 10%, and 20%) at 37 degrees C for 24 hrs. Read More

colony picture of dermatophyte

by: cls. sundar khadka,
PG in clinical microbiology,
institute of medicine(IOM), TU teaching hospital , kathmandu, nepal. Read More

Airborne Environmental Isolate/Fungal view 2

Different view of an unknown airborne environmental isolate on Bile Esculin Agar (BEA) exhibiting a single circular colony. White hyphal growth with no spore formation. Clear to brownish exudate can be seen throughout the colony. This sample grew at refrigerated temperatures for several months.... Read More

Unknown Fungal Contaminant

Unknown fungal contaminant found on TSA. TSA plate was incubated at 4 degrees for two months after contaminant was discovered. Read More

Fluoribacter bozemanae expressing blue-white fluorescence under long-wave UV light.

Fluoribacter bozemanae expressing blue-white fluorescence under long-wave UV light. If you look carefully one of the steaks does not fluoresce on the right hand side of the plate. The dark colony is actually Legionella pneumophila. Read More

Antibiotic Susceptibility Test (AST) Disc in Blood sample

I am sunil pandey from kathmandu Nepal, studying B.Sc in medical microbiology at nobel college. this is AST disc in MHA from blood sample, shows the clear zone of inhibition(Kirby -Bauer disc diffusion Method). I have done this experiment as part of my study "susceptibility of antibiotics in ba... Read More

colony of candida spp.

Colonies of candida species:

Media: HiCrome Candida Differential Agar
principle: Perry and Miller (1) reported that Candida albicans produces an enzyme b -N-acetyl- galactosaminidase and according to
Rousselle et al (2) incorporation of chromogenic or fluorogenic hexosaminidase substrates... Read More

Unknown floor isolate

Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and an irregular-rhizoid form with lobate margins. The pigment is opaque white-beige with a mucoid surface over most of the colony. A dry, dull, matte surface appears toward the outer edges of the colony. The s... Read More

Borrelia hermsii Bacteria

Scanning electron micrograph of Borrelia hermsii, the causative agent of relapsing fever, interacting with red blood cells.

Credit: National Institute of Allergy and Infectious Diseases (NIAID) Read More

Unknown Floor isolate #2

Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and mounding in an irregular-rhizoid form with lobate margins. The colony exhibited an opaque yellow-orange pigment with a mucoid surface. This sample was obtained from a floor swab and incubated at 37°C. Read More

Airborne Environmental Isolate #2

An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the green and yellow grainy spore formation in the center of the colony with exudate ... Read More

Clear halo in petri dishes by Acetobacter pasteurianus

Growth of Acetobacter pasteurianus on glucose, yeast extract, calcium carbonate, ethanol medium.
Formation of acetic acid from ethanol oxidation is shown by a clear halo around bacterial growth due to dissolution of calcium carbonate.
Organism: Acetobacter pasteurianus. Incubation conditions:... Read More

Airborne Environmental Isolate #3

An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the red/pink grainy spore formation in the center of the colony. This sample grew at ... Read More

Clinically Important Drug-Resistant Bacteria” .

"Clinically Important Drug-Resistant Bacteria” Read More

Unknown Fungal Contaminant/View 1

Unknown airborne fungal isolated contaminant found on BEA. BEA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Spore formation can be seen throughout the circular colony. The single colony covered ¼ of the plate. Read More

Gelatin Hydrolysis

Pseudomonas aeruginosa, Escherichia coli and Serratia marcescens were inoculated into gelatin agar using the stab method and incubated for 1 week at 37 degree’s C. If the organism has gelatinase then it can break down the gelatin (protein) into polypeptides and then amino acids which can then ... Read More

Growth on Manital Salt Agar of Staphylococcus aureus and staphylococcus epidermis isolated from wounds

These clinical isolates are from Medinipur Medical College. Read More

Metallo-β-lactamase producing Acinetobacter species

Acinetobacter species are among the most common nosocomial pathogens that are notorious for multi-drug resistance. Their immense ability to acquire or upregulate antibiotic drug resistance determinants has established them as a successful pathogen, causing wide range of infections such as wound ... Read More

D- Zone Test for phenotypic detection of Inducible clindamycin Resistance

A positive D test indicates that the presence of macrolide-inducible resistance to clindamycin produced by an inducible methylase enzyme that alters the common ribosomal binding site for macrolides, clindamycin and the group B streptogrammins (quinupristin). This cross-resistance, called the ML... Read More

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