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MaConkey Agar (Mac)

Organisms grown on MacConkey Agar at 37 degrees for 24 hours.
-Important Ingredients: crystal violet, bile salts, neutral red (color indicator), lactose
-Differential: used to identify and isolate lactose fermenting G- enteric bacteria mainly Enterobacteriaceae. Strong lactose fermentation pr... Read More

Salmonella grown on Salmonella-Shigella (S-S) Agar

The following picture shows the isolated black colonies of Salmonella on Salmonella-Shigella (S-S) Agar. S-S Agar is used as a selective and differential medium for the isolation of Salmonella and some Shigella species. The inclusion of bile salts is to inhibit gram-positive organisms. Ferric ci... Read More

E.coli on MacConkey Agar (Mac)

Streak plate isolation of E. coli on MacConkey Agar grown for 24 hrs at 37 degrees. E. coli demonstrates strong lactose fermentation indicated by the bright pink halo, bile precipitant around the colonies, and pink colony growth. Read More

ASM GM 2014 - Where Pathogens Can Linger on Airplanes

Many air travelers are concerned about the risks of catching a disease from other passengers given the long time spent in crowded air cabins. New research shows disease-causing bacteria can linger on surfaces commonly found in airplane cabins for days, even up to a week. Participants discuss ... Read More

Unknown Agar Contaminate

Unknown contaminant found on a TSA plate after being in the refrigerator for 1 month. Organism had grown throughout the media, was pale yellow and mucoid. Read More

Mannitol Salt Agar (MSA)

Mannitol Salt Agar (MSA)
•Important Ingredients: Phenol red, mannitol (sugar), 7.5% NaCl
•Differential: between pathogenic S. aureus and S. epidermidis
•Selective: NaCl inhibits non-halophilic organisms. Organism that can grow are halophilic.
•Mannitol Fermentors: fermentation of acid cau... Read More

Mannitol Salt Agar (MSA)

Mannitol Salt Agar (MSA)
•Important Ingredients: Phenol red, mannitol (sugar), 7.5% NaCl
•Differential: between pathogenic S. aureus and S. epidermidis
•Selective: NaCl inhibits non-halophilic organisms. Organism that can grow are halophilic.
•Mannitol Fermentors: fermentation of acid cau... Read More

E. coli colonies on Eosin Methylene Blue agar plate

Isolates colonies of E. coli with distinct green metallic sheen on EMB agar plate. Culture isolated from drinking water sample Read More

Bacitracin test on Streptococcus pyogenes

Bacitracin test done on a lawn of Streptococcus pyogenes grown on blood agar. The zone of inhibition around the bacitracin disc, approx 14mm measuring the entire length of the zone, indicates sensitivity. The zone of inhibition is red because the red blood cells did not lyse. Grown for 24 hr... Read More

Streptococcus mitis on blood agar

Growth of Streptococcus mitis on blood agar demonstrating alpha hemolysis seen as a greenish color around the growing colonies due to a reduction of the hemoglobin to methemoglobin in the surrounding agar. Image taken using transmitted light. Read More

Freshman Biology Creative Projects!

I have found that students become involved, energized, and enthusiastic when I give them opportunities to explore topics in my classes using their own creative approaches and interests. So this semester, in my Biology 111 course at the University of Puget Sound, I found that students explored f... Read More

Where Biology and Art Work Together in the Classroom

In this blog entry, I describe how working with the artist Katie McKissick ("Beatrice the Biologist") helped improve my freshman biology course. The intersection of biology and art benefits both! Read More

Growth on Manital Salt Agar of Staphylococcus aureus and staphylococcus epidermis isolated from wounds

These clinical isolates are from Medinipur Medical College. Read More

Mycobacterium smegmatis using transmitted light

Isolated colony of Mycobacterium smegmatis grown on TSA for 96 hrs at 37 degrees C. Margin is lobate with an irregular-rhizoid form. Image taken using transmitted light. Read More

Streptococcus pyogenes on blood agar

Streak plate of Streptococcus pyogenes (incubated @ 37 degrees C for 48 hrs) showing beta hemolysis, complete lysis of red blood cells, around the isolated colonies. Image taken using transmitted light. Read More

DNase Agar

DNase agar can be used to differentiate between potentially pathogenic S. aureus and other Staphlococcus by looking for the presense of the exoenzyeme DNase. After growth for 24-48 hrs 1N HCL is added to the agar plate. Addition of HCL precipitates DNA in the media causing cloudiness unless t... Read More

Kirby-Bauer disk diffusion

Kirby-Bauer disk diffusion susceptibility test on coagulase-negative Staphylococcus aureus and E.coli grown on Mueller-Hinton agar with tetracycline (30 µg), cephalothin (30 µg), erythromycin (15 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), streptomycin (10 µg), and novo... Read More

Lipid Agar

Lipid Plate/Tributyrin Agar used to test for an organisms ability to produce the exoenzyme lipase which breaks down the lipids in the agar creating a clear zone around the organism. (A) Serratia marcesens, lipid hydrolysis, indicated by a zone of clearing around the growing colony, as well as th... Read More

Casein Media

Casein Plates contain the major milk protein found in milk. Organisms were grown on Casien agar for 72 hours at 37o C to look for the presence of the exoenzyme proteases/caseases . (A): Pseudomonas aeruginosa, casein hydrolysis, as noted by a zone of clearing around the organism, as well as a ... Read More

Micrococcus luteus line inoculation

Line inoculation of Micrococcus luteus on a TSA slant showing Filiform, uniform growth, along the margin. Culture was grown for 3 days at 37 degrees, M. luteus usually takes 3+ days for good growth. Read More

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