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10/23/2014
Cyanine5 NHS ester http://www.lumiprobe.com/p/cy5-nhs-ester During the last few years, Cyanine5 flurophore (analog of Cy5) has become an incredibly popular label of amino-groups in peptides, proteins, and oligonucleotides. Fluorophore emission has maximum in red region, where many CCD detectors have maximum sensitivity, and biological objects have low background. Dye color is very ...
10/23/2014
Cyanine5 NHS ester http://www.lumiprobe.com/p/cy5-nhs-ester During the last few years, Cyanine5 flurophore (analog of Cy5) has become an incredibly popular label of amino-groups in peptides, proteins, and oligonucleotides. Fluorophore emission has maximum in red region, where many CCD detectors have maximum sensitivity, and biological objects have low background. Dye color is very ...

BDP FL maleimide - BODIPY analog - fluorescent labeling of proteins

09/30/2014
BDP FL maleimide http://www.lumiprobe.com/p/bodipy-fl-maleimide BDP FL maleimide is a bright and photostable thiol-reactive dye for protein labeling, an ideal replacement for fluorescein for microscopy. BDP FL is a borondipyrromethene dye which has absorption and fluorescence spectra similar to fluorescein (FAM). However, this dye exhibits very high photostability. It is non-charged, and has ...

Click-Chemistry Labeling of Oligonucleotides and DNA - Lumiprobe - Protocol

03/20/2014
Click chemistry is a versatile reaction that can be used for the synthesis of a variety of conjugates. Virtually any biomolecules can be involved, and labeling with small molecules, such as fluorescent dyes, biotin, and other groups can be readily achieved. Click chemistry reaction takes place between two components: azide and ...

Cyanine dyes - Non-sulfonated and Sulfonated cyanines Lumiprobe explains

01/30/2014
Sulfonated and non-sulfonated cyanines exhibit very similar fluorescent properties. Non-sulfonated cyanines must be dissolved in organic co-solvent (DMF or DMSO) prior to use, and added to a solution of target molecule in aqueous buffers. Recommended volume of co-solvent should be 10% for Cy3, Cy5, Cy7, and 15% for .5 counterparts. ...

How to use Fluorescent dyes or Click Chemistry in research

06/04/2013
Protocols and free how to advice from Lumiprobe on how to use Fluorescent dyes, Click Chemistry reagents, Life Science -Click-chemistry labeling of oligonucleotides and DNA. -NHS ester labeling of amino biomolecules. -SYBR Green I staining of DNA in gels. ...

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