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Sulfonated and non-sulfonated cyanines exhibit very similar fluorescent properties. Non-sulfonated cyanines must be dissolved in organic co-solvent (DMF or DMSO) prior to use, and added to a solution of target molecule in aqueous buffers. Recommended volume of co-solvent should be 10% for Cy3, Cy5, Cy7, and 15% for .5 counterparts. ...
Protocols and free how to advice from Lumiprobe on how to use Fluorescent dyes, Click Chemistry reagents, Life Science -Click-chemistry labeling of oligonucleotides and DNA. -NHS ester labeling of amino biomolecules. -SYBR Green I staining of DNA in gels. ...