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Tasha - Submissions

Articles

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Unknown Bacillus/Hand print

11/11/2016
Unknown organism, possible Bacillus spp, seen on a hand print after hand washing. TSA plate was grown at 37 C for 24 hours then left at refrigerated temps for 4 weeks. Organism was a light brown and mat. The center of the growth was smooth with rhizoid like growth on ...

Unknown Bacillus

11/11/2016
Unknown organism, possible Bacillus spp, seen on a hand print after hand washing. TSA plate was grown at 37 C for 24 hours then left at refrigerated temps for 4 weeks. Organism was a light brown and mat. The center of the growth was smooth with rhizoid like growth on ...

Finger bacteria

11/06/2016
Colorful cluster of colonies seen on a print from a finger grown on TSA at 37 degrees C for 48 hrs.

Bacillus mycodies

11/06/2016
Bacillus mycodies seen from an environmental swab grown on TSA at 37 degrees C for 49 hrs. Characteristic swirling rhizoid growth can be see.

Proteus mirabilis on Blood Agar

11/06/2016
A sample of Proteus mirabilis was inoculated on to a blood agar plate by taking a sterile swab and placing a drop in the center of the plate. The plate was then incubated at 37 degrees for 8 hrs. Swarming motility can be seen as a halo around the ...

Physarum

10/19/2016
Physarum polycephalum, slime mold, grown in a large perti plate on moist paper towels using oatmeal as the food source. Culture was grown in the dark at room temperature. The paper towel was moistened every day with tap water. After 3 week’s the culture formed sporangia (fruiting bodies). Image was ...

Oxidase Test

10/18/2016
The oxidase test is performed using a reducing agent to detect bacteria’s ability to produce cytochrome c oxidase, an enzyme in the electron transport chain. The reducing agent (N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD)) changes or produces a purple color as it become oxidized. Pseudomoonas aeruginosa: pruple color formed indicating ...

Catalse Test on S. aureus

10/18/2016
Organisms that have the catalase enzyme can detoxify the cell of hydrogen peroxide by converting it to oxygen gas. Bubbles indicate oxygen gas production after the addition of hydrogen peroxide directly to colonies growing on the TSA plate.

Organism mix on a hand print

10/15/2016
Mix of organism seen on a handprint/finger on TSA after incubation at 37 degrees for 24 hrs then held at refrigerated temperatures for 1 month.

Happy Halloween MAC Style

10/07/2016
E. Coli grown on MacConkey Agar at 37 degrees for 24 hours in the shape of a jack-o-lantern face. -Important Ingredients: crystal violet, bile salts, neutral red (color indicator), lactose -Differential: used to identify and isolate lactose fermenting G- enteric bacteria mainly Enterobacteriaceae. Strong lactose fermentation produces pink colonies with bile salt ...

Happy Halloween!

10/07/2016
Staphylococcus aureus grown on Mannitol salt agar (MSA) for 24 hrs at 27 degree's C in the shape of a jack-o-latern smile. MSA is a selective and differential agar used to differentiate between pathogenic S. aureus and S. epidermidis •Important Ingredients: Phenol red, mannitol (sugar), 7.5% NaCl •Selective: NaCl inhibits non-halophilic organisms. Organism ...

Serratia marcesens on Casein

10/05/2016
Casein Plates contain the major milk protein found in milk. Serratia marcesens was grown on Casien agar for 72 hours at 37 degree's C to look for the presence of the exoenzyme proteases/caseases . Serratia marcesens exhibits casein hydrolysis as well as red colony pigment prodigiosin.

Pseudomonas

10/05/2016
Casein Plates contain the major milk protein found in milk. Pseudomonas aeruginosa grown on Casien agar for 72 hours at 37 degree's C to look for the presence of the exoenzyme proteases/caseases . Pseudomonas aeruginosa, casein hydrolysis, as noted by a zone of clearing around the organism, as ...

5% NaCl BHI Media Contminant

09/25/2016
Fungal contaminant, probable airborne, seen on BHI media containing 5% NaCl after 2 months at refrigerated temperatures. White hyphal "apron" with no spore formation can be seen at the edges with brown spore formation seen in the center.

Serratia marcescens on Mac Agar

08/18/2016
Serratia marcescens a Gram (-) rod seen on MaConkey Agar a selective differential agar used to isolate Gram (-) organisms. Culture was grown at 37 degree for 24 hrs, cultures we light pink. The plate was held at room temperature for 24 hrs after that and the majority ...

Microbiome: A Cultural Revolution

08/18/2016
The Microbiome is enjoying some much deserved attention as people are starting to realize that we are not alone in our body.....and its a good thing! Culture was done using a large TSA plate, Serratia marcescens (red), Staphylococcus epidermidis (white, arms and legs), and an unknown environmental yeast (yellow). ...

Table top Bacillus

08/15/2016
Swab taken from the lab table top and incubated on TSA for 48 hrs, left at room temperature for 72 hrs then held at refrigerated temperature for a month. Pictured is an unknown isolated colony, more than likely Bacillus spp, beige in color, dry mat with rhizoid growth in the ...

Table Top Swab III

08/10/2016
Swab taken from the lab table top and incubated on TSA for 48 hrs, left at room temperature for 72 hrs then held at refrigerated temperature for a month. Pictured is an unknown isolated beige, mucoid colony. The center was raised and if the plate was tiled it would ...

E. coli with pGFP expression

07/29/2016
Streak plate of E. Coli transformed with the plasmid Green Fluorescent Protein (pGFP). Under UV light the colonies expressing GPF fluoresce green, colonies with normal light were a light green as well. Culture was grown on LB agar with ampicillin for 48 hrs.

Unusual contaminant morphology

07/23/2016
Unknown contaminant seen growing on Brain Heart Infusion (BHI) agar plate after several months at refrigerated temperatures, The organism grew at the edge of the agar plate interface, was smooth and circular, cream/beige mat at the base which mounded up, the edges or center of the colony had finger/hair ...

Fungal contaminant on BEA

07/22/2016
Fungal contaminant seen on Bile Esculin Agar (BEA) after several months at refrigerated temperatures. The fungus was circular at the edges with mounding in the center and blackening of the media indicating esculin hydrolysis.

Table Top Color

07/22/2016
Swab taken from the lab table top and incubated on TSA for 48 hrs, left at room temperature for 72 hrs then held at refrigerated temperature for a month. Pictured is a colorful mucoid orange yeast (possible rhodotorula) slowly overtaking several other colonies (yellow is a presumable yeast as well). ...

Table top mix

07/22/2016
Swab taken from the lab table top and incubated on TSA for 48 hrs, left at room temperature for 72 hrs then held at refrigerated temperature for a month. Pictured is a colorful mix of beige mucoid bacteria, orange and yellow mucoid yeast/bacteria, and white grainy fungi (possible actinomyces?).

Unknown Environmental Swab from Handrail

07/21/2016
Out door wood railing was swabbed onto TSA and grown for 48 hrs at 37 degree's C, then allowed to grow at room temperature for 72 hrs the held at refrigerated temperature for a month. This is a close up of some of the organisms that grew. One was a ...

Table Top Swab II

07/21/2016
Swab taken from the lab table top and incubated on TSA for 48 hrs, left at room temperature for 72 hrs then held at refrigerated temperature for a month. Pictured is one of the resulting bacterial/fungal growths (yeast): Circular yellow colony, mucoid, with a donut like appearance

Yellowstone Caldera/Bacterial Mat

07/10/2016
Bacterial mats, golden brown in color, seen at the outer edges of a geothermal vent/geyser. Cyanobacteria grow around 163 F or lower. Yellowstone National Park.

Floor swab organism/Swarming

07/10/2016
Floor swab was done for a science project. One of the organisms seen was mucoid, opaque in color and had finger like projections (swarming) after growth on TSA at 37 degree's C for 48 hrs.

Serratia marcescens/

07/10/2016
Serratia marcescensgrown on TSA for 48 hrs. at 37 degree's C then held at room temp for 24 hrs. Serratia marcescens is circular, mucoid and has a red to oragne pigment due to the production of prodigiosin. Usually the red coloration dose best at reduced temperatures so ...

Mold contaminant on yogurt

07/05/2016
Mold culture growing on top of a home made yogurt culture. Yogurt was made using whole milk and incubated for 8 hrs at a ~30 degree's C then refrigerated. This culture had been in the refrigerator for over 2 weeks, well past expiration for home made yogurt. ...

Lichen Fruiting Bodies

07/01/2016
Lichen (Xanthoria spp) Fruiting bodies, disc like structures, seen on the shady side of tree in Santa Cruz, Ca.

Table Top swab

06/24/2016
Swab taken from the lab table top and incubated on TSA for 48 hrs, left at room temperature for 72 hrs then held at refrigerated temperature for a month. Pictured is one of the resulting fungal growths: white with no spore formation, droplets can be seen as possible ...

Unknown Environmental Swab

06/24/2016
Out door wood railing was swabbed onto TSA and grown for 48 hrs at 37 degree's C, then allowed to grow at room temperature for 72 hrs the held at refrigerated temperature for a month. This is a close up of some of the organisms that grew. One ...

Unknown contaminant on Blood agar close up #2

05/01/2016
Unknown contaminant, possible Bacillus spp, seen on an old blood agar plate that had been inoculated with Strep bovis. The plate had been incubated at 37 degree's C for 24 hrs then held at room temp for a week. One colony was mucoid with a brain like appearance the other ...

Unknown contaminant on Blood agar close up

05/01/2016
Unknown contaminant, possible Bacillus spp, seen on an old blood agar plate that had been inoculated with Strep bovis. The plate had been incubated at 37 degree's C for 24 hrs then held at room temp for a week. One colony was mucoid with a brain like appearance the other ...

Fungal contaminant

05/01/2016
Unknown fungal contaminant grown up the side of a TSA plate. Plate had been in the refrigerator for several weeks. Colony was round with hair like growths in the center.

Fungal contaminant

05/01/2016
Unknown fungal contaminant grown up the side of a TSA plate. Plate had been in the refrigerator for several weeks. Colony was round with hair like growths in the center.

Unknown contaminant on Blood agar

05/01/2016
Unknown contaminant, possible Bacillus spp, seen on an old blood agar plate that had been inoculated with Strep bovis. The plate had been incubated at 37 degree's C for 24 hrs then held at room temp for a week. One colony was mucoid with a brain like ...

Unknown organism/Palm of hand print #2

04/13/2016
Unknown organism, possible Bacillus spp, seen on finger of a hand print done on a 4th grade class. Organism was a mucoid beige/white color and rose up/mounded in the center. The edges were smooth with slight rhizoid like growth through out. TSA plates incubated at 37 degree's C for ...

Unknown on palm of hand print

04/13/2016
Unknown organism seen on the palm of a hand print. Organism was a light mat yellow color with a mounding/rhizoid growth. Organism grew up (3-D)c forming what looked like a basket. Plate were TSA incubated for 24 hrs at 37 degree's C, then 3 days at ...

Unknown Organism on handprint

04/13/2016
Unknown organism, possible Bacillus spp, seen on finger of a hand print done on a 4th grade class. TSA plates incubated at 37 degree's C for 24 hrs, left at room temp for 3 days then held at refrigerated temperatures.

Shelf Mushroom

03/19/2016
Shelf mushroom seen growing on the side of a tree in the Santa Cruz Mt's.

Droplets on Mold

03/13/2016
Droplets due to respiration or possible antibiotic production seen in the center of a mold colony. Mold grew pink in the center with green spore formation around the edges and a white non spore forming "apron" This is a fairly common environmental contaminant seen in our lab , ...

Center of fungal colony

03/06/2016
Close up of the center of an unknown mold colony seen on TSA, presumably an airborne contaminant. The center is raised and "fluffy" with spore formation seen around it.

Alcaligenes faecalis line inoculation with swarming

02/01/2016
A line inoculation was done with Alcaligenes faecalis and incubated for 48 hrs at 37 degree's C. Swarming/spreading could be seen form the inoculation line. Note: Small contaminate also seen on the plate.

Pseudomonas aeruginosa with pigment

02/01/2016
A line inoculation was done with Pseudomonas aeruginosa. After 48 hrs incubation at 37 degrees C incubation the blue/green diffusible pigment pyocanin could be seen around the organism. There was also the characteristic grape smell.

More Fungus

02/01/2016
After several weeks of heavy rains several different mushroom species were seen in the leaf litter and shade of a cedar tree in Santa Cruz, CA. The fungus was yellow/orange, undulating appearance, with what looked like rough bumps on the outer edges with the inside being smooth.

NY Subway Experiment/Craig Ward

01/23/2016
The hand print image was featured in a short video by Emily Driscoll for NPR, swabbing the NY subway by artist Craig Ward. Note: the only thing I contributed was the image. I was not involved in the experiment. I would not advocate for doing this experiment ...

Mushroom/Fungus #4

01/22/2016
After several weeks of heavy rains several different mushroom species were seen in the leaf litter and shade of a pine tree in Santa Cruz, CA. The fungus was yellow/orange two toned color, slimy, and had an undulating appearance.

Mushroom/Fungus #3

01/22/2016
After several weeks of heavy rains several different mushroom species were seen in the leaf litter and shade of a pine tree in Santa Cruz, CA. The cap was greenish yellow with some orange, had a dull rough texture, was cone shaped (others had opened up and flattened out a ...

Mushroom/Fungus #2

01/22/2016
After several weeks of heavy rains several different mushroom species were seen in the leaf litter and shade of a pine tree in Santa Cruz, CA. The cap was beige/tan, smooth and started to invert, gills were not attached to the stem.

Unknown organism/Palm of hand print #2

01/22/2016
Unknown organism, probable Bacillus spp, seen on palm of hand print done on MH, incubated at 37 degrees for 2 days, room temp for 3+ days then refrigerated temp for one month. Organism was beige, circular, mat towards the edges. The center was rhizoid/folded, hard to tell if there ...

Unknown organism/Palm of hand print

01/22/2016
Unknown organism, probable Bacillus spp, seen on palm of hand print done on MH, incubated at 37 degrees for 2 days, room temp for 3+ days then refrigerated temp for one month. Organism was beige and mucoid, smooth growth on one side and rhizoid growth throughout the rest of ...

Purple Mushroom/Fungus, underside

01/18/2016
Purple mushroom seen after several day of heavy rain in the leaf litter and shade of a pine tree. in Santa Cruz, CA. This mushrrom had been knocked over exposing the underside of the cap. The cap was smooth and almost mucoid, gills were not attached to the stem ...

Purple Mushroom/Fungus

01/18/2016
Purple mushroom seen after several day of heavy rain in the leaf litter and shade of a pine tree. in Santa Cruz, CA The cap was smooth and almost mucoid, gills were not attached to the stem

Cooties Catcher/Hand print

01/16/2016
I was asked to consult on a hand print experiment done in a public school in NY showing what grows on our hands and the importance of hand washing....did not know I was going to be in it. A nice start to finish hand print "experiment" in a short ...

Unknown Organism/hand print finger tip

12/30/2015
Unknown organisms seen on finger tip on MH. One organism is round, smooth and mucoid almost transparent the other is mat, with several rings of lumpy growth. The mucoid colony is starting to overgrow the other colony. Both are possible Bacillus spp.

Unknown Organism/hand print palm

12/30/2015
Organism was seen on the palm of a hand print done on MH. The large center portion of the colony, possible Bacillus, was round and mucoid, would drip if the plate was tilted. The organism spread from the center through rhizoid growth, what looks like a network of ...

Unknown Organism/hand print

12/30/2015
Unknown organism seen on the tip of a finger imprint done on MH. The colony, possible bacillus, was dry/mat throughout the colony. There were 3 pouches of liquid that can be seen in the colony. When the plate was tilted the liquid portion moved but did not ...

Microbiologist Christmas Wreath, Unknown Organisms seem on Washed Hand Print #2,

12/24/2015
Unknown organism seem after washing hands on a hand print grown on TSA. Larger organism (possible Bacillus spp) is circular, edges not smooth, mucoid with rhizoid like growth towards the edges. Second smaller organism is yellow, mucoid, and has something erupting from the center.

Unknown Organism on Washed Hand Print

12/22/2015
Unknown organism, possible Bacillus spp, washed hand print done on a TSA plate. Organism was the size of a dime, muciod/ rhizoid in the center and mat/smooth at the outer edges.

Unknown Contaminant on BEA

12/21/2015
Unknown organism, presumable yeast, seen on Bile Esculin Agar (BEA) after several months at refrigerated temperatures. Organism was mucoid.circular, and a light brown in color. The brown coloration around the colony indicates the organism can to convert the esculin into 6,7-dihydroxy-couarin producing the black pigment in the agar. ...

Enlarged view of winter hand print

12/20/2015
Enlarged view of cold wet (winter) hand print.

Enlarged view of summer hand print

12/20/2015
Enlarged view of dry warm (summer) hand print.

Sesonal Hand print

12/19/2015
Bacterial hand print done last week with a group of 4th graders (~30 plates done). This same thing was done 4 months ago with another group of 4th graders when the weather was warm and dry. A different hand "flora" can be seen between the two seasons. ...

Unknow FUngal contaminant on Lipid agar

12/18/2015
Unknown fungal contaminant seen on Lipid agar after several months at refrigerated temperatures. Image taken using transmitted light to highlight the concentric rings of the colony. COlor was a dark brownish green.

Bacillus spp

12/16/2015
Bacillus spp seen on the finger tip of a hand print grown on MH agar at 37 degree's C. Colony was the size of a dime with rhizoid branching growth.

Unknown Organis /hand print

12/16/2015
Unknown organism seen on a hand print done on MH agar incubated for 24 hrs at 37 degree's C. The edges of the colony were dry and mat but the center was mucoid/liquid. When the plate was tilted the liquid center of the colony look as if ...

Handprint Culture Shirt

12/10/2015
"Everyone Needs a Little Culture" shirt @ Cabrillo College bookstore, 831-479-6209 or Niles Biological, http://www.nilesbio.com/cat499.html

ASM Santa Hat/Happy Holidays

12/03/2015
ASM Santa Hat....Bacteria used: Red portion of hat and letters: Serratia marcescens White hat trim: Staph saprophyticus Large TSA plate grown for 24 hrs at 37 degree's C

Holiday Time, smile!

12/03/2015
Holiday time......Bacteria/Yeast used: Hat and letters, Serratia marcescens Hat trim: Staph epidermidis Smile: Environmental yeast isolate Mix of Serratia and Staph for a bit of "snow"

Nitrate reduction test

11/13/2015
Nitrate reduction test for the reduction of nitrate in nitrate media, to determine if the bacteria contains nitrate and/or nitrite reductase. Organism were incubated for 48 hr's at 37 degree's in nitrate media then reagents added. Slide (1) Nitrate broths after the addition of 10 drops reagents ...

Unknown Organism on Washed Hand Print

11/10/2015
Unknown organism, possible Bacillus spp, seen on a hand print after hand washing. TSA plate was grown at 37 C for 24 hours then left at refrigerated temps for 4 weeks. Organism was a light brown and mat. The center of the growth was smooth with rhizoid like growth ...

Unknown Organism seem on Washed Hand Print

11/10/2015
Unknown organism, possible yeast or Bacillus, seen on a hand print after hand washing. TSA plate was grown at 37 C for 24 hours then left at refrigerated temps for 4 weeks. Organism was light yellow and mucoid . There are numerous rings of growth (looks like layers of rope) ...

Unknown Organism Washed Hand

11/10/2015
Unknown organism, possible yeast, seen on a hand print after hand washing. TSA plate was grown at 37 C for 24 hours then left at refrigerated temps for 4 weeks. Organism was light yellow, mat in the center and mucoid towards the outside. There are several rings ...

After Hand Washing

10/28/2015
This organism seems to be showing up after students wash with soap and water. Organism seen on finger tip grown on TSA 37 degree's C for 48 hrs then refrigerated temp for several weeks. Organism was a pale yellow with raised rhizoid growth in the center some of ...

Interesting Colonoy Morphology/Formation

10/28/2015
Hand print done on a large TSA plate after using hand sanitizer, this was the only colony seen. Large cream/white matt colony covering the majority of the plate. The center of the colony was solid with speckled growth/offshoots at the edges, possible Bacillus spp.

Unknown Organism from Environmental Swab

10/28/2015
Unknown organism from an environmental swab, swab was done on the bottom of a students backpack. Organism was grown on TSA at 37 degree's C for 48 hours then refrigerated for several weeks. Color was a white with a mattt apperance in the middle with lobate mucoid margins. ...

Unknown Organism on Hand Print #2

10/20/2015
Unknown organism seen on a hand print after washing with soap and water grown on a TSA plate for 24 hrs, room temperature for a day and several days at refrigerated temp. Organism was a pale yellow with raised rhizoid growth in the center some of it matt and some ...

Grape Leaf

10/18/2015
A small grape leaf was pressed/printed onto a large TSA plate incubated for 48 hrs at 37 degree's C, then place at refirigerated temps for 3 days. The result was a mix of yeast (yellow/orange) , several Bacillus spp and a variety of other unknown organisms. The outline ...

Unknown Organism on handprint

10/18/2015
Unknown organism seen on a hand print after washing with soap and water grown on a TSA plate for 24 hrs, room temperature for a day and several days at refrigerated temp. Organism was a mat pale yellow with raised rhizoid growth in the center and smooth edges.

Unknown black puffball

10/05/2015
Unknown black puffball seen growing on the bark of a fallen oak tree in the Santa Cruz Mt's, CA. There were numerous puffball all about the size of a dime.

Unknown Fungal contaminant on Mueller Hinton agar #2

10/01/2015
Unknown fungal contaminant seen on Mueller Hinton agar after several months at refrigerated temperature. Colony had a white non-spore forming apron around the edges with green spore formation seen on the inner layer/center.

Unknown Fungal contaminant on Mueller Hinton agar

10/01/2015
Unknown fungal contaminant seen on Mueller Hinton agar after several months at refrigerated temperature. Colony had a white non-spore forming apron around the edges with green spore formation seen on the inner layer. The center was raised and droplets can be seen as a result of ...

Unknown Bacillus #2

09/26/2015
Students swabbed the lab bench at the end of class. The TSA plate was grown for 48 hrs at 37 degrees C then let set for 2 days at room temperature. The close up of one of the colonies was white/matt through out, with rhizoid/undulating growth. This is more than ...

Unknown Bacillus

09/26/2015
Students swabbed the lab bench at the end of class. The TSA plate was grown for 48 hrs at 37 degrees C then let set for 2 days at room temperature. The close up of one of the colonies was yellow/mucoid through out, with rhizoid growth. This is more ...

Unknown Bacillus spp Overgrowing another colony

09/20/2015
Unknown Bacillus spp grown on TSA, seen on the tip of a ring finger imprint, overgrowing another unknown colony.

Fungal Contaminant on 5% NaCl BHI

09/20/2015
White fungal contaminant on 5% NaCl BHI after several months at refrigerated temperatures. White hypal formation was seen with no spore formation.

Thumb print/Bacillus spp

09/20/2015
Unknown Bacillus spp. seen on the tip of a thumb print from a 1st grader. A large TSA plate was used and culture was incubated for 24 hrs at 37 degree's C.

Unknown White Fungal contaminant on 7.5% NaCl BHI #2 different view

09/13/2015
Different view of Unknown Fungal contaminant on 7.5% NaCl BHI after several month at refrigerated temperatures. White hyphae was seen spreading into the agar with no spore formation.

Unknown White Fungal contaminant on 7.5% NaCl BHI #2

09/13/2015
Unknown Fungal contaminant on 7.5% NaCl BHI after several month at refrigerated temperatures. White hyphae was seen spreading into the agar with no spore formation.

Unknown Fungal contaminant on 7.5% NaCl BHI

09/13/2015
Unknown fungal contaminant, presumably airborne, found on BHI containing 7.5% NaCl after several months at refrigerated temperatures. Concentric rings were seen with brown spore formation seen in the middle ring of the colony with hyphae at the edges and center of the colony. Colony covered the entire plate.

Unknown Fungal contaminant on 7.5% NaCl BHI

09/13/2015
Unknown fungal contaminant, presumably airborne, found on BHI containing 7.5% NaCl after several months at refrigerated temperatures. Concentric rings were seen with brown spore formation seen in the middle ring of the colony with with hyphae at the edges and center of the colony. Colony covered the entire plate.

Unknown Contaminant on BHI

09/13/2015
Unknown contaminant see on BHI after several months at refrigerated temperatures. Organism was a matte pale beige with mounding in the center and concentric rings on the outer edges. Gas bubbles were seen in the media on the outer edges of the colony growth.

Hulk Hand

09/05/2015
Bacterial Hand Print doen on TSA agar, incubated for 48 hrs at 37 degree's C. Bacteria seen are mainly Bacillus spp.

Interesting morphology on finger tip

08/30/2015
Close up of an unwashed finger, print done by a student. The student later informed me that part of his job in the morning was feeding chickens on the farm.

Colorful cluster of Bacteria

08/30/2015
Colorful cluster of bacteria seen on a palm print. Possible Bacillus, Staph, Micrococcus, Serratia and yeast.

Neisseria sicca on blood agar

08/25/2015
Neisseria sicca grown on blood agar at 37 degrees C for 5 days. A Gram (-) diplococcus organism that is part of the normal mucosal membranes and does not normally cause disease.

Unknown Yeast/Bacteria

07/03/2015
Unknown Yeast or Bacteria seen on tip of finger print grown for 2 days at 37 degrees C then held at room temperature for 3 days. Organism was yellow, mucoid, raised rhizoid with large holes.

Unknown Yeast/Bacteria

07/03/2015
Unknown Yeast or Bacteria seen on palm print grown for 2 days at 37 degrees C then held at room temperature for 3 days. Organism was yellow, mucoid, raised (piled) rhizoid.

Unknown Bacillus

07/03/2015
Unknown Bacillus from handprint. Colony was large, almost half the plate, and exhibited rhizoid growth. Plate was grown at 37 degree C for 2 days then held at room temp for 3+ days.

Unwashed vs washed hand

06/29/2015
Students in the summer STEM program at Cabrillo (these were college age students) were asked to do a handprint of their unwashed hand then of their hand after washing. This is a before and after of what the majority of the plates looked like. Plates were ...

Unknown isolate from table swab

06/20/2015
Students swabbed the lab bench at the end of class. The TSA plate was grown for 48 hrs at 37 degrees C then let set for 2 days at room temperature. The close up of one of the colonies was matte/white through out, a smooth center ...

"E. coli Happens"

06/14/2015
E. coli was grown on MacConkey Agar (MAC) at 37 degrees for 24 hour. MAC is a Selective and Differential media used to inhibit G+ growth and some G- bacteria as well as identify and isolate lactose fermenting G- enteric bacteria mainly Enterobacteriaceae. Strong lactose fermentation produces pink colonies ...

Unknown from Handprint #3

06/04/2015
Unknown organism seen on a TSA plate used to do a handprint. Organism was seen on the outside of the imprint so not sure if it came from the hand or was contaminant. Colony was circular, tan/white, rhizoid growth in the center with a smooth margin and mucoid ...

Unknown from Handprint #2

06/04/2015
Closeup of organism seen on handprint, presumably Bacillus sp. Organism grew out from the center with rhizoid growth, lobate edges, tan coloration, glossy throughout the colony,

Unknown from Handprint #1

06/04/2015
Organism seen on handprint, presumably Bacillus sp. Organism grew out from the center with rhizoid growth, lobate edges, tan coloration, glossy throughout the colony,

Hand print

06/04/2015
Hand print on a large TSA plate from my 8 1/2 year old son after playing outside.

Unknown Red/Pink Yeast

05/29/2015
Unknown yeast, possible Rhodotorula sp., isolated on TSA from a swab taken from the bottom of a shoe incubated at 25 and 37 degrees C. Red/Pink, highly mucoid colonies were seen after 2 weeks at 25 degrees (image) and pin point colonies seen at 37 degrees C after 2 ...

Unknown Bacillus

05/28/2015
Unknown Bacillus isolated on TSA from a swab taken from the bottom of a shoe. Organism was incubated at both 25 and 37 degrees C with growth on the 37 degree plate after 1 week. No growth was seen on the 25 degree plate. Colonies were orangish/yellow, ...

Yellow Unknown Yeast

05/28/2015
Unknown yeast isolated on TSA from a swab taken from the bottom of a shoe incubated at 25 degrees C. Yellow, mucoid, round colonies were seen after 2 weeks. This isolate grew equally well at 37 degree C with a growth time of ~ 1 week.

Tree Fungus

05/25/2015
Close up of an unknown tree fungus seen growing on a red wood tree (living) in Davenport, CA. Gills were free and not attached to a stem and the cap turned upwards.

Possible Slime mold

05/25/2015
Image of a Slime Mold taken at Hoh Rainforest on the Olympic Peninsula in Western Washington, largest Temperate Rain forest in the United States. Several patches, white and mucoid, were seen on the underside of a fallen redwood tree.

Unknown Agar Contaminat

05/25/2015
Unknown contaminant seen on TSA agar after several months at refrigerated temperatures. Image was taken using transmitted light which highlights the concentric rings.

Unknown Fungal Contaminant

05/25/2015
Unknown fungal contaminant, presumably airborne, found on TSA after several months at refrigerated temperatures. Brown spore formation was seen in the middle of the colony with whit hyaphae at the edges. Colony was approximatly 1/4 of the plate.

Orange Fungus image response

05/19/2015
Image taken in Jamestown in March, similar to previous post. Several people in our dept have suggested chechen of the forest or SIlvershelf.

Unknown environmental contaminant on blood agar plate streaked with Enterococcus faecalis

05/15/2015
Unknown environmental contaminant on blood agar plate streaked with Enterococcus faecalis. Plated was incubated at 37 degrees C for 24 hrs then held at room temperature. Contaminant was not seen until plate was held at room temp for several weeks. The organism was red, mucoid, and ...

Unknown Yeast Contaminant

05/15/2015
Unknown Yeast contaminant seen on Casein media. The contaminant was around the edges of the plate with isolated colonies seen towards the center of the plate after 1 month refrigerated temperatures. Colonies were light opaque pink, mucoid and had concentric rings slightly elevated in the center. ...

Fungal contaminant on Casein media

05/06/2015
Unknown fungal contaminant seen on Casein media after 1 month at refrigerated temperatures. Green spore formation is seen throughout the colony with white hyphal growth on the outer edge of the colony. The center was raised and had deep groves.

Bile Esculin Agar

05/05/2015
Contains: bile the carbohydrate esculin iron salts Differential media: -Group D Streptococcus sp. will convert esculin into 6,7-dihydroxy-coumarin which reacts with the iron salts to form a black precipitate. --Non Group D Streptococcus sp. do not produce the black color. (A) Enterococcus faecalis (B) Streptococcus mitis (C) Streptococcus pyogenes (D) Streptococcus bovis

Fungus/Puff ball

05/02/2015
Image of a Puff ball taken at Hoh Rainforest on the Olympic Peninsula in Western Washington, largest Temperate Rain forest in the United States. The large white ball is the fruiting body which contains the spores.

Agar Contaminant presumably Yeast

05/02/2015
Agar contaminant seen on TSA after 1 month at refrigerated temperatures. Colony was yellow, mucoid, partially circular with concentric circular raised rings with an orangish center.

Proteus vulgaris/Swarming

05/02/2015
Streak plate of Proteus vulgaris. The plate was incubated at 37 degree C for 48 hrs then held at room temperature for 1 week. Swarming, white growth with finger like projections at the outer edges, around the circular solated colonies, circular white/beige opaque colonies, was not seen ...

Yeast contaminant

04/24/2015
Unknown contaminant, possible Yeast, seen on TSA agar after 3 weeks refrigerated temperatures. The colony appeared mucoid colony with lobate edges, was white in the center of the colony and yellow on the edges. The agar surrounding the colony had what appeared to be a brown coloration, ...

Fungal contaminant/Starch agar

04/08/2015
Fungal contaminant seen on Starch agar after 1 month at refrigerated temperatures. Colonies were blackish green and circular. Elevated tufts could be seen in the middle of each colony with small water droplets in the rhizoid growth.

Enterococcus faecalis

04/04/2015
Enterococcus faecalis grown on blood agar for 48 hrs at 37 degree's C. Small, pin point, circular colonies can be seen with gamma hemolysis (no hemolysis of the blood agar). Colonies were white with a glossy translucent appearance.

Klebsiella pneumoniae grown on Mac

04/02/2015
Klebsiella pneumoniae grown on Mac for 48 hrs at 37 degree's C. Pink, indicating lactose fermentation, mucoid colonies are seen.

Fungal Contaminant

03/31/2015
Fungal contaminant on TSA grown at refrigerated temperatures for 1 month. Colonies appeared rhizoid throughout with a fluffy appearance.

Unknown Fungus with spores different view

03/31/2015
Different view of TSA plate with fungal contaminant, presumably from the air, after 1 month at refrigerated temperatures. White center spore formation can be seen with white hypae on the edges. Spore formation can be see forming towards the edges.

Unknown Fungus with spores

03/31/2015
Unknown fungal contaminant on TSA after growth at refrigerated temperatures for 1 month. White hypae can be seen at the edges with green spore formation starting in the center.

Klebsiella pneumoniae on blood agar

03/29/2015
Klebsiella pneumoniae grown on blood agar for 48 hrs at 37 degree's C.

Fungal close up

02/20/2015
Close up image of the edge of an unknown fungal contaminant showing concentric rings of black spore formation (center of colony) and nonspore forming white hypae on the outer edge of the colony. Fungal contaminant was found on TSA after a month of refrigerated temperatures, presumably an air borne ...

Fungal contaminant close up

02/01/2015
Fungal contaminant, presumably airborne, seen on a TSA plate after 1 month at refrigerated temperatures. Close up image highlights exudate, water droplets due to respiration.

Fungal Contaminant

02/01/2015
Fungal contaminant, presumably airborne, seen on a TSA plate after 1 month at refrigerated temperatures. White hyphal growth and exudate, water droplets due to respiration, can be seen through the colony.

Shoe Swab

02/01/2015
Unknown organism isolated from a swab taken off the bottom of a shoe. Students were asked to swab something in the classroom. One swab was done on the bottom of a shoe and incubated on a TSA plate for 48 hrs at 37 degree’s C. The organism was ...

Bed bug bite pattern

01/27/2015
Typical bed bug bite pattern, 3-5 bites clustered in a zigzag pattern also know as the breakfast, lunch, dinner pattern. Image taken 5 days after exposure.

Acid Fast Stain#2

01/24/2015
Acid Fast stain done on a mix of Staphylococcus aureus and Mycobacterium smegmatis. The stain was done on a culture of M. smegmatis that was incubated on a TSA slant with 1ml TSB added at 37 degree's C for 4 days, S. aureus was incubated in TSB at 37 ...

Bacillus/Diplobacillus

01/21/2015
Simple stain done on an unknown bacteria, showing feathery rhizoid growth on TSA after a 48 hr incubation at 37 degree’s C, isolated from a floor swab. Single bacillus and diplobacillus can be seen though out.

E. coli gram stain

01/20/2015
Gram stain of E. coli showing characteristic G- rods.

Unknown Fungal Contaminant

01/18/2015
Unknown Fungal Contaminant found on TSA plate that had been refrigerated for 1 month. Colony was raised in the center with deep groves around the colony. Green spore formation was seen in the middle with white non-spore forming hyphae towards the edges.

Aquaspirillum serpens

01/18/2015
A simple stain done on a stock culture of Aquaspirillum serpens to look at cell shape. This culture was grown in TSB at room temperature, ~21 degrees C, for several months. Aquaspirillum is a slow grower normally found in pond water. A characteristic spiral rod ...

Swarming Motility/ Proteus mirabilis

01/08/2015
A sample of Proteus mirabilis was inoculated on to a TSA plate by taking a sterile swab and placing a drop in the center of the plate. The plate was then incubated at 37 degrees for 8 hrs. Swarming motility can be seen as a clear halo and ...

Unknown environmental Contaminant on blood agar plate streaked with S. pyogenes

01/08/2015
A streak plate of Strep pyogenes on blood agar, stock culture used for class, was kept at room temp for 1 month. Before disposal a contaminant, presumably environmental, was seen on the plate. The pigment was opaque tan/yellow and with a mucoid surface. Lobate marigins with raised irregular-rhizoid ...

Happy Holidays!

12/03/2014
Large TSA plate: yellowish organism is Staph aureus, red organism is Serratia marcescens. Grown for 24 hrs at 37 degrees C.

Unknown Fungal Contaminant/MAC

11/15/2014
Unknown fungal isolated contaminant found on MAC. MAC plate was incubated for 2 months at 4 degrees C once fungal growth was seen. This colony seemed to emerge from the agar and had a 3D appearance. The center of the colony had what seemed to be hyphal growth ...

Unknown Fungal Contaminant/TSA #2

11/15/2014
Two unknown airborne fungal isolated contaminant found on TSA. TSA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Concentric rings of color (pink/peach, green and white) can be seen throughout the colonies.

Unknown Fungal Contaminant/TSA

11/15/2014
Unknown airborne fungal isolated contaminant found on TSA. TSA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Concentric rings of color (pink, green and white) can be seen throughout the colony with smaller colonies seen around the white edges of the colony due ...

Unknown Fungal Contaminant/View 2

11/10/2014
Unknown airborne fungal isolated contaminant found on BEA. BEA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Spore formation can be seen throughout the circular colony. The single colony covered ¼ of the plate. Image highlights the center growth ...

Unknown Fungal Contaminant/View 1

11/10/2014
Unknown airborne fungal isolated contaminant found on BEA. BEA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Spore formation can be seen throughout the circular colony. The single colony covered ¼ of the plate.

Unknown Fungal Contaminant

11/10/2014
Unknown fungal contaminant found on TSA. TSA plate was incubated at 4 degrees for two months after contaminant was discovered.

Airborne Environmental Isolate/Fungal view 2

10/18/2014
Different view of an unknown airborne environmental isolate on Bile Esculin Agar (BEA) exhibiting a single circular colony. White hyphal growth with no spore formation. Clear to brownish exudate can be seen throughout the colony. This sample grew at refrigerated temperatures for several months. The agar surrounding the ...

Airborne Environmental Isolate/Fungal view 1

10/18/2014
An unknown airborne environmental isolate on Bile Esculin Agar (BEA) exhibiting a single circular colony. White hyphal growth with no spore formation. Clear to brownish exudate can be seen throughout the colony. This sample grew at refrigerated temperatures for several months. The agar surrounding the colony did turn ...

Gelatin Hydrolysis

10/16/2014
Pseudomonas aeruginosa, Escherichia coli and Serratia marcescens were inoculated into gelatin agar using the stab method and incubated for 1 week at 37 degree’s C. If the organism has gelatinase then it can break down the gelatin (protein) into polypeptides and then amino acids which can then be ...

Temperature

09/28/2014
(A) Staphlococcus aureus, (B) Bacillus stearothermophilus, (C) Pseudomonas fluorescens and (D) Escherichia coli were grown on TSA for 24 hrs at varying incubator temperatures (4, 25, 40, and 60 degree’s C). Note: The 4 degree plate were incubated for a week to get good Pseudomonas fluorescens growth.

Osmotic pressure

09/28/2014
(A) Bacillus subtilis, (B) Staphlococcus aureus and (C) Escherichia coli were grown on BHI media with varying concentrations of NaCl (0.85%, 5%, 7.5%, 10%, and 20%) at 37 degrees C for 24 hrs.

Osmotic pressure

09/28/2014
A) Bacillus subtilis, (B) Staphlococcus aureus and (C) Escherichia coli were grown on BHI media with varying concentrations of NaCl (0.85%, 5%, 7.5%, 10%, and 20%) at 37 degrees C for 24 hrs.

Osmotic pressure

09/28/2014
A) Bacillus subtilis, (B) Staphlococcus aureus and (C) Escherichia coli were grown on BHI media with varying concentrations of NaCl (0.85%, 5%, 7.5%, 10%, and 20%) at 37 degrees C for 24 hrs.

6.5% NaCl Media

08/15/2014
Important ingredients: 6.5% salt, dextrose, bromcresol purple Differential/Selective: used to test for salt tolerance and can be used to identify enterococcal group D streptococci. NaCl is a selective agent and those organisms that can grow in higher salt concentrations also ferment the dextrose in the media. This causes acid ...

Methyl Red/Vogoes-Proskauer (MR-VP)

07/20/2014
Methyl Red/Vogoes-Proskauer (MR-VP) One medium used for two separate but related tests, identifying butanediol (VP) or acidic fermentation products (MR) as part of the IMViC test used in the identification of Enterobacteriaceae. •Methyl Red (MR): detects the ability of an organism to produce acidic fermentation products. Acidic procucts produce a red ...

SIM Media

07/13/2014
Sulfur-indole-motility test (SIM media) results for: (A) Escherichia coli: Motile***, hydrogen sulfide (-), indole (+)* (B) Staphylococcus aureus: Non-motile, hydrogen sulfide (-), indole (-) (C) Salmonella arizonae: Motile, hydrogen sulfide (+)**, indole (-) (D) Enterobacter aerogenes: Motile, hydrogen sulfide (-), indole (-) (E) Proteus vulgaris: Motile, hydrogen sulfide(+), indole (+) *After addition of 5-10 drops ...

Bacillus subtilis

07/04/2014
Gram stain of B. subtilis showing characteristic G +streptobacillus morphology.

Streptococcus pyogenes Gram Stain

06/27/2014
Gram stain done on S. pyogenes showing the characteristic long G+ streptococcus chains. Culture grown on blood agar for 48 hrs at 37 degree’s C.

Mycobacterium smegmatis Acid Fast Stain

06/27/2014
Acid Fast stain done on a mix of Staphylococcus aureus and Mycobacterium smegmatis. Carbol fuchsin was applied to the smear and set over a steaming water bath for 10 min to help penetrate the mycolic acid in the cell wall, then rinsed with acid alcohol which washes the ...

Endospore Stain Bacillus cereus

06/26/2014
B. cereus was grown on urea agar for 6 days at 37 degrees C to induce spore formation. The endospore stain was done using malachite green as the primary stain and safranin as counter the stain. Malachite green was applied to the smear and set over a steaming water bath ...

Starch Agar

06/18/2014
Starch agar contains high molecular weight starch molecules (soluable starch). When Iodine is added it reacts with starch to produces a dark brown/purple color. A zone of clearing forms around organisms that produce the exoenzyme amylase after addition of iodine. This plate was incubated for 24 hrs ...

Fluid Thioglycollate Media

06/03/2014
Organisms grown in Fluid Thioglycollate Media w/out resazurin dye is a reducing media that uses sodium thioglycollate to chemically combine with dissolved oxygen to deplete the oxygen in the media. All organism except for Neisseria sicca were grown for 48 hours at 37 degrees C. Neisseria sicca is a slow ...

Enterobacter aerogenes on MacConkey Agar (Mac)

05/24/2014
Streak plate isolation of Enterobacter aerogenes grown on MacConkey agar for 24 hours at 37 degree's. Colonies grow pink due to fermentation of lactose.

MaConkey Agar (Mac)

05/24/2014
Organisms grown on MacConkey Agar at 37 degrees for 24 hours. -Important Ingredients: crystal violet, bile salts, neutral red (color indicator), lactose -Differential: used to identify and isolate lactose fermenting G- enteric bacteria mainly Enterobacteriaceae. Strong lactose fermentation produces pink colonies with bile salt precipitation (hot pink). Weak fermentation produces ...

E.coli on MacConkey Agar (Mac)

05/23/2014
Streak plate isolation of E. coli on MacConkey Agar grown for 24 hrs at 37 degrees. E. coli demonstrates strong lactose fermentation indicated by the bright pink halo, bile precipitant around the colonies, and pink colony growth.

Unknown Agar Contaminate

05/18/2014
Unknown contaminant found on a TSA plate after being in the refrigerator for 1 month. Organism had grown throughout the media, was pale yellow and mucoid.

Unknown Contaminante

05/18/2014
Unknown contaminant found on a TSA plate after being in the refrigerator for 1 month. Organism had grow throughout the media.

Mannitol Salt Agar (MSA)

05/18/2014
Mannitol Salt Agar (MSA) •Important Ingredients: Phenol red, mannitol (sugar), 7.5% NaCl •Differential: between pathogenic S. aureus and S. epidermidis •Selective: NaCl inhibits non-halophilic organisms. Organism that can grow are halophilic. •Mannitol Fermentors: fermentation of acid causes a drop in pH of the media and the indicator (phenol red) turns yellow. A). ...

Mannitol Salt Agar (MSA)

05/18/2014
Mannitol Salt Agar (MSA) •Important Ingredients: Phenol red, mannitol (sugar), 7.5% NaCl •Differential: between pathogenic S. aureus and S. epidermidis •Selective: NaCl inhibits non-halophilic organisms. Organism that can grow are halophilic. •Mannitol Fermentors: fermentation of acid causes a drop in pH of the media and the indicator (phenol red) turns yellow. A). ...

Bacitracin test on Streptococcus pyogenes

05/15/2014
Bacitracin test done on a lawn of Streptococcus pyogenes grown on blood agar. The zone of inhibition around the bacitracin disc, approx 14mm measuring the entire length of the zone, indicates sensitivity. The zone of inhibition is red because the red blood cells did not lyse. ...

Streptococcus mitis on blood agar

05/15/2014
Growth of Streptococcus mitis on blood agar demonstrating alpha hemolysis seen as a greenish color around the growing colonies due to a reduction of the hemoglobin to methemoglobin in the surrounding agar. Image taken using transmitted light.

Mycobacterium smegmatis using transmitted light

05/07/2014
Isolated colony of Mycobacterium smegmatis grown on TSA for 96 hrs at 37 degrees C. Margin is lobate with an irregular-rhizoid form. Image taken using transmitted light.

Streptococcus pyogenes on blood agar

05/06/2014
Streak plate of Streptococcus pyogenes (incubated @ 37 degrees C for 48 hrs) showing beta hemolysis, complete lysis of red blood cells, around the isolated colonies. Image taken using transmitted light.

DNase Agar

04/29/2014
DNase agar can be used to differentiate between potentially pathogenic S. aureus and other Staphlococcus by looking for the presense of the exoenzyeme DNase. After growth for 24-48 hrs 1N HCL is added to the agar plate. Addition of HCL precipitates DNA in the media causing cloudiness ...

Kirby-Bauer disk diffusion

04/28/2014
Kirby-Bauer disk diffusion susceptibility test on coagulase-negative Staphylococcus aureus and E.coli grown on Mueller-Hinton agar with tetracycline (30 µg), cephalothin (30 µg), erythromycin (15 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), streptomycin (10 µg), and novobiocin (30 µg). Staphylococcus aureus and E.coli were chosen to ...

Lipid Agar

04/28/2014
Lipid Plate/Tributyrin Agar used to test for an organisms ability to produce the exoenzyme lipase which breaks down the lipids in the agar creating a clear zone around the organism. (A) Serratia marcesens, lipid hydrolysis, indicated by a zone of clearing around the growing colony, as well as the red ...

Casein Media

04/24/2014
Casein Plates contain the major milk protein found in milk. Organisms were grown on Casien agar for 72 hours at 37o C to look for the presence of the exoenzyme proteases/caseases . (A): Pseudomonas aeruginosa, casein hydrolysis, as noted by a zone of clearing around the organism, as ...

Micrococcus luteus line inoculation

04/23/2014
Line inoculation of Micrococcus luteus on a TSA slant showing Filiform, uniform growth, along the margin. Culture was grown for 3 days at 37 degrees, M. luteus usually takes 3+ days for good growth.

Mycobacterium smegmatis line inoculation

04/19/2014
Line inoculation of Mycobacterium smegmatis on a TSA slant showing friable, dry crusty, growth. Culture was grown for 3 days at 37 degrees.

Yellowstone Caldera, Wyoming

04/15/2014
Bacterial mats, golden brown in color, seen at the outer edges of Chromatic Pools. The blue water in the center is the hottest water at ~199 F and cooling to ambient the farther you get from the center. Cyanobacteria grow around 163 F or lower.

Bacterial Mat

04/14/2014
Yellowstone caldera, Wyoming. Golden brown thermophilic cyano-bacteria seen in the waters surrounding one of the hydrothermal vents.

Alcaligenes faecalis

04/13/2014
Streak plate of Alcaligenes faecalis grown on TSA for 48 hr.

Physarum polycephalum Different view

04/09/2014
Physarum polycephalum, slime mold, grown in a large perti plate on moist paper towels using oatmeal as the food source. Culture was grown in the dark at room temperature. The paper towel was moistened every day with tap water. After 3 week’s the culture formed sporangia (fruiting bodies). Image was ...

Tube morphology

04/06/2014
Three organisms inoculated in TSB to look at tube morphology. From left to right: Staphylococcus aureus: flocculent growth/turbid, growth throughout the tube. Mycobacterium smegmatis: pellicle, growth at the top of the tube. M. smegmatis tends to stick to the tube and grow up the side. Bacillus subtilus: sediment, ...

Blood agar/Hemolysis

04/06/2014
Three organisms inoculated onto blood agar, straight line inoculation, to demonstrate hemolysis. From top to bottom: Streptococcus pyogenes: beta, complete lysis of red blood cells, clear area around colony growth. Streptococcus bovis: alpha, incomplete lysis of red blood cells, green area around colony growth. Enterococcus faecalis: gamma, growth ...

Mycobacterium smegmatis

04/05/2014
Streak plate of Mycobacterium smegmatis grown on TSA for 96 hrs.

M. luteus/E.coli

04/05/2014
Streak plate isolation of Micrococcus and E. coli mixed culture.

Bacillus subtilis

03/30/2014
Streak plate isolation showing colonial morphology of Bacillus subtilis on trypticase soy agar (TSA) incubated for 24 hours at 37oC. Note the rough and dry/matte texture characteristic of this organism’s growth.

Unknown Floor isolate #2

03/30/2014
Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and mounding in an irregular-rhizoid form with lobate margins. The colony exhibited an opaque yellow-orange pigment with a mucoid surface. This sample was obtained from a floor swab and incubated at 37°C.

Unknown floor isolate

03/30/2014
Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and an irregular-rhizoid form with lobate margins. The pigment is opaque white-beige with a mucoid surface over most of the colony. A dry, dull, matte surface appears toward the outer edges of the colony. The ...

Environmental Swab/Bottom of Shoe #2

03/26/2014
Close up view of the edges of an unknown bacteria swabbed form the bottom of a shoe, possible Bacillus, on TSA exhibiting spreading and rhizoid growth. The pigment is opaque white-beige with a mucoid surface towards the center of the colony and a matte surface towards the edges. ...

Environmental Swab/Bottom of Shoe #1

03/26/2014
On the first day of Micro class instructors have students do an environmental swab. This sample was subcultured from a student who did the bottom of their shoe. The organism, possible Bacillus sp., was tough to scrap off the agar so subculture was done by taking a ...

Airborne Environmental Isolate #3

03/26/2014
An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the red/pink grainy spore formation in the center of the colony. This sample grew at refrigerated temperatures for ...

Airborne Environmental Isolate #2

03/26/2014
An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the green and yellow grainy spore formation in the center of the colony with exudate in the center. ...

Airborne Environmental Isolate

03/26/2014
Media is poured in bulk in our lab for classroom use. Although contamination is low we do see airborne contamination especially durning the rainy season. This is an unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth can be seen throughout the ...

Mycobacterium smegmatis

03/24/2014
Mycobacterium smegmatis grown on TSA for 96 hrs.

Mycobacterium smegmatis

03/24/2014
Mycobacterium smegmatis grown on TSA for 96 hrs.

Serratia marcescens/Escherichia coli mix

03/24/2014
Serratia marcescens/Escherichia coli mix grown on TSA for 48 hrs. Serratia marcescens grows red due to prodigiosin and Escherichia coli grows opaque white.

Micrococcus luteus

03/24/2014
Micrococcus luteus grown on TSA for 96 hrs.

Physarum polycephalum

03/21/2014
Physarum polycephalum, slime mold, grown in a large perti plate on moist paper towels using oatmeal as the food source. Culture was grown in the dark at room temperature. The paper towel was moistened every day with tap water. After 3 week’s the culture formed sporangia (fruiting ...
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