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Happy Holidays!

12/03/2014
Large TSA plate: yellowish organism is Staph aureus, red organism is Serratia marcescens. Grown for 24 hrs at 37 degrees C.

Unknown Fungal Contaminant/MAC

11/15/2014
Unknown fungal isolated contaminant found on MAC. MAC plate was incubated for 2 months at 4 degrees C once fungal growth was seen. This colony seemed to emerge from the agar and had a 3D appearance. The center of the colony had what seemed to be hyphal growth ...

Unknown Fungal Contaminant/TSA #2

11/15/2014
Two unknown airborne fungal isolated contaminant found on TSA. TSA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Concentric rings of color (pink/peach, green and white) can be seen throughout the colonies.

Unknown Fungal Contaminant/TSA

11/15/2014
Unknown airborne fungal isolated contaminant found on TSA. TSA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Concentric rings of color (pink, green and white) can be seen throughout the colony with smaller colonies seen around the white edges of the colony due ...

Unknown Fungal Contaminant/View 2

11/10/2014
Unknown airborne fungal isolated contaminant found on BEA. BEA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Spore formation can be seen throughout the circular colony. The single colony covered ¼ of the plate. Image highlights the center growth ...

Unknown Fungal Contaminant/View 1

11/10/2014
Unknown airborne fungal isolated contaminant found on BEA. BEA plate was incubated for 2 months at 4 degrees C once fungal growth was seen. Spore formation can be seen throughout the circular colony. The single colony covered ¼ of the plate.

Unknown Fungal Contaminant

11/10/2014
Unknown fungal contaminant found on TSA. TSA plate was incubated at 4 degrees for two months after contaminant was discovered.

Airborne Environmental Isolate/Fungal view 2

10/18/2014
Different view of an unknown airborne environmental isolate on Bile Esculin Agar (BEA) exhibiting a single circular colony. White hyphal growth with no spore formation. Clear to brownish exudate can be seen throughout the colony. This sample grew at refrigerated temperatures for several months. The agar surrounding the ...

Airborne Environmental Isolate/Fungal view 1

10/18/2014
An unknown airborne environmental isolate on Bile Esculin Agar (BEA) exhibiting a single circular colony. White hyphal growth with no spore formation. Clear to brownish exudate can be seen throughout the colony. This sample grew at refrigerated temperatures for several months. The agar surrounding the colony did turn ...

Gelatin Hydrolysis

10/16/2014
Pseudomonas aeruginosa, Escherichia coli and Serratia marcescens were inoculated into gelatin agar using the stab method and incubated for 1 week at 37 degree’s C. If the organism has gelatinase then it can break down the gelatin (protein) into polypeptides and then amino acids which can then be ...

Temperature

09/28/2014
(A) Staphlococcus aureus, (B) Bacillus stearothermophilus, (C) Pseudomonas fluorescens and (D) Escherichia coli were grown on TSA for 24 hrs at varying incubator temperatures (4, 25, 40, and 60 degree’s C). Note: The 4 degree plate were incubated for a week to get good Pseudomonas fluorescens growth.

Osmotic pressure

09/28/2014
(A) Bacillus subtilis, (B) Staphlococcus aureus and (C) Escherichia coli were grown on BHI media with varying concentrations of NaCl (0.85%, 5%, 7.5%, 10%, and 20%) at 37 degrees C for 24 hrs.

Osmotic pressure

09/28/2014
A) Bacillus subtilis, (B) Staphlococcus aureus and (C) Escherichia coli were grown on BHI media with varying concentrations of NaCl (0.85%, 5%, 7.5%, 10%, and 20%) at 37 degrees C for 24 hrs.

Osmotic pressure

09/28/2014
A) Bacillus subtilis, (B) Staphlococcus aureus and (C) Escherichia coli were grown on BHI media with varying concentrations of NaCl (0.85%, 5%, 7.5%, 10%, and 20%) at 37 degrees C for 24 hrs.

6.5% NaCl Media

08/15/2014
Important ingredients: 6.5% salt, dextrose, bromcresol purple Differential/Selective: used to test for salt tolerance and can be used to identify enterococcal group D streptococci. NaCl is a selective agent and those organisms that can grow in higher salt concentrations also ferment the dextrose in the media. This causes acid ...

Methyl Red/Vogoes-Proskauer (MR-VP)

07/20/2014
Methyl Red/Vogoes-Proskauer (MR-VP) One medium used for two separate but related tests, identifying butanediol (VP) or acidic fermentation products (MR) as part of the IMViC test used in the identification of Enterobacteriaceae. •Methyl Red (MR): detects the ability of an organism to produce acidic fermentation products. Acidic procucts produce a red ...

SIM Media

07/13/2014
Sulfur-indole-motility test (SIM media) results for: (A) Escherichia coli: Motile***, hydrogen sulfide (-), indole (+)* (B) Staphylococcus aureus: Non-motile, hydrogen sulfide (-), indole (-) (C) Salmonella arizonae: Motile, hydrogen sulfide (+)**, indole (-) (D) Enterobacter aerogenes: Motile, hydrogen sulfide (-), indole (-) (E) Proteus vulgaris: Motile, hydrogen sulfide(+), indole (+) *After addition of 5-10 drops ...

Bacillus subtilis

07/04/2014
Gram stain of B. subtilis showing characteristic G +streptobacillus morphology.

Streptococcus pyogenes Gram Stain

06/27/2014
Gram stain done on S. pyogenes showing the characteristic long G+ streptococcus chains. Culture grown on blood agar for 48 hrs at 37 degree’s C.

Mycobacterium smegmatis Acid Fast Stain

06/27/2014
Acid Fast stain done on a mix of Staphylococcus aureus and Mycobacterium smegmatis. Carbol fuchsin was applied to the smear and set over a steaming water bath for 10 min to help penetrate the mycolic acid in the cell wall, then rinsed with acid alcohol which washes the ...

Endospore Stain Bacillus cereus

06/26/2014
B. cereus was grown on urea agar for 6 days at 37 degrees C to induce spore formation. The endospore stain was done using malachite green as the primary stain and safranin as counter the stain. Malachite green was applied to the smear and set over a steaming water bath ...

Starch Agar

06/18/2014
Starch agar contains high molecular weight starch molecules (soluable starch). When Iodine is added it reacts with starch to produces a dark brown/purple color. A zone of clearing forms around organisms that produce the exoenzyme amylase after addition of iodine. This plate was incubated for 24 hrs ...

Fluid Thioglycollate Media

06/03/2014
Organisms grown in Fluid Thioglycollate Media w/out resazurin dye is a reducing media that uses sodium thioglycollate to chemically combine with dissolved oxygen to deplete the oxygen in the media. All organism except for Neisseria sicca were grown for 48 hours at 37 degrees C. Neisseria sicca is a slow ...

Enterobacter aerogenes on MacConkey Agar (Mac)

05/24/2014
Streak plate isolation of Enterobacter aerogenes grown on MacConkey agar for 24 hours at 37 degree's. Colonies grow pink due to fermentation of lactose.

MaConkey Agar (Mac)

05/24/2014
Organisms grown on MacConkey Agar at 37 degrees for 24 hours. -Important Ingredients: crystal violet, bile salts, neutral red (color indicator), lactose -Differential: used to identify and isolate lactose fermenting G- enteric bacteria mainly Enterobacteriaceae. Strong lactose fermentation produces pink colonies with bile salt precipitation (hot pink). Weak fermentation produces ...

E.coli on MacConkey Agar (Mac)

05/23/2014
Streak plate isolation of E. coli on MacConkey Agar grown for 24 hrs at 37 degrees. E. coli demonstrates strong lactose fermentation indicated by the bright pink halo, bile precipitant around the colonies, and pink colony growth.

Unknown Agar Contaminate

05/18/2014
Unknown contaminant found on a TSA plate after being in the refrigerator for 1 month. Organism had grown throughout the media, was pale yellow and mucoid.

Unknown Contaminante

05/18/2014
Unknown contaminant found on a TSA plate after being in the refrigerator for 1 month. Organism had grow throughout the media.

Mannitol Salt Agar (MSA)

05/18/2014
Mannitol Salt Agar (MSA) •Important Ingredients: Phenol red, mannitol (sugar), 7.5% NaCl •Differential: between pathogenic S. aureus and S. epidermidis •Selective: NaCl inhibits non-halophilic organisms. Organism that can grow are halophilic. •Mannitol Fermentors: fermentation of acid causes a drop in pH of the media and the indicator (phenol red) turns yellow. A). ...

Mannitol Salt Agar (MSA)

05/18/2014
Mannitol Salt Agar (MSA) •Important Ingredients: Phenol red, mannitol (sugar), 7.5% NaCl •Differential: between pathogenic S. aureus and S. epidermidis •Selective: NaCl inhibits non-halophilic organisms. Organism that can grow are halophilic. •Mannitol Fermentors: fermentation of acid causes a drop in pH of the media and the indicator (phenol red) turns yellow. A). ...

Bacitracin test on Streptococcus pyogenes

05/15/2014
Bacitracin test done on a lawn of Streptococcus pyogenes grown on blood agar. The zone of inhibition around the bacitracin disc, approx 14mm measuring the entire length of the zone, indicates sensitivity. The zone of inhibition is red because the red blood cells did not lyse. ...

Streptococcus mitis on blood agar

05/15/2014
Growth of Streptococcus mitis on blood agar demonstrating alpha hemolysis seen as a greenish color around the growing colonies due to a reduction of the hemoglobin to methemoglobin in the surrounding agar. Image taken using transmitted light.

Mycobacterium smegmatis using transmitted light

05/07/2014
Isolated colony of Mycobacterium smegmatis grown on TSA for 96 hrs at 37 degrees C. Margin is lobate with an irregular-rhizoid form. Image taken using transmitted light.

Streptococcus pyogenes on blood agar

05/06/2014
Streak plate of Streptococcus pyogenes (incubated @ 37 degrees C for 48 hrs) showing beta hemolysis, complete lysis of red blood cells, around the isolated colonies. Image taken using transmitted light.

DNase Agar

04/29/2014
DNase agar can be used to differentiate between potentially pathogenic S. aureus and other Staphlococcus by looking for the presense of the exoenzyeme DNase. After growth for 24-48 hrs 1N HCL is added to the agar plate. Addition of HCL precipitates DNA in the media causing cloudiness ...

Kirby-Bauer disk diffusion

04/28/2014
Kirby-Bauer disk diffusion susceptibility test on coagulase-negative Staphylococcus aureus and E.coli grown on Mueller-Hinton agar with tetracycline (30 µg), cephalothin (30 µg), erythromycin (15 µg), chloramphenicol (30 µg), vancomycin (30 µg), penicillin (10 µg), streptomycin (10 µg), and novobiocin (30 µg). Staphylococcus aureus and E.coli were chosen to ...

Lipid Agar

04/28/2014
Lipid Plate/Tributyrin Agar used to test for an organisms ability to produce the exoenzyme lipase which breaks down the lipids in the agar creating a clear zone around the organism. (A) Serratia marcesens, lipid hydrolysis, indicated by a zone of clearing around the growing colony, as well as the red ...

Casein Media

04/24/2014
Casein Plates contain the major milk protein found in milk. Organisms were grown on Casien agar for 72 hours at 37o C to look for the presence of the exoenzyme proteases/caseases . (A): Pseudomonas aeruginosa, casein hydrolysis, as noted by a zone of clearing around the organism, as ...

Micrococcus luteus line inoculation

04/23/2014
Line inoculation of Micrococcus luteus on a TSA slant showing Filiform, uniform growth, along the margin. Culture was grown for 3 days at 37 degrees, M. luteus usually takes 3+ days for good growth.

Mycobacterium smegmatis line inoculation

04/19/2014
Line inoculation of Mycobacterium smegmatis on a TSA slant showing friable, dry crusty, growth. Culture was grown for 3 days at 37 degrees.

Yellowstone Caldera, Wyoming

04/15/2014
Bacterial mats, golden brown in color, seen at the outer edges of Chromatic Pools. The blue water in the center is the hottest water at ~199 F and cooling to ambient the farther you get from the center. Cyanobacteria grow around 163 F or lower.

Bacterial Mat

04/14/2014
Yellowstone caldera, Wyoming. Golden brown thermophilic cyano-bacteria seen in the waters surrounding one of the hydrothermal vents.

Alcaligenes faecalis

04/13/2014
Streak plate of Alcaligenes faecalis grown on TSA for 48 hr.

Physarum polycephalum Different view

04/09/2014
Physarum polycephalum, slime mold, grown in a large perti plate on moist paper towels using oatmeal as the food source. Culture was grown in the dark at room temperature. The paper towel was moistened every day with tap water. After 3 week’s the culture formed sporangia (fruiting bodies). Image was ...

Tube morphology

04/06/2014
Three organisms inoculated in TSB to look at tube morphology. From left to right: Staphylococcus aureus: flocculent growth/turbid, growth throughout the tube. Mycobacterium smegmatis: pellicle, growth at the top of the tube. M. smegmatis tends to stick to the tube and grow up the side. Bacillus subtilus: sediment, ...

Blood agar/Hemolysis

04/06/2014
Three organisms inoculated onto blood agar, straight line inoculation, to demonstrate hemolysis. From top to bottom: Streptococcus pyogenes: beta, complete lysis of red blood cells, clear area around colony growth. Streptococcus bovis: alpha, incomplete lysis of red blood cells, green area around colony growth. Enterococcus faecalis: gamma, growth ...

Mycobacterium smegmatis

04/05/2014
Streak plate of Mycobacterium smegmatis grown on TSA for 96 hrs.

M. luteus/E.coli

04/05/2014
Streak plate isolation of Micrococcus and E. coli mixed culture.

Bacillus subtilis

03/30/2014
Streak plate isolation showing colonial morphology of Bacillus subtilis on trypticase soy agar (TSA) incubated for 24 hours at 37oC. Note the rough and dry/matte texture characteristic of this organism’s growth.

Unknown Floor isolate #2

03/30/2014
Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and mounding in an irregular-rhizoid form with lobate margins. The colony exhibited an opaque yellow-orange pigment with a mucoid surface. This sample was obtained from a floor swab and incubated at 37°C.

Unknown floor isolate

03/30/2014
Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and an irregular-rhizoid form with lobate margins. The pigment is opaque white-beige with a mucoid surface over most of the colony. A dry, dull, matte surface appears toward the outer edges of the colony. The ...

Environmental Swab/Bottom of Shoe #2

03/26/2014
Close up view of the edges of an unknown bacteria swabbed form the bottom of a shoe, possible Bacillus, on TSA exhibiting spreading and rhizoid growth. The pigment is opaque white-beige with a mucoid surface towards the center of the colony and a matte surface towards the edges. ...

Environmental Swab/Bottom of Shoe #1

03/26/2014
On the first day of Micro class instructors have students do an environmental swab. This sample was subcultured from a student who did the bottom of their shoe. The organism, possible Bacillus sp., was tough to scrap off the agar so subculture was done by taking a ...

Airborne Environmental Isolate #3

03/26/2014
An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the red/pink grainy spore formation in the center of the colony. This sample grew at refrigerated temperatures for ...

Airborne Environmental Isolate #2

03/26/2014
An unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth on the outer edges of the mold colony, where spores have not yet developed, surrounding the green and yellow grainy spore formation in the center of the colony with exudate in the center. ...

Airborne Environmental Isolate

03/26/2014
Media is poured in bulk in our lab for classroom use. Although contamination is low we do see airborne contamination especially durning the rainy season. This is an unknown airborne environmental isolate on Mueller-Hinton agar exhibiting a single circular colony. White hyphal growth can be seen throughout the ...

Mycobacterium smegmatis

03/24/2014
Mycobacterium smegmatis grown on TSA for 96 hrs.

Mycobacterium smegmatis

03/24/2014
Mycobacterium smegmatis grown on TSA for 96 hrs.

Serratia marcescens/Escherichia coli mix

03/24/2014
Serratia marcescens/Escherichia coli mix grown on TSA for 48 hrs. Serratia marcescens grows red due to prodigiosin and Escherichia coli grows opaque white.

Micrococcus luteus

03/24/2014
Micrococcus luteus grown on TSA for 96 hrs.

Physarum polycephalum

03/21/2014
Physarum polycephalum, slime mold, grown in a large perti plate on moist paper towels using oatmeal as the food source. Culture was grown in the dark at room temperature. The paper towel was moistened every day with tap water. After 3 week’s the culture formed sporangia (fruiting ...
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