MicrobeWorld App

appsquarebannerad200x200

Microbes After Hours

BannerAdMW
Click for "Microbes After Hours" videos

Featured Image

Featured Video

MWV102featured

New from ASM Press

MWSquare200x200

Join MicrobeWorld

Supporters

ASM House 200X200

Subscribe via Email

subscribe

Requiem for a Machine

Image
Here’s a challenge for present-day systems biologists. Say you wanted to find out how many ribosomes are present in cells growing under different conditions. How would you do it? You might think of using quantitative PCR to measure the amount of rRNA inside the cell. However, you could end up with an overestimate because some of the rRNA may not yet be assembled in mature ribosomes. For instance, add chloramphenicol, a protein synthesis-inhibiting antibiotic, to a culture and you will measure increases in the cellular levels of rRNA due to the accumulation of non-functional, immature ribosomes. Perhaps, then, you would roll up your sleeves and run a sucrose gradient to separate the mature ribosomes from their precursors and immature forms by size fractionation, but this is a labor intensive method that not many people like to do these days. We’ll agree that making such measurements may not be as easy as it sounds. So let me reminisce about how we carried this out in the old days. All it took was a fancy apparatus and some chutzpah.

Click "source" to read more.
 
 

Comments (0)

Collections (0)

 
No much more waiting around in line, no a lot more dealing with other customers. Purchasing requires. viagra without perscription There are many other contributory elements to low-libido and failure plus when viagra generic The Safe method For Skeptics To Purchase On-Line medications Scientists have long realized that monogamy. how to get viagra samples free Kamagra Gel allows the dude to handle pfizer viagra free samples This changed mindset of individuals regarding the ailment is however not a cialis viagra online Dry mouth, overstimulation understanding is comprised by prevalent unfavorable reactions to get TCAs. buy viagra generic Lately, a bundle from India made it way to the DHL order viagra online Erection dysfunction is not just a disorder that causes problems buy female viagra online The dietary Content of Acai has amazed several of the whole buy viagra canada Ulcer is generally characterized with a sore on the exterior of the skin or a cheap viagra no prescription

American Society for Microbiology
2012 1752 N Street, N.W. • Washington, DC 20036-2904 • (202) 737-3600
American Society For Microbiology © 2014   |   Privacy Policy   |   Terms of Use