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Microscope technology sees sub-cellular in 3D

A new advance in microscopy offers fast, detailed, 3D views of cells’ internal structures without the use of fluorescence or contrast agents.

In a paper published in the journal PLoS ONE, researchers at the University of Illinois who developed the technique report they were able to use it to visualize the E. coli bacteria with a combination of speed, scale, and resolution unparalleled for a label-free method.

The method is based on a broadband interferometric technique known as Spatial Light Interference Microscopy (SLIM) that was designed by Gabriel Popescu, a research at the University of Illinois’ Beckman Institute, as an add-on module to a commercial phase contrast microscope.

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