MicrobeWorld App

appsquarebannerad200x200

Join MicrobeWorld

Subscribe via Email

subscribe

Microbes After Hours

cheese-thumb-small

Click for more "Microbes After Hours" videos

Featured Image

Featured Video

Crowdsourced Microbes Heading to Station

Supporters

ASM House 200X200

Microbiology Dilution Series Demonstration














Here's a nice demonstration video by Dr. Conor O'Bryne from the Dept. of Microbiology at National University of Ireland, Galway, showing how to perform a serial dilution on a liquid food sample (in this case raw unpasteurised milk). The raw milk sample is diluted down to 1/1000 in a sterile diluent (Ringers solution). 1ml samples of each dilution are then used to prepare 2 sets of pour plates. A 0.1ml sample from the 1/10 and the 1/100 dilutions is used to generate spread plates.

For this method you require the following:

Sterile 1ml pipettes
A pipette pump
Sterile agar plates (for pour plates)
Poured agar plates (for spread plates)
A spreader (glass or metal)
A dish of lab ethanol
Bunsen burner
Glass Universal tubes with 9 ml of sterile diluent
Molten agar medium (20ml plate count agar per Universal tube - kept at 48C until ready to pour)
 
 

Comments (0)

Collections (0)

 

American Society for Microbiology
2012 1752 N Street, N.W. • Washington, DC 20036-2904 • (202) 737-3600
American Society For Microbiology © 2014   |   Privacy Policy   |   Terms of Use