Microbial communities in the Gulf of Mexico have surely been impacted by the oil disaster over the last couple of weeks. Labs are now beginning to assess the damage done by collecting water onto filter membranes and shipping the filters back to their labs for DNA analysis.
A frequent question we've heard in our lab is how to store water filters collected in the field for later extraction of DNA in the lab. What is the best practice? Today’s article will share our advice based on the research we have performed in this area. Here is an email from a researcher at the University of Georgia sent to us this week and our answer.
I am looking for suggestions for storing filters for subsequent DNA extraction using your PowerWater kit. We are heading out this weekend to the Gulf of Mexico and Florida Keys and will be collecting water samples for microbial analysis. We will be able to filter in the field but not complete the extraction process. Additionally our freezer space is limited. Do you have suggestions for storage of the filters that is not dependent on freezing. Is EtOH a possibility (will it work with the extraction process downstream?) This is a last minute trip and we are trying to work with materials at hand as best we can.
Thanks for your insight.
To see the answer and learn more about what works and what doesn't, click the Source link.