Ideally, the efficiency of the assay should be 100%. This means that during the logarithmic phase of the reaction, the PCR product of interest is doubling with each cycle. Perfect PCR efficiency will demonstrate a change of 3.3 cycles between 10 fold dilutions of template.
Sometimes the efficiency is below 100% and sometimes you can get readings of PCR efficiency above 100%. In a future article, we’ll discuss what causes high and low PCR efficiencies and how to fix it.
Today’s article is about the important considerations when setting up a PCR efficiency test for your new assay. The three key ingredients to focus on when getting started are the type of template, the primers, and the chemistry.
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