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Journal Club: Get Rid of False-Positives in 16s rDNA PCR

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How many of you run into problems of false- positive PCR or lack of sensitivity in qPCR when trying to use 16S primers because of the background genomic DNA in your PCR enzyme mixes? At MO BIO Labs, we do.
This article is a review of a new paper in the Journal of Microbiological Methods (November 2009) on a method for pre-treating your enzyme mix to remove bacterial genomic DNA.

Leave a comment if you want to discuss the pros and cons of this method or let us know how you overcome this problem.
 
 

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