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A Plethora of Phenotypes

My narration in this video might have been a little more accurate, so I'll clarify here:

Essentially we are trying to make a protein in B. megaterium fluoresce with GFP, green fluorescent protein. We are doing so by transforming it with a plasmid that gives resistance to Tetracycline, an antibiotic, as well as the desired characteristic. I've noticed that every time I streak out a new plate of this stuff (including the example above) the morphology changes noticably. The bacterial strain of B. megaterium that we are using is very mucoid in a liquid broth, so it seems likely that the non-uniform colonies are due to the bacterium's ability to change it's mucus production in relation to its environment.

If you'd like to contact me, I'm @loyaleagle on Twitter :)

Coming to you from the University of Maryland, College Park.

Comments (2)

  1. Hey Jimmy this is a very cool, do-it-yourself, behind the scenes video! I look forward to seeing more of this type of content. It's cool to see into your lab and get a personal perspective of your work.
  2. Thanks Ray...I'm glad that ASM has really gone out there and done some neat stuff with new media :)

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