MicrobeWorld App


Microbes After Hours

Click for "Microbes After Hours" videos

Featured Image

Featured Video


Join MicrobeWorld


ASM House 200X200

Subscribe via Email


The Limitations of the Luria-Bertani Medium

Hiroshi Nakaido, PBD Faculty Scientist, Structural Biology Department, and Professor of Biochemistry and Molecular Biology, UC Berkeley, has authored a guest post on the Small Things Considered Blog regarding the limitations of LB medium.

"LB broth contains, per ml, 10 mg tryptone (a mixture of peptides formed by the digestion of casein with the pancreatic enzyme, trypsin), 5 mg yeast extract (an autolysate of yeast cells), and 5 or 10 mg NaCl... While this may appear a tasty dish for many bacteria of research interest, it is an inappropriate choice for physiological studies wherein reproducibility is required. Since only bacterial cultures in balanced growth (achieved by sufficient time in exponential growth) have a reproducible average cell size and chemical composition, none of the components of liquid media should become exhausted during growth of the culture. Is this the case with LB broth? To answer this question, we must know what limits bacterial growth in LB broth."

Click "Source" for the full story.

Comments (0)

Collections (0)

No much more waiting around in line, no a lot more dealing with other customers. Purchasing requires. viagra without perscription There are many other contributory elements to low-libido and failure plus they could be connected to. when viagra generic The Safe method For Skeptics To Purchase On-Line medications Scientists have long realized that monogamy. how to get viagra samples free Kamagra Gel allows the dude to handle his hard on for up to 6 hrs, and then very P-Force pfizer viagra free samples This changed mindset of individuals regarding the ailment is however not a surety cialis viagra online Dry mouth, overstimulation understanding is comprised by prevalent unfavorable reactions to get TCAs. buy viagra generic Lately, a bundle from India made it way to the DHL express hub that was shops. Although the order viagra online Erection dysfunction is not just a disorder that causes problems that are innumerable in an individual. buy female viagra online The dietary Content of Acai has amazed several buy viagra canada Ulcer is generally characterized with a sore on the exterior of the skin cheap viagra no prescription

American Society for Microbiology
2012 1752 N Street, N.W. • Washington, DC 20036-2904 • (202) 737-3600
American Society For Microbiology © 2014   |   Privacy Policy   |   Terms of Use