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Which is the morphology of this micro-organism?

IMG_7137.jpg
Please help me! I am planning to identify this microorganism by API but I can not identify the shape (rod or cocci). This microorganism has positive oxidase. Its colony is red, irregular, flat.
Please teach me. Thanks so much! Nhu Thuy from Vietnam
 
 

Comments (3)

  1. Hi, it looks to me either not enough bacteria on the slide or maybe the slide was flamed too much. I would recommend if you're performing a gram stain to place the smallest amount of water as a tiny drop on the slide. Then grab a colony and smear it on there and let it dry for a bit before flaming it. Also have positive controls on the same slide as in E. Coli and Staphylococcus to compare the morphology. In the end the slide would have staph, unknown in the middle and E. coli at the end of the slide. Also see if you microscope can do a oil immersion too.
  2. Yes, please redo the gram stain. If you'd like do a wet mount with a cover slip of the microorganism and watch it and determine if it is a rod or cocci. Continue with the gram stain to see if it is gram positive or gram negative. You can continue the identification from then on. Good luck!
  3. Thank you so much for your advice, Jaimes and Kurbessoian. I will do gram stain with your advice. ^.^

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