MicrobeWorld App


Microbes After Hours

Click for "Microbes After Hours" videos

Featured Image

Featured Video


New from ASM Press


Join MicrobeWorld


ASM House 200X200

Subscribe via Email


D- Zone Test for phenotypic detection of Inducible clindamycin Resistance

A positive D test indicates that the presence of macrolide-inducible resistance to clindamycin produced by an inducible methylase enzyme that alters the common ribosomal binding site for macrolides, clindamycin and the group B streptogrammins (quinupristin). This cross-resistance, called the MLS-B phenotype, has been associated with clinical reports of clindamycin failure.

The media used for the detection of phenotypic inhibition test is cation adjusted Mueller Hinton Agar (MHA). The inoculum density of bacteria (Staphylococcus aureus) was first adjusted to 0.5 Mc Farland Standard and then plated into the MHA plate. Clindamycin(2µg) and Erythromycin(15µg) disks were placed at 25 mm distance from each other and the plate was incubated at 370C in aerobic condition for 24 hours. The result shows complete Erythromycin resistance and obliteration of Clindamycin zone of inhibition on the side of Erythromycin with the formation of semilunar(D) structure or D shape known as D Zone Test.

By :
Narayan Parajuli
M Sc Clinical Microbiology
Institute of Medicine
Kathmandu, Nepal

Comments (0)

Collections (0)

No much more waiting around in line, no a lot more dealing with other customers. Purchasing requires. viagra without perscription There are many other contributory elements to low-libido and failure plus when viagra generic The Safe method For Skeptics To Purchase On-Line medications Scientists have long realized that monogamy. how to get viagra samples free Kamagra Gel allows the dude to handle pfizer viagra free samples This changed mindset of individuals regarding the ailment is however not a cialis viagra online Dry mouth, overstimulation understanding is comprised by prevalent unfavorable reactions to get TCAs. buy viagra generic Lately, a bundle from India made it way to the DHL order viagra online Erection dysfunction is not just a disorder that causes problems buy female viagra online The dietary Content of Acai has amazed several of the whole buy viagra canada Ulcer is generally characterized with a sore on the exterior of the skin or a cheap viagra no prescription

American Society for Microbiology
2012 1752 N Street, N.W. • Washington, DC 20036-2904 • (202) 737-3600
American Society For Microbiology © 2014   |   Privacy Policy   |   Terms of Use