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Vibrio cholerae serotype O1 biotype ogawa isolated on TCBS agar in Sukraraj Tropical and Infectious Hospital , Kathmandu , Nepal .

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Vibrio cholerae was isolated in the capital city Kathmandu , where as it causes cholerae endemic in certain remote parts in the country every year . Vibrio cholerae O1 ogawa serotype was isolated and identified after growth in TCBS agar and subcultured on HIA plate and performed oxidase test (+) and TSI (a/a) . Then it was performed slide agglutination test with polyvalent antisera which showed positive test and further tested with ogawa antisera which also showed positive test .
 
 

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