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colony pic. of V.cholerae in the form of ASM(American society for microbiology).

this isolate is v. cholerae o1,so colony pic of v. cholerae written as ASM(american society for microbiology) in TCBS(thiosulphate citrate bile salt sucrose) media as sucrose fermenting yellow colonies.
organisms: V. cholerae o1
media:TCBS(thiosulphate citrate bile salt sucrose) media
incubation condition:at 37'c for 24hrs.
Gram stain -Gram negative, curved rods
colony morphology: sucrose fermenting large yellow colonies.
Vibrio cholerae is a Gram-negative, comma-shaped bacterium.

Biochemical Reactions:
Catalase/Oxidase- both positive
Citrate- positive
Indole- Positive(late)
Urea hydrolysis-Negative
Motility- Motile(Darting motility)
Methyl Red-negative
Vogues Proskaeur- Negative
TSI- Acid /Acid (Y/Y) without gas and H2S.
LAO decarboxylase- (+ - +)

cls sundar khadka,
PG in clinical microbiology,8th batch
institute of medicine(IOM), TU Teaching hospital, Nepal.

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