this isolate is v. cholerae o1,so colony pic of v. cholerae written as ASM(american society for microbiology) in TCBS(thiosulphate citrate bile salt sucrose) media as sucrose fermenting yellow colonies.
organisms: V. cholerae o1
media:TCBS(thiosulphate citrate bile salt sucrose) media
incubation condition:at 37'c for 24hrs.
Gram stain -Gram negative, curved rods
colony morphology: sucrose fermenting large yellow colonies.
Vibrio cholerae is a Gram-negative, comma-shaped bacterium.
Catalase/Oxidase- both positive
Motility- Motile(Darting motility)
Vogues Proskaeur- Negative
TSI- Acid /Acid (Y/Y) without gas and H2S.
LAO decarboxylase- (+ - +)
cls sundar khadka,
PG in clinical microbiology,8th batch
institute of medicine(IOM), TU Teaching hospital, Nepal.