india ink preparation for cryptococcus neoformans.
this organisism is isolated from suspected of cryptococcosis from HIV infected patient, 45yrs/M.this work is performed at , deparment of Mycology at TU, Teaching Hospital, Ktm, nepal.
Phenotypic test done for conformation of this organisms.
1. india ink prep: yeast cell and budding yeast cell seen.
2. physiological n biochemical test: Urea hydrolysis test: positive after 48hrs of incubation at 37'c.
3. blood agar: grown of yeast cell after 48hrs of incubation at 37'c.
so, this organisms ia reported as cryptococcous neoformans.
Molecular techinque: remaining.
cls. sundar khadka,
department of autonomous clinical mycology ,
TU Teaching hospital,Nepal.