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INDIA INK Preparation for Cryptococcus .

india ink preparation for cryptococcus neoformans.

this organisism is isolated from suspected of cryptococcosis from HIV infected patient, 45yrs/M.this work is performed at , deparment of Mycology at TU, Teaching Hospital, Ktm, nepal.
Phenotypic test done for conformation of this organisms.
specimens: CSF

1. india ink prep: yeast cell and budding yeast cell seen.
2. physiological n biochemical test: Urea hydrolysis test: positive after 48hrs of incubation at 37'c.
3. blood agar: grown of yeast cell after 48hrs of incubation at 37'c.

so, this organisms ia reported as cryptococcous neoformans.

Molecular techinque: remaining.


cls. sundar khadka,
department of autonomous clinical mycology ,
TU Teaching hospital,Nepal.

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