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Mycobacterium smegmatis Acid Fast Stain

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Acid Fast stain done on a mix of Staphylococcus aureus and Mycobacterium smegmatis. Carbol fuchsin was applied to the smear and set over a steaming water bath for 10 min to help penetrate the mycolic acid in the cell wall, then rinsed with acid alcohol which washes the carbol fuchsin out of all but the acid fast cells. Methylene blue was then used as the counter stain. M. smegmatis (acid fast) stained bright pink and S. aureus blue.
 
 

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